2018
Quantitative susceptibility mapping identifies inflammation in a subset of chronic multiple sclerosis lesions
Kaunzner UW, Kang Y, Zhang S, Morris E, Yao Y, Pandya S, Rua S, Park C, Gillen KM, Nguyen TD, Wang Y, Pitt D, Gauthier SA. Quantitative susceptibility mapping identifies inflammation in a subset of chronic multiple sclerosis lesions. Brain 2018, 142: 133-145. PMID: 30561514, PMCID: PMC6308309, DOI: 10.1093/brain/awy296.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedAntigens, CDAntigens, Differentiation, MyelomonocyticBrainCarbon RadioisotopesChronic DiseaseCross-Sectional StudiesFemaleHumansInflammationIronIsoquinolinesMacrophagesMagnetic Resonance ImagingMaleMicrogliaMiddle AgedMultiple SclerosisPositron-Emission TomographyRetrospective StudiesYoung AdultConceptsChronic active lesionsMultiple sclerosisChronic lesionsActive lesionsMultiple sclerosis lesionsHyperintense rimQuantitative susceptibility mappingChronic active multiple sclerosis lesionsSclerosis lesionsChronic multiple sclerosis lesionsActive multiple sclerosis lesionsPersistent inflammatory activityProgressive multiple sclerosisMicroglia/macrophagesInnate immune activationEarly disease stagesTranslocator proteinGreater tissue damagePost-mortem studiesProgressive patientsActivated microgliaInflammatory activityPersistent inflammationImmune activationDisease stage
2016
Iron in Multiple Sclerosis and Its Noninvasive Imaging with Quantitative Susceptibility Mapping
Stüber C, Pitt D, Wang Y. Iron in Multiple Sclerosis and Its Noninvasive Imaging with Quantitative Susceptibility Mapping. International Journal Of Molecular Sciences 2016, 17: 100. PMID: 26784172, PMCID: PMC4730342, DOI: 10.3390/ijms17010100.Peer-Reviewed Original ResearchConceptsMultiple sclerosisMagnetic resonance imagingBrain tissueQuantitative susceptibility mappingMS brain tissueAdvanced MRI methodsMS patientsChronic inflammationImmunohistochemical investigationBrain ironMyeloid cellsResonance imagingNon-invasive studyHistological studyRole of ironOxidative stressNoninvasive imagingSclerosisInflammationCellular distributionMRI methodsNeurodegenerationTissueImagingPatients
2015
Biomarkers in multiple sclerosis
Housley WJ, Pitt D, Hafler DA. Biomarkers in multiple sclerosis. Clinical Immunology 2015, 161: 51-58. PMID: 26143623, DOI: 10.1016/j.clim.2015.06.015.Peer-Reviewed Original ResearchConceptsMultiple sclerosisB cell chemoattractant CXCL13Myelin-reactive T cellsMacrophage marker CD163Reactive T cellsMarkers of neurodegenerationKIR4.1 antibodiesMS seraClinical outcomesOligoclonal bandsYKL-40Disease progressionT cellsMS susceptibilityCerebrospinal fluidPotential biomarkersViral titersClinical useBiomarkersBiomarker researchSclerosisProgressionDisease diagnosisCD163CXCL13
2014
B cells populating the multiple sclerosis brain mature in the draining cervical lymph nodes
Stern JN, Yaari G, Vander Heiden JA, Church G, Donahue WF, Hintzen RQ, Huttner AJ, Laman JD, Nagra RM, Nylander A, Pitt D, Ramanan S, Siddiqui BA, Vigneault F, Kleinstein SH, Hafler DA, O'Connor KC. B cells populating the multiple sclerosis brain mature in the draining cervical lymph nodes. Science Translational Medicine 2014, 6: 248ra107. PMID: 25100741, PMCID: PMC4388137, DOI: 10.1126/scitranslmed.3008879.Peer-Reviewed Original ResearchConceptsCervical lymph nodesCentral nervous systemB cellsCerebrospinal fluidLymph nodesMultiple sclerosisLymphoid tissueCNS of patientsCNS B cellsAntigen-experienced B cellsMultiple sclerosis brainSecondary lymphoid tissuesB cell compartmentB cell trafficB cell maturationImmunomodulatory therapyImmune infiltratesPeripheral bloodInflammatory diseasesLymphocyte transmigrationPeripheral tissuesNervous systemMembers of clonesCell maturationCell traffic
2013
Iron Is a Sensitive Biomarker for Inflammation in Multiple Sclerosis Lesions
