1995
CD4 and CD45 regulate qualitatively distinct patterns of calcium mobilization in individual CD4+ T cells
Leitenberg D, Constant S, Lu D, Smith B, Bottomly K. CD4 and CD45 regulate qualitatively distinct patterns of calcium mobilization in individual CD4+ T cells. European Journal Of Immunology 1995, 25: 2445-2451. PMID: 7589109, DOI: 10.1002/eji.1830250906.Peer-Reviewed Original ResearchConceptsIntracellular calcium concentrationCalcium mobilizationSustained increaseT cellsCell activationCalcium concentrationCross-linking CD4Antigen-presenting cellsIntracellular calcium mobilizationT cell interactionsT cell activationT cell receptor complexT cell receptor signalingDistinct patternsCell receptor signalingIndividual CD4Cell receptor complexCD4Extracellular calciumCD3Specific antigenCross-linking antibodiesCalcium spikesCalcium fluxReceptor signaling
1989
Vascular endothelial cells enhance T cell responses by markedly augmenting IL-2 concentrations
Guinan E, Smith B, Doukas J, Miller R, Pober J. Vascular endothelial cells enhance T cell responses by markedly augmenting IL-2 concentrations. Cellular Immunology 1989, 118: 166-177. PMID: 2562928, DOI: 10.1016/0008-8749(89)90366-3.Peer-Reviewed Original ResearchConceptsPeripheral blood mononuclear cellsIL-2 concentrationsPolyclonal mitogen phytohemagglutininT cell responsesEndothelial cellsBlood monocytesUnfractionated peripheral blood mononuclear cellsCell responsesPrimary allogeneic responseSuboptimal PHA concentrationsExogenous IL-2Blood mononuclear cellsT cell populationsRecombinant IL-1Cultured human endothelial cellsDermal fibroblastsIL-2 synthesisVascular endothelial cellsQuantity of antigenHuman endothelial cellsAllogeneic responseMitogen phytohemagglutininMononuclear cellsIL-2Presence of EC
1988
REGULATION OF CYTOTOXIC T LYMPHOCYTE-MEDIATED GRAFT REJECTION FOLLOWING BONE MARROW
Bierer B, Emerson S, Antin J, Maziarz R, Rappeport J, Smith B, Burakoff A. REGULATION OF CYTOTOXIC T LYMPHOCYTE-MEDIATED GRAFT REJECTION FOLLOWING BONE MARROW. Transplantation 1988, 46: 835-839. PMID: 3061078, DOI: 10.1097/00007890-198812000-00009.Peer-Reviewed Original ResearchConceptsGraft rejectionHuman allogeneic bone marrow transplantationT cell-depleted marrowAllogeneic bone marrow transplantationClass I major histocompatibility complex antigensMajor histocompatibility complex antigensLymphocyte function-associated antigen-1Bone marrow transplantationFunction-associated antigen-1Cytotoxic T lymphocytesAnti-CD3 mAbHistocompatibility complex antigensBone marrow progenitor cellsMHC class IMarrow progenitor cellsNumber of mAbsBMT patientsCTL activityEffector cellsMarrow transplantationRecipient originT cellsT lymphocytesComplex antigensBone marrowPhenotypic and functional characterization of human cytolytic T cells lacking expression of CD5.
Bierer BE, Nishimura Y, Burakoff SJ, Smith BR. Phenotypic and functional characterization of human cytolytic T cells lacking expression of CD5. Journal Of Clinical Investigation 1988, 81: 1390-1397. PMID: 2452832, PMCID: PMC442569, DOI: 10.1172/jci113468.Peer-Reviewed Original ResearchConceptsAnti-CD3 mAbNormal cytolytic activityCytolytic activityHuman cytolytic T cellsNormal individualsLymphocyte function-associated antigen-3Blood mononuclear cellsImmunofluorescence flow cytometryCytolytic T cellsExpression of CD5T lymphocyte clonesT cell activationSurface CD3Appropriate target cellsMononuclear cellsNormal CD5CD5 antigenT cellsIntracellular calciumLymphocyte clonesCD5Cell activationCell membrane glycoproteinAntigen 3Lymphoid phenotype
1987
CTL adhesion and antigen recognition are discrete steps in the human CTL-target cell interaction.
Mentzer SJ, Smith BR, Barbosa JA, Crimmins MA, Herrmann SH, Burakoff SJ. CTL adhesion and antigen recognition are discrete steps in the human CTL-target cell interaction. The Journal Of Immunology 1987, 138: 1325-30. PMID: 3543126, DOI: 10.4049/jimmunol.138.5.1325.Peer-Reviewed Original ResearchConceptsHLA-A2CTL clonesHLA-B7Target cellsAntigen recognitionHuman CTL clonesTarget cell lysisCalcium-sensitive dye indo-1Cytolytic T lymphocytesCTL-target interactionDye indo-1CTL-target cell interactionAntigen-specific receptorsT cell receptorCell surface moleculesCTL activationT lymphocytesHuman CTLIndo-1LFA-1Free calciumCell receptorFlow cytometrySurface moleculesCell interactions
1986
FUNCTIONAL ASSESSMENT OF T CELL DEPLETION FROM BONE MARROW PRIOR TO THERAPEUTIC TRANSPLANTATION USING LIMITING DILUTION CULTURE METHODS
Rozans M, Smith B, Emerson S, Crimmins M, Laurent G, Reichert T, Burakoff S, Miller R. FUNCTIONAL ASSESSMENT OF T CELL DEPLETION FROM BONE MARROW PRIOR TO THERAPEUTIC TRANSPLANTATION USING LIMITING DILUTION CULTURE METHODS. Transplantation 1986, 42: 380-386. PMID: 3094209, DOI: 10.1097/00007890-198610000-00010.Peer-Reviewed Original ResearchConceptsT-cell depletionT cellsComplement-mediated depletionCell depletionFACS analysisIL-2Functional impairmentT-lymphocyte depletionT-cell contaminationBone marrow inoculumT cell setsComplement-mediated lysisDilution methodFlow cytometric methodLymphocyte depletionComplement treatmentMarrow inoculumCytotoxic effectorsInterleukin-2Functional assessmentT101 immunotoxinCulture methodCytometric methodCell contaminationEventual death
1981
Increase of surface Ia-like antigen expression on human monocytes independent of antigenic stimuli.
Smith BR, Ault KA. Increase of surface Ia-like antigen expression on human monocytes independent of antigenic stimuli. The Journal Of Immunology 1981, 127: 2020-7. PMID: 6170685, DOI: 10.4049/jimmunol.127.5.2020.Peer-Reviewed Original Research