2022
Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis
Ortega-Gonzalez P, Taylor G, Jangra RK, Tenorio R, Fernandez de Castro I, Mainou BA, Orchard RC, Wilen CB, Brigleb PH, Sojati J, Chandran K, Sachse M, Risco C, Dermody TS. Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis. PLOS Pathogens 2022, 18: e1010322. PMID: 35263388, PMCID: PMC8906592, DOI: 10.1371/journal.ppat.1010322.Peer-Reviewed Original ResearchConceptsNiemann-Pick C1Cholesterol homeostasisActive reovirus core particlesRNA interference screenReovirus outer capsidInfectious subvirion particlesPutative host factorsReovirus infectionTreatment of cellsInterference screenMembrane proteinsEndocytic pathwayMammalian orthoreovirusGenome replicationLate endosomesEndosomal membranesPlasma membraneReovirus attachmentEndoplasmic reticulumOuter capsidViral transcriptionTransport activityEndosomesHomeostasisHuman immunodeficiency virus-1
2020
Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase and Solute Carrier Family 35 Member A1 Are Required for Reovirus Binding and Infection
Urbanek K, Sutherland DM, Orchard RC, Wilen CB, Knowlton JJ, Aravamudhan P, Taylor GM, Virgin HW, Dermody TS. Cytidine Monophosphate N-Acetylneuraminic Acid Synthetase and Solute Carrier Family 35 Member A1 Are Required for Reovirus Binding and Infection. Journal Of Virology 2020, 95: 10.1128/jvi.01571-20. PMID: 33087464, PMCID: PMC7944449, DOI: 10.1128/jvi.01571-20.Peer-Reviewed Original ResearchConceptsSialic acid expressionMicroglial cellsCell surface expressionReovirus-induced cell deathReovirus infectionSialic acidMurine microglial BV2 cellsReovirus-induced diseaseMember A1Microglial BV2 cellsSurface expressionMurine microglial cellsCell deathReovirus bindingBV2 cellsViral tropismInfectionHost genesLow-level bindingCell surface receptorsHost factorsCell surfaceReceptorsSialic acid synthesisSurface receptors