2014
NAP Reduces Murine Microvascular Endothelial Cells Proliferation Induced by Hyperglycemia
D’Amico A, Scuderi S, Maugeri G, Cavallaro S, Drago F, D’Agata V. NAP Reduces Murine Microvascular Endothelial Cells Proliferation Induced by Hyperglycemia. Journal Of Molecular Neuroscience 2014, 54: 405-413. PMID: 24874579, DOI: 10.1007/s12031-014-0335-2.Peer-Reviewed Original ResearchConceptsProtein kinase/extracellular signal-regulated protein kinaseExtracellular signal-regulated protein kinaseMitogen-activated protein kinase/extracellular signal-regulated protein kinaseSignal-regulated protein kinaseKinase/Akt pathwayEndothelial cell proliferationPhosphatidylinositol-3-kinase/Akt pathwayCell proliferationProtein expression analysisProtein kinaseExpression analysisBiological roleAkt pathwayPhosphorylation levelsTime-dependent mannerHigh glucoseMurine microvascular endothelial cellsMicrovascular endothelial cell proliferationMicrovascular endothelial cellsEndothelial cellsProliferationMTT assayCells
2012
Ameliorative effect of PACAP and VIP against increased permeability in a model of outer blood retinal barrier dysfunction
Scuderi S, D’Amico A, Castorina A, Imbesi R, Carnazza M, D’Agata V. Ameliorative effect of PACAP and VIP against increased permeability in a model of outer blood retinal barrier dysfunction. Peptides 2012, 39: 119-124. PMID: 23220033, DOI: 10.1016/j.peptides.2012.11.015.Peer-Reviewed Original ResearchMeSH KeywordsBlood-Retinal BarrierCapillary PermeabilityCell LineClaudin-1DextransDiabetes ComplicationsElectric ImpedanceFluorescein-5-isothiocyanateGene ExpressionGlucoseHumansInterleukin-1betaMacular EdemaOccludinPituitary Adenylate Cyclase-Activating PolypeptideRetinal Pigment EpitheliumTight JunctionsVasoactive Intestinal PeptideZonula Occludens-1 ProteinConceptsDiabetic macular edemaBlood-retinal barrierTransepithelial electrical resistanceBRB functionBlood-retinal barrier dysfunctionOuter blood-retinal barrierEffects of PACAPHuman retinal pigment epithelial cellsFITC-dextran diffusionRetinal pigment epithelial cellsZO-1 expressionTight junctionsPigment epithelial cellsMacular edemaMajor complicationsInflammatory aspectsRetinal barrierRetinal injuryBarrier dysfunctionIL-1βDiabetic retinopathyDiabetic milieuTJ-related proteinsOccludin expressionNormal glucose