2018
The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development
Tebbenkamp ATN, Varela L, Choi J, Paredes MI, Giani AM, Song JE, Sestan-Pesa M, Franjic D, Sousa AMM, Liu ZW, Li M, Bichsel C, Koch M, Szigeti-Buck K, Liu F, Li Z, Kawasawa YI, Paspalas CD, Mineur YS, Prontera P, Merla G, Picciotto MR, Arnsten AFT, Horvath TL, Sestan N. The 7q11.23 Protein DNAJC30 Interacts with ATP Synthase and Links Mitochondria to Brain Development. Cell 2018, 175: 1088-1104.e23. PMID: 30318146, PMCID: PMC6459420, DOI: 10.1016/j.cell.2018.09.014.Peer-Reviewed Original ResearchConceptsCopy number variationsATP synthase dimersOxidative phosphorylation supercomplexesHuman neurodevelopmental disordersATP synthaseWS pathogenesisGene contributionMitochondrial featuresBrain developmentWilliams syndromeMitochondrial dysfunctionNeocortical pyramidal neuronsNeural phenotypesMitochondriaPyramidal neuronsMachineryMorphological featuresNeurodevelopmental disordersDysfunctionSupercomplexesPhenotypeStriatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons
Li D, Musante V, Zhou W, Picciotto MR, Nairn AC. Striatin-1 is a B subunit of protein phosphatase PP2A that regulates dendritic arborization and spine development in striatal neurons. Journal Of Biological Chemistry 2018, 293: 11179-11194. PMID: 29802198, PMCID: PMC6052221, DOI: 10.1074/jbc.ra117.001519.Peer-Reviewed Original ResearchConceptsSerine/threonine phosphatase PP2AStriatin-interacting phosphataseRNA knockdown approachB subunitSTRIPAK complexPhosphatase PP2AProtein phosphataseMultiprotein complexesKnockdown approachStriatin familyMutant constructsStriatal neuronal culturesPP2ANeuronal developmentPrimary striatal neuronal culturesDendritic phenotypeKnockdown modelSynapse formationSubunitsSpine developmentSelective roleReduced expressionNeuron maturationNeuronal culturesStriatal neurons
2011
Local Application of Neurotrophins Specifies Axons Through Inositol 1,4,5-Trisphosphate, Calcium, and Ca2+/Calmodulin–Dependent Protein Kinases
Nakamuta S, Funahashi Y, Namba T, Arimura N, Picciotto MR, Tokumitsu H, Soderling TR, Sakakibara A, Miyata T, Kamiguchi H, Kaibuchi K. Local Application of Neurotrophins Specifies Axons Through Inositol 1,4,5-Trisphosphate, Calcium, and Ca2+/Calmodulin–Dependent Protein Kinases. Science Signaling 2011, 4: ra76. PMID: 22087032, DOI: 10.1126/scisignal.2002011.Peer-Reviewed Original ResearchConceptsBrain-derived neurotrophic factorNeurotrophin-3Neurotrophin brain-derived neurotrophic factorLocal applicationImmature neuritesCultured hippocampal neuronsAxon specificationSpecific intracellular pathwaysNeurotrophic factorCortical neuronsHippocampal neuronsNeurotrophin receptorParacrine fashionCultured neuronsDependent protein kinase kinaseDependent protein kinaseNeuronsProtein kinase kinaseAxonsIntracellular pathwaysDependent fashionLocal activationNeuritesNeurite outgrowthGrowth cones
2008
Knockout of STriatal enriched protein tyrosine phosphatase in mice results in increased ERK1/2 phosphorylation
Venkitaramani DV, Paul S, Zhang Y, Kurup P, Ding L, Tressler L, Allen M, Sacca R, Picciotto MR, Lombroso PJ. Knockout of STriatal enriched protein tyrosine phosphatase in mice results in increased ERK1/2 phosphorylation. Synapse 2008, 63: 69-81. PMID: 18932218, PMCID: PMC2706508, DOI: 10.1002/syn.20608.Peer-Reviewed Original ResearchConceptsSTEP knockout miceStriatal enriched protein tyrosine phosphataseKnockout miceWild-type miceERK1/2 activityHomozygous knockout miceBrain-specific proteinsExtracellular signal-regulated kinase1/2Wild-type controlsCA2 regionKO miceSTEP protein levelsLateral nucleusCytoarchitectural abnormalitiesSynaptic stimulationCultured neuronsSynaptic plasticityMice resultsHeterozygous miceMiceERK1/2 phosphorylationProtein tyrosine phosphataseProtein levels
