2000
Import of proteins into the trypanosome nucleus and their distribution at karyokinesis
Marchetti M, Tschudi C, Kwon H, Wolin S, Ullu E. Import of proteins into the trypanosome nucleus and their distribution at karyokinesis. Journal Of Cell Science 2000, 113: 899-906. PMID: 10671379, DOI: 10.1242/jcs.113.5.899.Peer-Reviewed Original ResearchConceptsNuclear localization sequenceMonopartite nuclear localization sequenceBipartite nuclear localization sequenceFusion proteinSpecific nuclear localization sequencesImport of proteinsEarly divergent eukaryoteGreen fluorescent proteinDivergent eukaryotesOrganism's proteinsChromosome segregationBeta-galactosidase reporterTrypanosomatid protozoaProtein homologueTrypanosomal proteinsHistone H2BHistone H2B.Localization sequenceNuclear divisionLa proteinC-terminusTrypanosome nucleusMitotic cellsFluorescent proteinATP synthesis
1998
Trypanosome Capping Enzymes Display a Novel Two-Domain Structure
Silva E, Ullu E, Kobayashi R, Tschudi C. Trypanosome Capping Enzymes Display a Novel Two-Domain Structure. Molecular And Cellular Biology 1998, 18: 4612-4619. PMID: 9671471, PMCID: PMC109047, DOI: 10.1128/mcb.18.8.4612.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsBase SequenceBinding SitesCloning, MolecularConserved SequenceCrithidia fasciculataDNA, ProtozoanGuanosine MonophosphateHumansMiceMolecular Sequence DataRecombinant Fusion ProteinsRNA NucleotidyltransferasesSequence Homology, Amino AcidTrypanosoma brucei bruceiConceptsSpliceosomal small nuclear RNAsM7G capCapping enzymeTwo-domain structureG capYeast capping enzymesSmall nuclear RNAPhosphate-binding loopAmino-terminal halfCarboxy-terminal halfGTP-binding proteinsDiphosphate endTrans splicingSignature motifTranscript elongationM7G residueSL sequenceTrypanosomatid protozoaEukaryotic mRNAsLeader RNAGene codesRNA modificationsNuclear RNAOverall identityTrypanosoma brucei