2024
Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis
Chanduri M, Kumar A, Weiss D, Emuna N, Barsukov I, Shi M, Tanaka K, Wang X, Datye A, Kanyo J, Collin F, Lam T, Schwarz U, Bai S, Nottoli T, Goult B, Humphrey J, Schwartz M. Cellular stiffness sensing through talin 1 in tissue mechanical homeostasis. Science Advances 2024, 10: eadi6286. PMID: 39167642, PMCID: PMC11338229, DOI: 10.1126/sciadv.adi6286.Peer-Reviewed Original ResearchConceptsTissue mechanical homeostasisStiffness sensingExtracellular matrixTalin-1Mechanical homeostasisExtracellular matrix mechanicsIncreased cell spreadingCell spreadingTalinMutationsCellular sensingFibrillar collagenReduced axial stiffnessTissue mechanical propertiesMechanical propertiesAxial stiffnessCompliant substratesHomeostasisRupture pressureArp2/3ARPC5LStiffnessHomeostasis hypothesisResident cellsTissue stiffnessDynamic biaxial loading of vascular smooth muscle cell seeded tissue equivalents
Paukner D, Jennings I, Cyron C, Humphrey J. Dynamic biaxial loading of vascular smooth muscle cell seeded tissue equivalents. Journal Of The Mechanical Behavior Of Biomedical Materials 2024, 157: 106639. PMID: 38970943, DOI: 10.1016/j.jmbbm.2024.106639.Peer-Reviewed Original ResearchCyclic loadingBiaxial loading conditionsPeriod of cyclic loadingStudy cell-matrix interactionsLoad amplitudeUniaxial experimentsDynamic loadingBiaxial forceCruciform samplesLoading conditionsExternal loadMechanical stateBoundary conditionsLoadExtracellular matrixInfluence cell phenotypeMechanical constraintsRange of forcesCell-matrix interactionsCollagen gelsTissue equivalentsForceSynthetic cellsLoad in vivoVascular smooth muscle cells
2017
Comparison of 10 murine models reveals a distinct biomechanical phenotype in thoracic aortic aneurysms
Bellini C, Bersi MR, Caulk AW, Ferruzzi J, Milewicz DM, Ramirez F, Rifkin DB, Tellides G, Yanagisawa H, Humphrey JD. Comparison of 10 murine models reveals a distinct biomechanical phenotype in thoracic aortic aneurysms. Journal Of The Royal Society Interface 2017, 14: 20161036. PMID: 28490606, PMCID: PMC5454287, DOI: 10.1098/rsif.2016.1036.Peer-Reviewed Original ResearchConceptsGenetic mutationsExtracellular matrix proteinsTransmembrane receptorsCytoskeletal proteinsMatrix proteinsWild-type controlsBiomechanical phenotypeDysfunctional mechanosensingExtracellular matrixDiverse mouse modelsSmooth muscle cellsMutationsMuscle cellsProteinAorta of miceMurine modelCellsMechanosensingElastic fiber integrityMouse modelMechanoregulationStructural integrityPhenotypeIntracellularIntegrity
2014
Mechanotransduction and extracellular matrix homeostasis
Humphrey JD, Dufresne ER, Schwartz MA. Mechanotransduction and extracellular matrix homeostasis. Nature Reviews Molecular Cell Biology 2014, 15: 802-812. PMID: 25355505, PMCID: PMC4513363, DOI: 10.1038/nrm3896.Peer-Reviewed Original ResearchDysfunctional Mechanosensing in Aneurysms
Humphrey JD, Milewicz DM, Tellides G, Schwartz MA. Dysfunctional Mechanosensing in Aneurysms. Science 2014, 344: 477-479. PMID: 24786066, PMCID: PMC4360903, DOI: 10.1126/science.1253026.Peer-Reviewed Original Research