2007
Nicotine‐induced phosphorylation of ERK in mouse primary cortical neurons: evidence for involvement of glutamatergic signaling and CaMKII
Steiner RC, Heath CJ, Picciotto MR. Nicotine‐induced phosphorylation of ERK in mouse primary cortical neurons: evidence for involvement of glutamatergic signaling and CaMKII. Journal Of Neurochemistry 2007, 103: 666-678. PMID: 17666046, DOI: 10.1111/j.1471-4159.2007.04799.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCalcium-Calmodulin-Dependent Protein Kinase Type 2Cells, CulturedCerebral CortexCulture MediaDose-Response Relationship, DrugExtracellular Signal-Regulated MAP KinasesFemaleGlutamic AcidIndicators and ReagentsMiceMice, Inbred C57BLMice, KnockoutNeuronsNicotineNicotinic AgonistsPhosphorylationPregnancyReceptors, GlutamateReceptors, NicotinicReverse Transcriptase Polymerase Chain ReactionSignal TransductionSynaptic TransmissionConceptsNicotine-induced ERK phosphorylationExtracellular signal-regulated kinaseERK phosphorylationCAMP-dependent protein kinaseCalmodulin-dependent protein kinase IICalcium/calmodulin-dependent protein kinase IINicotinic acetylcholine receptor inhibitorNicotine-induced phosphorylationSignal-regulated kinaseCortical neuronsProtein kinase IIProtein kinase CMouse primary cortical neuronsKinase II activityAlpha3/beta4Calmodulin-dependent protein kinase II activityGlutamatergic signalingProtein kinaseVoltage-gated sodium channelsKinase IICultured mouse cortical neuronsKinase CCalcium/calmodulin-dependent protein kinase II activityPhosphorylationL-type voltage-gated calcium channelsA Calcium- and Calmodulin-Dependent Kinase Iα/Microtubule Affinity Regulating Kinase 2 Signaling Cascade Mediates Calcium-Dependent Neurite Outgrowth
Uboha NV, Flajolet M, Nairn AC, Picciotto MR. A Calcium- and Calmodulin-Dependent Kinase Iα/Microtubule Affinity Regulating Kinase 2 Signaling Cascade Mediates Calcium-Dependent Neurite Outgrowth. Journal Of Neuroscience 2007, 27: 4413-4423. PMID: 17442826, PMCID: PMC6672303, DOI: 10.1523/jneurosci.0725-07.2007.Peer-Reviewed Original ResearchConceptsKinase domainNeurite outgrowthNeuronal differentiationCalmodulin-dependent kinase ITwo-hybridNeuro-2a cellsPhosphorylation sitesSignal transductionKinase ICaMKIKinase activityMicrotubule affinityKinase 2Calcium-dependent pathwayCritical regulatorIndependent screensPrimary hippocampal neuronsNovel siteCalcium ionophore ionomycinOutgrowthIonophore ionomycinSynaptic plasticityDifferentiationHippocampal neuronsPhysiological effects
2006
Galanin and galanin‐like peptide modulate neurite outgrowth via protein kinase C‐mediated activation of extracellular signal‐related kinase
Hawes JJ, Narasimhaiah R, Picciotto MR. Galanin and galanin‐like peptide modulate neurite outgrowth via protein kinase C‐mediated activation of extracellular signal‐related kinase. European Journal Of Neuroscience 2006, 23: 2937-2946. PMID: 16819983, DOI: 10.1111/j.1460-9568.2006.04828.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlotting, WesternCell DifferentiationCells, CulturedDose-Response Relationship, DrugDrug InteractionsEmbryo, MammalianEnzyme ActivationEnzyme InhibitorsExtracellular Signal-Regulated MAP KinasesGalaninGalanin-Like PeptideNeuritesNeuronsProtein Kinase CRatsReceptors, GalaninReverse Transcriptase Polymerase Chain ReactionRNA, MessengerStem CellsConceptsGalanin-like peptideProtein kinase CNervous systemNeurite outgrowthAdult hippocampal progenitor cellsAbility of galaninKinase CERK phosphorylationHippocampal progenitor cellsNeuronal cell line PC12Central nervous systemModulates Neurite OutgrowthExtracellular signal-related kinase (ERK) phosphorylationConcentration-dependent mannerNerve injuryNeuropeptide galaninNeurotrophic effectsExtracellular signal-related kinaseReceptor subtypesNovel physiological roleAdult brainGalaninCell line PC12Signal-related kinaseProgenitor cellsGalanin attenuates cyclic AMP regulatory element‐binding protein (CREB) phosphorylation induced by chronic morphine and naloxone challenge in Cath.a cells and primary striatal cultures
