2021
Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein
Hsu JC, Laurent-Rolle M, Pawlak JB, Wilen CB, Cresswell P. Translational shutdown and evasion of the innate immune response by SARS-CoV-2 NSP14 protein. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2101161118. PMID: 34045361, PMCID: PMC8214666, DOI: 10.1073/pnas.2101161118.Peer-Reviewed Original ResearchConceptsSARS-CoV-2Interferon-stimulated genesImmune responseSevere acute respiratory syndrome coronavirus 2Acute respiratory syndrome coronavirus 2Host protein synthesisRespiratory syndrome coronavirus 2Syndrome coronavirus 2Innate immune responseUnprecedented global health crisisCoronavirus 2N7-methyltransferase activityOngoing COVID-19 pandemicHuman coronavirusesTranslational shutdownVirus replicationNsp14 proteinGlobal health crisisProtein synthesisInhibition activityCausative agentCOVID-19COVID-19 pandemicSARS-CoV-2 nsp14Dependent induction
2019
Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation
Hosmillo M, Lu J, McAllaster MR, Eaglesham JB, Wang X, Emmott E, Domingues P, Chaudhry Y, Fitzmaurice TJ, Tung MK, Panas MD, McInerney G, Locker N, Wilen CB, Goodfellow IG. Noroviruses subvert the core stress granule component G3BP1 to promote viral VPg-dependent translation. ELife 2019, 8: e46681. PMID: 31403400, PMCID: PMC6739877, DOI: 10.7554/elife.46681.Peer-Reviewed Original ResearchConceptsViral positive-sense RNAFirst host factorHost factorsPositive-sense RNAPro-viral activityPositive-sense RNA virusesSense RNA virusesG3BP1 functionsRibosome recruitmentTranslation complexesTranslation initiationCRISPR screensProteomic analysisMurine norovirus infectionReplication complexSense RNANovel functionViral translationRNA virusesG3BP1Data uncoversNorovirus replicationLife cycleVPgGenusMouse Norovirus Infection Arrests Host Cell Translation Uncoupled from the Stress Granule-PKR-eIF2α Axis
Fritzlar S, Aktepe TE, Chao YW, Kenney ND, McAllaster MR, Wilen CB, White PA, Mackenzie JM. Mouse Norovirus Infection Arrests Host Cell Translation Uncoupled from the Stress Granule-PKR-eIF2α Axis. MBio 2019, 10: 10.1128/mbio.00960-19. PMID: 31213553, PMCID: PMC6581855, DOI: 10.1128/mbio.00960-19.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCaliciviridae InfectionsCytoplasmic GranulesDNA HelicasesEIF-2 KinaseEukaryotic Initiation Factor-2Host-Pathogen InteractionsImmune EvasionImmunity, InnateMicePhosphorylationPoly-ADP-Ribose Binding ProteinsProtein BiosynthesisRNA HelicasesRNA Recognition Motif ProteinsViral ProteinsVirus ReplicationConceptsIntegrated stress responseProtein kinase RStress granulesProtein translationCellular homeostasisProtein G3BP1Host translationCytoplasmic RNA granulesMNV replication complexHijack host machineryPhosphorylation of eIF2αHost protein translationCellular response systemsHost cell translationEukaryotic initiation factorMNV infectionPhosphorylated eukaryotic initiation factorRNA granulesTranslational controlSG formationInitiation factorsSG nucleationTranslational arrestHost machineryVirus replication