2020
Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma b rucei
Rajan KS, Doniger T, Cohen-Chalamish S, Rengaraj P, Galili B, Aryal S, Unger R, Tschudi C, Michaeli S. Developmentally Regulated Novel Non-coding Anti-sense Regulators of mRNA Translation in Trypanosoma b rucei. IScience 2020, 23: 101780. PMID: 33294788, PMCID: PMC7683347, DOI: 10.1016/j.isci.2020.101780.Peer-Reviewed Original ResearchRNA regulatorsProtein coding genesDistinct mRNA speciesNon-coding RNAsCoding genesPolycistronic transcriptsTranscriptional regulationMRNA translationRRNA locusMRNA functionMammalian hostsMRNA speciesMRNA stabilityGene expressionConcerted actionRegulatorParasitesRNACausative agentRegulationInsectsPolyadenylationSplicingTranslationAdditional layer
2019
The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA
Kolev NG, Rajan KS, Tycowski KT, Toh JY, Shi H, Lei Y, Michaeli S, Tschudi C. The vault RNA of Trypanosoma brucei plays a role in the production of trans-spliced mRNA. Journal Of Biological Chemistry 2019, 294: 15559-15574. PMID: 31439669, PMCID: PMC6816085, DOI: 10.1074/jbc.ra119.008580.Peer-Reviewed Original ResearchConceptsTelomerase-associated protein 1Vault RNAY RNAsRNA quality controlRNA polymerase IIISmall nuclear RNATrans-spliced mRNAPermeabilized cell systemNcRNA repertoireRNP biogenesisMRNA metabolismRNA speciesSequence similarityRNA classesMammalian cellsNuclear RNAPolymerase IIIRo functionTrypanosoma bruceiVtRNAsGene expressionRo proteinBloodstream formsRNACell nuclei
2000
Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA.
Shi H, Djikeng A, Mark T, Wirtz E, Tschudi C, Ullu E. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 2000, 6: 1069-76. PMID: 10917601, PMCID: PMC1369981, DOI: 10.1017/s1355838200000297.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsDNA-Directed RNA PolymerasesDose-Response Relationship, DrugDown-RegulationElectroporationGene ExpressionGenetic EngineeringPhenotypePlasmidsPromoter Regions, GeneticProtein Synthesis InhibitorsProteinsRNA, Double-StrandedTetracyclineTime FactorsTransfectionTrypanosoma brucei bruceiTubulinViral ProteinsConceptsDouble-stranded RNARNA interferenceGenetic interferenceExpression of dsRNAActin mRNAT7 RNA polymerase systemRNA polymerase systemSpecific mRNA degradationStem-loop structureStable cell linesTetracycline-inducible promoterRNAi responseMolecular dissectionMRNA degradationInducible fashionPolymerase systemCellular divisionGene expressionTransfection efficiencyTransient interferenceCell generationCell linesRNAOrganismsInterferenceRNA Metabolism in Trypanosomes: Approaches to Unravel pre-mRNA Signals Required for Intron Removal
Tschudi C, Ullu E. RNA Metabolism in Trypanosomes: Approaches to Unravel pre-mRNA Signals Required for Intron Removal. 2000, 19-42. DOI: 10.1007/978-1-4757-4622-8_2.Peer-Reviewed Original ResearchGene expressionRNA metabolismCap 4 modificationMitochondrial RNA editingPost-transcriptional levelCis-acting signalsPolycistronic transcriptionRNA processingRNA editingIntron removalRNA interferenceTranscriptional levelProcessing machineryTrypanosomesExpressionMetabolismPolyadenylationRNAiMajor determinantTranscriptionMachineryEditingRegulationLikely modulationDiscovery
1995
Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *
Günzl A, Tschudi C, Nakaar V, Ullu E. Accurate Transcription of the Trypanosoma brucei U2 Small Nuclear RNA Gene in a Homologous Extract *. Journal Of Biological Chemistry 1995, 270: 17287-17291. PMID: 7542235, DOI: 10.1074/jbc.270.29.17287.Peer-Reviewed Original ResearchConceptsIntragenic control regionRNA polymerase IIIControl regionPolymerase IIIU2 small nuclear RNA genesTranscription systemSmall nuclear RNA genesGene expressionInsect form trypanosomesNuclear RNA genesU2 snRNA genesSnRNA gene promotersB-box elementsFull transcriptional activitySeries of mutationsSnRNA genesRNA genesInsect formsRNA polymeraseGene promoterTrypanosoma bruceiCell-free extractsTransient expressionBox elementTranscriptional activityAn unusual liaison: Small nuclear and cytoplasmis RNA genes team up with tRNA genes in trypanosomatid protozoa
Nakaar V, Tschudi C, Ullu E. An unusual liaison: Small nuclear and cytoplasmis RNA genes team up with tRNA genes in trypanosomatid protozoa. Trends In Parasitology 1995, 11: 225-228. DOI: 10.1016/0169-4758(95)80085-9.Peer-Reviewed Original ResearchTrypanosomatid protozoaRNA polymerase III transcription unitSequence-specific DNA-binding proteinsCis-acting DNA sequencesBasal transcriptional apparatusDNA-binding proteinsTrans-acting factorsStudy of transcriptionTRNA genesTranscriptional apparatusTranscription unitPromoter structureAccessory factorsRNA polymeraseSmall nuclearDNA sequencesGene teamsCytoplasmic RNAGene expressionEnhancer elementsBiochemical interactionsTransfection technologyRecent insightsProtozoaDiverse set
1994
Trypanosomatid protozoa provide paradigms of eukaryotic biology.
Tschudi C, Ullu E. Trypanosomatid protozoa provide paradigms of eukaryotic biology. Infectious Agents And Disease 1994, 3: 181-6. PMID: 7827786.Peer-Reviewed Original Research