2016
Implantable tissue-engineered blood vessels from human induced pluripotent stem cells
Gui L, Dash BC, Luo J, Qin L, Zhao L, Yamamoto K, Hashimoto T, Wu H, Dardik A, Tellides G, Niklason LE, Qyang Y. Implantable tissue-engineered blood vessels from human induced pluripotent stem cells. Biomaterials 2016, 102: 120-129. PMID: 27336184, PMCID: PMC4939127, DOI: 10.1016/j.biomaterials.2016.06.010.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsVascular diseaseBlood vesselsAlpha-smooth muscle actinSmooth muscle myosin heavy chainActive vascular remodelingSmooth muscle cellsMuscle myosin heavy chainTissue-engineered blood vesselsStem cellsAbundant collagenous matrixPluripotent stem cellsInterposition graftAllogeneic graftsVascular remodelingΑ-SMANude ratsMuscle actinMyosin heavy chainClinical useMuscle cellsFunctional vascular smooth muscle cellsPatientsFunctional tissue-engineered blood vesselGraft
2013
Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix
Ghaedi M, Calle EA, Mendez JJ, Gard AL, Balestrini J, Booth A, Bove PF, Gui L, White ES, Niklason LE. Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix. Journal Of Clinical Investigation 2013, 123: 4950-4962. PMID: 24135142, PMCID: PMC3809786, DOI: 10.1172/jci68793.Peer-Reviewed Original ResearchMeSH KeywordsAlveolar Epithelial CellsAnimalsBiomarkersCell AdhesionCell DifferentiationCell ProliferationExtracellular MatrixHumansInduced Pluripotent Stem CellsMiceMucin-1Pulmonary AlveoliPulmonary Surfactant-Associated Protein BPulmonary Surfactant-Associated Protein CRatsTissue EngineeringTissue ScaffoldsConceptsAlveolar epithelial type IILung tissueInduced pluripotent stem cellsHuman induced pluripotent stem cellsWnt/β-catenin inhibitorAcellular lung matrixHuman adult lungRespiratory epithelial cellsΒ-catenin inhibitorType I cellsLung tissue engineeringLung extracellular matrixLung matrixAlveolar epitheliumPulmonary epitheliumAlveolar cellsAdult lungCell therapySurfactant protein CMucin 1Protein CEpithelial cellsProgenitor cellsI cellsSurfactant protein B
2012
Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells
Zhou J, Niklason LE. Microfluidic artificial “vessels” for dynamic mechanical stimulation of mesenchymal stem cells. Integrative Biology 2012, 4: 1487-1497. PMID: 23114826, PMCID: PMC3628532, DOI: 10.1039/c2ib00171c.Peer-Reviewed Original ResearchMeSH KeywordsBeta CateninBiomechanical PhenomenaBlood VesselsCell DifferentiationCells, CulturedEquipment DesignHumansMesenchymal Stem CellsMicrofluidic Analytical TechniquesSignal TransductionSmad ProteinsStress, MechanicalSystems BiologyTissue EngineeringTransforming Growth Factor betaWnt Signaling PathwayConceptsVascular tissue engineeringMicrofluidic channelTissue engineeringDynamic mechanical stimulationHydrodynamic actuationDeformable membraneDevice fatigueStem cell mechanobiologyCyclic strainComplex mechanical stimulationMechanical environmentCell mechanobiologyMesenchymal stem cellsPerformance degradationCyclic stimulationStem cellsBiomechanical conditionsCyclic circumferential strainMicrochip platformSignal transduction cascadeMechanical stimulationVascular cell lineagesVersatile platformEngineeringCanonical Wnt/β-catenin