2013
PACAP and VIP increase the expression of myelin-related proteins in rat schwannoma cells: Involvement of PAC1/VPAC2 receptor-mediated activation of PI3K/Akt signaling pathways
Castorina A, Scuderi S, D’Amico A, Drago F, D’Agata V. PACAP and VIP increase the expression of myelin-related proteins in rat schwannoma cells: Involvement of PAC1/VPAC2 receptor-mediated activation of PI3K/Akt signaling pathways. Experimental Cell Research 2013, 322: 108-121. PMID: 24246222, DOI: 10.1016/j.yexcr.2013.11.003.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCell Line, TumorEnzyme ActivationGene Expression Regulation, NeoplasticMyelin Basic ProteinMyelin P0 ProteinMyelin ProteinsMyelin-Associated GlycoproteinOncogene Protein v-aktPhosphatidylinositol 3-KinasesPituitary Adenylate Cyclase-Activating PolypeptideRatsReceptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type IReceptors, Vasoactive Intestinal Peptide, Type IISchwann CellsSignal TransductionVasoactive Intestinal PeptideConceptsPI3K/AktMyelin proteinsMyelin markersVIP receptor antagonistMyelin-related proteinsStarvation-induced expressionCanonical PI3K/AktSpecific pathway inhibitorsRat Schwannoma cell lineReceptor-mediated activationPI3K inhibitorsPhosphorylation of AktNM PACAPPACAP6-38Underlying molecular mechanismsEndogenous PACAPPhosphorylated ERK 1/2Schwannoma cell lineReceptor antagonistPeripheral expressionMyelin gene expressionRat schwannoma cellsPharmacological approachesSer473 residueNormal serumAntiproliferative Effects of PACAP and VIP in Serum-Starved Glioma Cells
D’Amico A, Scuderi S, Saccone S, Castorina A, Drago F, D’Agata V. Antiproliferative Effects of PACAP and VIP in Serum-Starved Glioma Cells. Journal Of Molecular Neuroscience 2013, 51: 503-513. PMID: 23900722, DOI: 10.1007/s12031-013-0076-7.Peer-Reviewed Original ResearchConceptsPituitary adenylate cyclase-activating polypeptideVasoactive intestinal peptideCalorie restrictionGlioma cellsCell proliferationEffects of PACAPAdenylate cyclase-activating polypeptideCyclase-activating polypeptideExpression of p53Expression of nestinFormation of metastasesC6 glioma cellsIntestinal peptideAcute conditionsVIP treatmentPrognostic markerExpression of proteinsCell malignanciesGFAP decreasesTumor microenvironmentAntiproliferative effectsCyclin D1Cancer developmentCancer treatmentCancer cells
2012
Ameliorative effect of PACAP and VIP against increased permeability in a model of outer blood retinal barrier dysfunction
Scuderi S, D’Amico A, Castorina A, Imbesi R, Carnazza M, D’Agata V. Ameliorative effect of PACAP and VIP against increased permeability in a model of outer blood retinal barrier dysfunction. Peptides 2012, 39: 119-124. PMID: 23220033, DOI: 10.1016/j.peptides.2012.11.015.Peer-Reviewed Original ResearchMeSH KeywordsBlood-Retinal BarrierCapillary PermeabilityCell LineClaudin-1DextransDiabetes ComplicationsElectric ImpedanceFluorescein-5-isothiocyanateGene ExpressionGlucoseHumansInterleukin-1betaMacular EdemaOccludinPituitary Adenylate Cyclase-Activating PolypeptideRetinal Pigment EpitheliumTight JunctionsVasoactive Intestinal PeptideZonula Occludens-1 ProteinConceptsDiabetic macular edemaBlood-retinal barrierTransepithelial electrical resistanceBRB functionBlood-retinal barrier dysfunctionOuter blood-retinal barrierEffects of PACAPHuman retinal pigment epithelial cellsFITC-dextran diffusionRetinal pigment epithelial cellsZO-1 expressionTight junctionsPigment epithelial cellsMacular edemaMajor complicationsInflammatory aspectsRetinal barrierRetinal injuryBarrier dysfunctionIL-1βDiabetic retinopathyDiabetic milieuTJ-related proteinsOccludin expressionNormal glucose