2014
NMDA and AMPA receptors contribute similarly to temporal processing in mammalian retinal ganglion cells
Stafford BK, Manookin MB, Singer JH, Demb JB. NMDA and AMPA receptors contribute similarly to temporal processing in mammalian retinal ganglion cells. The Journal Of Physiology 2014, 592: 4877-4889. PMID: 25217374, PMCID: PMC4259533, DOI: 10.1113/jphysiol.2014.276543.Peer-Reviewed Original ResearchConceptsGanglion cellsNMDAR kineticsOFF alphaMammalian retinal ganglion cellsNMDA-type glutamate receptorsAMPAR-mediated responsesOFF alpha cellsGanglion cell typesRetinal ganglion cellsGuinea pig retinaNMDAR-mediated responsesBipolar cell pathwaysCell typesFast excitatory neurotransmissionGlutamatergic transmissionPresynaptic inhibitionGlutamate releaseSame synapsesSynaptic inhibitionGlutamate receptorsHz stimulationPostsynaptic AMPAExcitatory neurotransmissionSensory neuronsAMPA receptorsKainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina
Borghuis BG, Looger LL, Tomita S, Demb JB. Kainate Receptors Mediate Signaling in Both Transient and Sustained OFF Bipolar Cell Pathways in Mouse Retina. Journal Of Neuroscience 2014, 34: 6128-6139. PMID: 24790183, PMCID: PMC4004803, DOI: 10.1523/jneurosci.4941-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAnimalsDrug InteractionsExcitatory Amino Acid AntagonistsFemaleGlutamic AcidHexamethoniumIn Vitro TechniquesLightMaleMiceMice, Inbred C57BLNicotinic AntagonistsPatch-Clamp TechniquesPhotic StimulationPropionatesReceptors, Kainic AcidRetinaRetinal Bipolar CellsSignal TransductionVisual PathwaysConceptsGlutamate releaseBipolar cellsCone bipolar cell typesOFF bipolar cell pathwaysMouse bipolar cellsGanglion cell dendritesKainate-type glutamate receptorsBipolar cell typesLight-evoked responsesBipolar cell pathwaysGanglion cellsOFF layerCell dendritesReceptor mediatesGlutamate receptorsKainate receptorsAMPA receptorsTwo-photon imagingMouse retinaBipolar pathwayIntact circuitsOFF pathwaysElectrophysiological recordingsCircuit mechanismsReceptorsExcitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning
Park SJ, Kim IJ, Looger LL, Demb JB, Borghuis BG. Excitatory Synaptic Inputs to Mouse On-Off Direction-Selective Retinal Ganglion Cells Lack Direction Tuning. Journal Of Neuroscience 2014, 34: 3976-3981. PMID: 24623775, PMCID: PMC3951696, DOI: 10.1523/jneurosci.5017-13.2014.Peer-Reviewed Original ResearchConceptsDirection-selective ganglion cellsOFF direction-selective ganglion cellsStarburst amacrine cellsGlutamate releaseBipolar cellsExcitatory currentsPreferred direction motionBipolar cell typesExcitatory synaptic inputsNull-direction inhibitionGABA-A receptorsWhole-cell recordingsNull-direction motionDSGC dendritesGABA releaseCholinergic inputAmacrine cellsGanglion cellsSynaptic mechanismsSynaptic inputsMammalian retinaMouse retinaIntensity-based glutamate-sensing fluorescent reporterDirection selectivityDirection tuningDevelopmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells
Stafford BK, Park SJ, Wong KY, Demb JB. Developmental Changes in NMDA Receptor Subunit Composition at ON and OFF Bipolar Cell Synapses onto Direction-Selective Retinal Ganglion Cells. Journal Of Neuroscience 2014, 34: 1942-1948. PMID: 24478373, PMCID: PMC3905152, DOI: 10.1523/jneurosci.4461-13.2014.Peer-Reviewed Original ResearchMeSH KeywordsAge FactorsAnimalsAnimals, NewbornExcitatory Amino Acid AgonistsFemaleGene Expression Regulation, DevelopmentalGlutamic AcidGreen Fluorescent ProteinsIn Vitro TechniquesLightMaleMembrane PotentialsMiceMice, Inbred C57BLMice, TransgenicNeurotransmitter AgentsN-MethylaspartatePatch-Clamp TechniquesReceptors, N-Methyl-D-AspartateRetinaRetinal Bipolar CellsRetinal Ganglion CellsSynapsesVisual PathwaysConceptsNMDA-type glutamate receptorsDirection-selective ganglion cellsExtrasynaptic NMDA-type glutamate receptorsNMDAR-mediated responsesBipolar cell synapsesGanglion cellsCell synapsesMature retinaSpecific ganglion cell typesDirection-selective retinal ganglion cellsNMDA receptor subunit compositionActivity-dependent synaptic developmentBipolar cell inputsGanglion cell typesRetinal ganglion cellsFirst postnatal monthReceptor subunit compositionWhole-cell recordingsGlutamatergic synapse formationCell typesGluN2B antagonist ifenprodilReceptor blockadeGlutamate releaseBipolar synapsesGlutamatergic synapses
2013
Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina
Borghuis BG, Marvin JS, Looger LL, Demb JB. Two-Photon Imaging of Nonlinear Glutamate Release Dynamics at Bipolar Cell Synapses in the Mouse Retina. Journal Of Neuroscience 2013, 33: 10972-10985. PMID: 23825403, PMCID: PMC3718381, DOI: 10.1523/jneurosci.1241-13.2013.Peer-Reviewed Original ResearchConceptsInner plexiform layerGanglion cellsPlexiform layerY-type retinal ganglion cellsY-type ganglion cellsPresynaptic bipolar cellsBipolar cell outputBipolar cell synapsesRetinal ganglion cellsWhole-cell recordingsPostsynaptic ganglion cellsIntact neural circuitsReceptive field propertiesPresynaptic release sitesBipolar terminalsGlutamate releaseGlutamate synapsesSynaptic basisCell synapsesBipolar cellsExcitatory currentsSurround inhibitionMouse retinaTwo-photon imagingIndependent release
2008
Functional circuitry of visual adaptation in the retina
Demb JB. Functional circuitry of visual adaptation in the retina. The Journal Of Physiology 2008, 586: 4377-4384. PMID: 18617564, PMCID: PMC2614018, DOI: 10.1113/jphysiol.2008.156638.Peer-Reviewed Original ResearchConceptsPresynaptic bipolar cellsGanglion cellsReceptive field centerBipolar cellsGanglion cell receptive field centersPeripheral receptive fieldsBipolar cell inputsContrast adaptationRetinal ganglion cellsBipolar terminalsGlutamate releaseAmacrine cellsSynaptic outputMammalian retinaContrast stimulationFunctional circuitryCellular mechanismsSpike generationCell inputHyperpolarizationReceptive fieldsRetinaResponse saturationField centerCells
2007
Functional Circuitry for Peripheral Suppression in Mammalian Y-Type Retinal Ganglion Cells
Zaghloul KA, Manookin MB, Borghuis BG, Boahen K, Demb JB. Functional Circuitry for Peripheral Suppression in Mammalian Y-Type Retinal Ganglion Cells. Journal Of Neurophysiology 2007, 97: 4327-4340. PMID: 17460102, DOI: 10.1152/jn.01091.2006.Peer-Reviewed Original ResearchConceptsAmacrine cellsGanglion cellsCenter responseSynaptic layersY-type retinal ganglion cellsHorizontal cellsY-type ganglion cellsSecond synaptic layerGanglion cell receptive fieldsRetinal ganglion cellsGuinea pig retinaStimulus-response curvesRetinal ganglion cell receptive fieldsFirst synaptic layerCurrent-clamp conditionsBipolar terminalsGlutamate releaseCell receptive fieldsPeripheral gratingsPeripheral suppressionIntracellular recordingsPig retinaSubthreshold membrane potentialsExcitatory centerInhibitory conductance
2006
Presynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells
Manookin MB, Demb JB. Presynaptic Mechanism for Slow Contrast Adaptation in Mammalian Retinal Ganglion Cells. Neuron 2006, 50: 453-464. PMID: 16675399, DOI: 10.1016/j.neuron.2006.03.039.Peer-Reviewed Original ResearchMeSH KeywordsAction PotentialsAdaptation, PhysiologicalAnimalsCalcium ChannelsContrast SensitivityGlutamic AcidGuinea PigsOrgan Culture TechniquesPhotic StimulationPresynaptic TerminalsReaction TimeReceptors, GABAReceptors, GlycineReceptors, Metabotropic GlutamateRetinal Bipolar CellsRetinal Ganglion CellsSynaptic TransmissionConceptsPresynaptic bipolar cellsRetinal ganglion cellsGanglion cellsBipolar cellsMammalian retinal ganglion cellsSlow contrast adaptationReduced glutamate releaseIntrinsic cellular propertiesIntrinsic cellular mechanismsCortical adaptationGlutamate releasePresynaptic mechanismsGlycine receptorsVisual neuronsAfterhyperpolarizationExcitatory conductancePotassium conductanceContrast adaptationCellular mechanismsCortical cellsNeuronsCellsCellular propertiesMGluRsSpatial stimuli
2003
Different Circuits for ON and OFF Retinal Ganglion Cells Cause Different Contrast Sensitivities
Zaghloul KA, Boahen K, Demb JB. Different Circuits for ON and OFF Retinal Ganglion Cells Cause Different Contrast Sensitivities. Journal Of Neuroscience 2003, 23: 2645-2654. PMID: 12684450, PMCID: PMC6742092, DOI: 10.1523/jneurosci.23-07-02645.2003.Peer-Reviewed Original ResearchConceptsOFF cellsGanglion cellsSynaptic inputsContrast sensitivityExcitatory conductanceOFF retinal ganglion cellsOFF ganglion cellsBasal glutamate releaseRetinal ganglion cellsBrisk-transient cellsGuinea pig retinaBipolar terminalsPresynaptic circuitsGlutamate releaseIntracellular recordingsON pathwayPig retinaSubthreshold membrane potentialsLight incrementsL-APMembrane potentialCellsMean luminancePathwayRetina