Mehta V, Pei W, Yang G, Li S, Swamy E, Boster A, Schmalbrock P, Pitt D. Iron Is a Sensitive Biomarker for Inflammation in Multiple Sclerosis Lesions. PLOS ONE 2013, 8: e57573. PMID: 23516409, PMCID: PMC3597727, DOI: 10.1371/journal.pone.0057573.Peer-Reviewed Original ResearchConceptsMyelin-laden macrophagesMS patientsSecondary progressive MS patientsProgressive MS patientsSecondary progressive MSMultiple sclerosis patientsMacrophages/microgliaWhite matter lesionsHuman macrophage culturesIron-containing macrophagesMultiple sclerosis lesionsImportant clinical informationHuman cultured macrophagesActive relapsingDemyelinating lesionsDisease-relevant processesProgressive MSDemyelinated lesionsSclerosis patientsMultiple sclerosisMatter lesionsM1 polarizationImmunohistochemical examinationMyelin phagocytosisProinflammatory polarization
2011
T1 and proton density at 7 T in patients with multiple sclerosis: an initial study
Bluestein KT, Pitt D, Knopp MV, Schmalbrock P. T1 and proton density at 7 T in patients with multiple sclerosis: an initial study. Magnetic Resonance Imaging 2011, 30: 19-25. PMID: 21937183, PMCID: PMC3375320, DOI: 10.1016/j.mri.2011.07.018.Peer-Reviewed Original ResearchConceptsCortical lesionsMultiple sclerosisWhite matterGray matterHealthy control subjectsWhite matter lesionsNormal-appearing tissueMS patientsMagnetic resonance sequencesMatter lesionsControl subjectsCerebrospinal fluidMS researchLesionsPatientsMR imagingUltrahigh-field MR imagingField MR imagingSclerosisProton densityResonance sequencesTissue responseTurbo fieldMagnetic resonanceInitial study
2009
Dysmyelinated axons in shiverer mice are highly vulnerable to α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor-mediated toxicity
Pitt D, Gonzales E, Cross AH, Goldberg MP. Dysmyelinated axons in shiverer mice are highly vulnerable to α-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptor-mediated toxicity. Brain Research 2009, 1309: 146-154. PMID: 19896473, PMCID: PMC7343376, DOI: 10.1016/j.brainres.2009.10.066.Peer-Reviewed Original ResearchMeSH KeywordsAlpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic AcidAnimalsBiomarkersBrainDisease Models, AnimalExcitatory Amino Acid AgonistsFemaleHereditary Central Nervous System Demyelinating DiseasesLuminescent ProteinsMiceMice, Inbred C57BLMice, Neurologic MutantsMovement DisordersMyelin Basic ProteinNerve DegenerationNerve Fibers, MyelinatedNeurotoxinsN-MethylaspartateReceptors, AMPAConceptsNMDA receptorsShiverer miceAMPA/kainate receptorsLumbar dorsal columnWhite matter injuryWidespread axonal degenerationSpinal cord axonsActivation of receptorsReceptor-mediated toxicitySubset of axonsMyelin basic proteinAxonal vulnerabilityNeuroprotective therapiesGlutamate excitotoxicityNMDA injectionAxonal degenerationAxonal injuryDorsal columnsRotarod performanceAxon damageGlutamate toxicityCentral axonsGlial cellsS-AMPAAxonal toxicity
2001
Multiple sclerosis: Altered glutamate homeostasis in lesions correlates with oligodendrocyte and axonal damage
Werner P, Pitt D, Raine C. Multiple sclerosis: Altered glutamate homeostasis in lesions correlates with oligodendrocyte and axonal damage. Annals Of Neurology 2001, 50: 169-180. PMID: 11506399, DOI: 10.1002/ana.1077.Peer-Reviewed Original ResearchConceptsMultiple sclerosisAxonal damageWhite matterGlutamate excitotoxicityGlutamate homeostasisMS lesionsGlutaminase expressionMS white matterInflammatory neurologic diseasesActive MS lesionsCNS cell typesOligodendroglial pathologyNoninflammatory conditionsDystrophic axonsNeurologic diseaseGLT-1Low-level expressionAnimal modelsGlutamate transportersHomeostasis contributesLesion correlatesGlutamate transportLesionsOligodendrocytesTherapeutic import
1999
Neuregulin and erbB receptor expression in normal and diseased human white matter
Cannella B, Pitt D, Marchionni M, Raine C. Neuregulin and erbB receptor expression in normal and diseased human white matter. Journal Of Neuroimmunology 1999, 100: 233-242. PMID: 10695733, DOI: 10.1016/s0165-5728(99)00201-5.Peer-Reviewed Original ResearchConceptsGlial growth factor 2Multiple sclerosisErbB receptor expressionWhite matterHuman white matterReceptor expressionCases of MSHuman lymph node tissueAreas of remyelinationLymph node tissueGrowth factor 2Autoimmune demyelinationMicroglial cellsNeurologic diseaseMS lesionsNode tissueMitogenic effectWestern blotWestern blottingOligodendrocytesReceptorsRemyelinationWidespread expressionFactor 2Lymphocytes