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Animal Modeling Core

The Animal Modeling Core provides researchers with access to the latest technologies for hematologic studies in animal models. 

The AMC offers expertise, technical assistance, and mice for human-into-mouse xenotransplantation studies. Mouse models include MITRG-SKI (KnockIn) mice that express human cytokines (M-CSF, IL-3, GM-CSF, THPO) and huSIRPα from the endogenous murine loci in the Rag-/- IL-2Rγ -/- background. 

MI(S)TRG mice are maintained as separate MITRG and MITRG-SKI colonies to generate MISTRG mice that are heterozygous for the huSIRPα gene. 

The AMC also offers training and technical assistance in the study of hematopoiesis and benign hematologic questions in mice. 

Through the Yale Hematology Tissue Bank we are also able to offer primary patient samples. 

For more information contact us and refer to the references below. 

To request services from the YCCEH AMC follow the following instructions and complete the YCCEH-AMC agreement. To request samples from the Yale Hematology Tissue Bank follow the instructions and complete the YHTB agreement

We suggest consultation with us prior to submitting an application to ensure ideal study design and to predict feasibility. Please note that certain research may require material transfer agreements which may delay initiation of research. The YCCEH-AMC operates on a fee for service model and all fees reflect the actual expenses of cost recovery in providing services.

MISTRG engrafted with human MDS
Megakarycytes are stained with huCD61 antibody

Frequently Asked Questions

  1. Rongvaux A, Willinger T, Martinek J, Strowig T, Gearty SV, Teichmann LL, et al. Development and function of human innate immune cells in a humanized mouse model. Nat Biotechnol. 2014;32(4):364-72. 
  2. Rongvaux A, Takizawa H, Strowig T, Willinger T, Eynon EE, Flavell RA, et al. Human hemato- lymphoid system mice: current use and future potential for medicine. Annu Rev Immunol. 2013;31:635- 74. 
  3. Willinger T, Rongvaux A, Takizawa H, Yancopoulos GD, Valenzuela DM, Murphy AJ, et al. Human IL-3/GM-CSF knock-in mice support human alveolar macrophage development and human immune responses in the lung. Proc Natl Acad Sci U S A. 2011;108(6):2390-5. 
  4. Willinger T, Rongvaux A, Strowig T, Manz MG, Flavell RA. Improving human hemato-lymphoid- system mice by cytokine knock-in gene replacement. Trends Immunol. 2011;32(7):321-7. 
  5. Strowig T, Rongvaux A, Rathinam C, Takizawa H, Borsotti C, Philbrick W, et al. Transgenic expression of human signal regulatory protein alpha in Rag2-/-{gamma}c-/- mice improves engraftment of human hematopoietic cells in humanized mice. Proc Natl Acad Sci U S A. 2011;108(32):13218-23. 
  6. Rongvaux A, Willinger T, Takizawa H, Rathinam C, Auerbach W, Murphy AJ, et al. Human thrombopoietin knockin mice efficiently support human hematopoiesis in vivo. Proc Natl Acad Sci U S A. 2011;108(6):2378-83. 
  7. Rathinam C, Poueymirou WT, Rojas J, Murphy AJ, Valenzuela DM, Yancopoulos GD, et al. Efficient differentiation and function of human macrophages in humanized CSF-1 mice. Blood. 2011;118(11):3119-28.