2021
Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma brucei
Toh JY, Nkouawa A, Sánchez SR, Shi H, Kolev NG, Tschudi C. Identification of positive and negative regulators in the stepwise developmental progression towards infectivity in Trypanosoma brucei. Scientific Reports 2021, 11: 5755. PMID: 33707699, PMCID: PMC7952579, DOI: 10.1038/s41598-021-85225-2.Peer-Reviewed Original ResearchConceptsGene expression regulatory networksMetacyclic variant surface glycoproteinSingle RNA-binding proteinExpression regulatory networksRNA-binding proteinTsetse fly vectorBloodstream form trypanosomesAlanine-rich proteinPutative nucleic acidRNA-seq dataSubset of transcriptsCell linesDevelopmental progressionVariant surface glycoproteinMitochondrial genomeOverexpression cell linesFunction phenotypesRegulatory networksProcyclic trypanosomesTrypanosoma bruceiNegative regulatorRich proteinImportant humanMetacyclic parasitesGenes
2000
Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA.
Shi H, Djikeng A, Mark T, Wirtz E, Tschudi C, Ullu E. Genetic interference in Trypanosoma brucei by heritable and inducible double-stranded RNA. RNA 2000, 6: 1069-76. PMID: 10917601, PMCID: PMC1369981, DOI: 10.1017/s1355838200000297.Peer-Reviewed Original ResearchMeSH KeywordsActinsAnimalsDNA-Directed RNA PolymerasesDose-Response Relationship, DrugDown-RegulationElectroporationGene ExpressionGenetic EngineeringPhenotypePlasmidsPromoter Regions, GeneticProtein Synthesis InhibitorsProteinsRNA, Double-StrandedTetracyclineTime FactorsTransfectionTrypanosoma brucei bruceiTubulinViral ProteinsConceptsDouble-stranded RNARNA interferenceGenetic interferenceExpression of dsRNAActin mRNAT7 RNA polymerase systemRNA polymerase systemSpecific mRNA degradationStem-loop structureStable cell linesTetracycline-inducible promoterRNAi responseMolecular dissectionMRNA degradationInducible fashionPolymerase systemCellular divisionGene expressionTransfection efficiencyTransient interferenceCell generationCell linesRNAOrganismsInterference
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