FACSorting and Analysis
FACS-based cell sorting is essential in hematopoietic research. The Core will synergize with the existing Yale Flow Cytometry Core to provide YCCEH members with comprehensive cell sorting services of human and mouse hematopoietic cells. In the Yale Flow Cytometry Core facility, 4 full-time personnel are available for performing sorting with four BD Aria machines, a Beckman Coulter MoFlo and a Sony SY3200. Core staff also train and/or assist individuals in performing their own sorts. The Flow Cytometry Core charges based on cost recovery, and costs are kept low due to the high volume of usage.
Complementary to the existing services by the Yale Flow Cytometry Core, the YCCEH Cell Preparation and Analysis Core will provide:
- assistance and advice in experimental design to YCCEH members, such as determining which subpopulation(s) may be optimal for a given experiment, which antibody combinations are most effective, which fluor combinations are best, which collection medium/reagent is most suitable, and whether double sorting may be necessary;
- training on performing population depletion, which is necessary to enrich for low-abundance populations before FACS-sorting; and
- hands-on training for antibody staining of hematopoietic cells.
Training for (2) and (3) will occur once per month, or as determined by the number of requests for training.