2012
Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations
Jain R, Behrens AJ, Kaever V, Kazmierczak BI. Type IV Pilus Assembly in Pseudomonas aeruginosa over a Broad Range of Cyclic di-GMP Concentrations. Journal Of Bacteriology 2012, 194: 4285-4294. PMID: 22685276, PMCID: PMC3416225, DOI: 10.1128/jb.00803-12.Peer-Reviewed Original ResearchConceptsType IV piliPilus assemblyDiguanylate cyclasesSurface organellesGGDEF/EAL domain proteinsType IV pilus assemblyPolar type IV piliEAL domain proteinsAssembly of piliGMP concentrationSuppressor screenCaulobacter crescentusDiguanylate cyclaseDomain proteinsSuppressor mutationsAdditional regulatorsSuppressor strainsRobust biofilmsDifferent environmental conditionsAssembly defectsNegative regulatorMicrocolony formationSurface piliFimXP. aeruginosa
2009
Pseudomonas aeruginosa OspR is an oxidative stress sensing regulator that affects pigment production, antibiotic resistance and dissemination during infection
Lan L, Murray TS, Kazmierczak BI, He C. Pseudomonas aeruginosa OspR is an oxidative stress sensing regulator that affects pigment production, antibiotic resistance and dissemination during infection. Molecular Microbiology 2009, 75: 76-91. PMID: 19943895, PMCID: PMC2881571, DOI: 10.1111/j.1365-2958.2009.06955.x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SubstitutionAnimalsAnti-Bacterial AgentsBeta-Lactam ResistanceBeta-LactamsFemaleGene DeletionGene Expression Regulation, BacterialGlutathione PeroxidaseHydrogen PeroxideMiceMice, Inbred C57BLModels, BiologicalMutagenesis, Site-DirectedOxidative StressPigments, BiologicalPneumoniaPseudomonas aeruginosaPseudomonas InfectionsQuorum SensingRepressor ProteinsSignal TransductionStress, PhysiologicalTyrosineVirulenceConceptsOxidative stress sensingCys-24Stress sensingPigment productionNull mutant strainOxidative stressSerine substitution mutantsGlobal regulatorPromoter DNASubstitution mutantsAdditional genesInside hostsQuorum sensingCys residuesMutant strainConstitutive expressionMultiple pathwaysRegulatory effectsBeta-lactam resistanceGenesSignificant inductionRegulatorTyrosine metabolismOSPRP. aeruginosa
2007
Pseudomonas aeruginosa Exhibits Sliding Motility in the Absence of Type IV Pili and Flagella
Murray TS, Kazmierczak BI. Pseudomonas aeruginosa Exhibits Sliding Motility in the Absence of Type IV Pili and Flagella. Journal Of Bacteriology 2007, 190: 2700-2708. PMID: 18065549, PMCID: PMC2293233, DOI: 10.1128/jb.01620-07.Peer-Reviewed Original Research
2006
FlhF Is Required for Swimming and Swarming in Pseudomonas aeruginosa
Murray TS, Kazmierczak BI. FlhF Is Required for Swimming and Swarming in Pseudomonas aeruginosa. Journal Of Bacteriology 2006, 188: 6995-7004. PMID: 16980502, PMCID: PMC1595508, DOI: 10.1128/jb.00790-06.Peer-Reviewed Original ResearchConceptsWild-type bacteriaAssembly of flagellaRod-shaped organismExpression of flagellinFlhF proteinFlagellar assemblyFlagellar genesFlagellar poleFlhFFlagellin expressionMonotrichous bacteriaDecreased transcriptionCell surfaceBacteria resultsBacteriaLiquid mediumTranscriptionFlagellaOrganismsProteinDifferent motility patternsAberrant placementPseudomonas aeruginosaMotilityExpression