Hawes JJ, Narasimhaiah R, Picciotto MR. Galanin attenuates cyclic AMP regulatory element‐binding protein (CREB) phosphorylation induced by chronic morphine and naloxone challenge in Cath.a cells and primary striatal cultures. Journal Of Neurochemistry 2006, 96: 1160-1168. PMID: 16417577, DOI: 10.1111/j.1471-4159.2005.03613.x.Peer-Reviewed Original ResearchConceptsOpiate withdrawalAdditional potential therapeutic targetsChronic opiate administrationPotential therapeutic targetPrimary striatal culturesExtracellular signal-regulated kinase 1/2 phosphorylationElement-Binding Protein PhosphorylationCAMP regulatory element-binding proteinKinase 1/2 phosphorylationGalanin treatmentMorphine administrationChronic morphineGalanin receptorsNeuropeptide galaninOpiate administrationNaloxone challengeStriatal neuronsLocus coeruleusSomatic signsNucleus accumbensStriatal culturesRegulatory element-binding proteinTherapeutic targetElement-binding proteinMolecular alterations
2003
Neuroprotection by Nicotine in Mouse Primary Cortical Cultures Involves Activation of Calcineurin and L-Type Calcium Channel Inactivation
Stevens TR, Krueger SR, Fitzsimonds RM, Picciotto MR. Neuroprotection by Nicotine in Mouse Primary Cortical Cultures Involves Activation of Calcineurin and L-Type Calcium Channel Inactivation. Journal Of Neuroscience 2003, 23: 10093-10099. PMID: 14602824, PMCID: PMC6740855, DOI: 10.1523/jneurosci.23-31-10093.2003.Peer-Reviewed Original ResearchMeSH KeywordsAlpha7 Nicotinic Acetylcholine ReceptorAnimalsBungarotoxinsCalcineurinCalcineurin InhibitorsCalcium Channel BlockersCalcium Channels, L-TypeCells, CulturedCerebral CortexGlutamic AcidMiceMice, KnockoutNeuronsNeuroprotective AgentsNeurotoxinsNicotineNifedipineReceptors, NicotinicTacrolimusConceptsPrimary cortical culturesL-type channelsNicotine-mediated neuroprotectionCortical culturesNicotine pretreatmentIntracellular calciumCalcium influxActivation of calcineurinBeta2 subunitAlpha7-containing nAChRsL-type calcium channelsEffects of nicotineAbility of nicotineMouse primary cortical culturesNicotinic acetylcholine receptorsCalcium channel inactivationGlutamate excitotoxicityNeuroprotective effectsRole of calciumCalcineurin antagonistsCortical neuronsNeuronal excitabilityGlutamate applicationNeuroprotectionCalcium channels
1998
Pharmacological characterization of nicotinic receptor-stimulated GABA release from mouse brain synaptosomes.
Lu Y, Grady S, Marks M, Picciotto M, Changeux J, Collins A. Pharmacological characterization of nicotinic receptor-stimulated GABA release from mouse brain synaptosomes. Journal Of Pharmacology And Experimental Therapeutics 1998, 287: 648-57. PMID: 9808692.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidGABA releaseMouse brain synaptosomesBrain synaptosomesNicotinic agonistsNicotine bindingBeta2 null mutant miceNull mutant miceRecent electrophysiological studiesRodent brain tissueConcentration-dependent mannerAgonist inhibitionAlpha-BungarotoxinNeurochemical approachesPharmacological characterizationNM tetrodotoxinElectrophysiological studiesSame agonistBrain regionsBrain tissueMutant miceAgonistsEmax valuesBeta2 subunitMaximal release