2022
Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis
Ortega-Gonzalez P, Taylor G, Jangra RK, Tenorio R, Fernandez de Castro I, Mainou BA, Orchard RC, Wilen CB, Brigleb PH, Sojati J, Chandran K, Sachse M, Risco C, Dermody TS. Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis. PLOS Pathogens 2022, 18: e1010322. PMID: 35263388, PMCID: PMC8906592, DOI: 10.1371/journal.ppat.1010322.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCholesterolEndosomesHomeostasisHumansMammalsNiemann-Pick C1 ProteinReoviridaeReoviridae InfectionsConceptsNiemann-Pick C1Cholesterol homeostasisActive reovirus core particlesRNA interference screenReovirus outer capsidInfectious subvirion particlesPutative host factorsReovirus infectionTreatment of cellsInterference screenMembrane proteinsEndocytic pathwayMammalian orthoreovirusGenome replicationLate endosomesEndosomal membranesPlasma membraneReovirus attachmentEndoplasmic reticulumOuter capsidViral transcriptionTransport activityEndosomesHomeostasisHuman immunodeficiency virus-1
2014
The Major Cellular Sterol Regulatory Pathway Is Required for Andes Virus Infection
Petersen J, Drake MJ, Bruce EA, Riblett AM, Didigu CA, Wilen CB, Malani N, Male F, Lee FH, Bushman FD, Cherry S, Doms RW, Bates P, Briley K. The Major Cellular Sterol Regulatory Pathway Is Required for Andes Virus Infection. PLOS Pathogens 2014, 10: e1003911. PMID: 24516383, PMCID: PMC3916400, DOI: 10.1371/journal.ppat.1003911.Peer-Reviewed Original ResearchConceptsRegulatory pathwaysHuman haploid cellsParallel genetic screensGenetic screenPathogenic New World hantavirusesGenomic screenAndes virusHaploid cellsGene disruptionRNA interferenceANDV entryDeficient cellsCellular requirementsCellular cholesterolRNA virusesANDV infectionLarge familyPharmacologic inhibitionWorldwide distributionAndes Virus InfectionNew World hantavirusesVirus bindingPathwayHost factorsSterol synthesis