2001
In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy
Shen S, Arhin G, Ullu E, Tschudi C. In vivo epitope tagging of Trypanosoma brucei genes using a one step PCR-based strategy. Molecular And Biochemical Parasitology 2001, 113: 171-173. PMID: 11254965, DOI: 10.1016/s0166-6851(00)00383-2.Peer-Reviewed Original Research
2000
Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms
Günzl A, Bindereif A, Ullu E, Tschudi C. Determinants for cap trimethylation of the U2 small nuclear RNA are not conserved between Trypanosoma brucei and higher eukaryotic organisms. Nucleic Acids Research 2000, 28: 3702-3709. PMID: 11000261, PMCID: PMC110770, DOI: 10.1093/nar/28.19.3702.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBinding SitesCell LineConserved SequenceGuanosineMethylationMolecular Sequence DataMutationNucleic Acid ConformationPrecipitin TestsPromoter Regions, GeneticProtein BindingRegulatory Sequences, Nucleic AcidRibonucleoproteins, Small NuclearRNA CapsRNA, ProtozoanRNA, Small NuclearTransfectionTrypanosoma brucei bruceiConceptsCap trimethylationRibonucleoprotein particleEukaryotic organismsU2 snRNATrypanosoma bruceiU2 small nuclear RNA genesCap structureProtist parasite Trypanosoma bruceiSmall nuclear RNA genesCommon proteinsU2 small nuclear RNANuclear RNA genesHigher eukaryotic organismsRNA polymerase IIRNA polymerase IIISmall nuclear RNAMutant U2 snRNAsSet of proteinsParasite Trypanosoma bruceiSpliceosomal snRNPsRNP assemblyNuclear importPolymerase IIRNA genesSequence motifsGenetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA.
Shi H, Djikeng A, Mark T, Wirtz E, Tschudi C, Ullu E. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 2000, 6: 1069-76. PMID: 10917601, PMCID: PMC1369981, DOI: 10.1017/s1355838200000297.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsDNA-Directed RNA PolymerasesDose-Response Relationship, DrugDown-RegulationElectroporationGene ExpressionGenetic EngineeringPhenotypePlasmidsPromoter Regions, GeneticProtein Synthesis InhibitorsProteinsRNA, Double-StrandedTetracyclineTime FactorsTransfectionTrypanosoma brucei bruceiTubulinViral ProteinsConceptsDouble-stranded RNARNA interferenceGenetic interferenceExpression of dsRNAActin mRNAT7 RNA polymerase systemRNA polymerase systemSpecific mRNA degradationStem-loop structureStable cell linesTetracycline-inducible promoterRNAi responseMolecular dissectionMRNA degradationInducible fashionPolymerase systemCellular divisionGene expressionTransfection efficiencyTransient interferenceCell generationCell linesRNAOrganismsInterference
1998
Double-stranded RNA induces mRNA degradation in Trypanosoma brucei
Ngô H, Tschudi C, Gull K, Ullu E. Double-stranded RNA induces mRNA degradation in Trypanosoma brucei. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 14687-14692. PMID: 9843950, PMCID: PMC24510, DOI: 10.1073/pnas.95.25.14687.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsMicroscopy, ElectronRNARNA, MessengerTransfectionTrypanosoma brucei bruceiTubulinConceptsMRNA degradationUntranslated regionFlagellar attachment zoneMRNA 5' untranslated regionAlpha-tubulin synthesisImportant protozoan parasitesCaenorhabditis elegansAlpha-tubulin mRNACellular mRNAsTrypanosome cellsGenetic interferenceCytoskeletal structuresCleavage furrowDsRNA transfectionSame phenotypeCytokinesisComplex cortical structureFlagellar axonemeTubulin mRNAPlasmid vectorProtozoan parasiteVivo expressionAttachment zoneMRNADsRNA