2025
Molecular Components of Vesicle Cycling at the Rod Photoreceptor Ribbon Synapse
Hanke-Gogokhia C, Zapadka T, Finkelstein S, Arshavsky V, Demb J. Molecular Components of Vesicle Cycling at the Rod Photoreceptor Ribbon Synapse. Advances In Experimental Medicine And Biology 2025, 1468: 325-330. PMID: 39930217, DOI: 10.1007/978-3-031-76550-6_54.Peer-Reviewed Original ResearchConceptsSynaptic vesicle exocytosisSynaptic vesicle recyclingPhotoreceptor ribbon synapseVesicle exocytosisVesicle recyclingVesicle cycleVesicle releaseRibbon synapseProtein synthesisProperties of synaptic transmissionMolecular componentsMouse rodsSynaptic terminalsRod cellsProteinVesiclesRod photoreceptorsDim lightSynaptic transmissionInner segmentsCellsExocytosisEndocytosisOuter segmentsEnergy production
2024
Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis
Kural M, Djakbarova U, Cakir B, Tanaka Y, Chan E, Arteaga Muniz V, Madraki Y, Qian H, Park J, Sewanan L, Park I, Niklason L, Kural C. Mechano-inhibition of endocytosis sensitizes cancer cells to Fas-induced Apoptosis. Cell Death & Disease 2024, 15: 440. PMID: 38909035, PMCID: PMC11193792, DOI: 10.1038/s41419-024-06822-3.Peer-Reviewed Original ResearchConceptsFas-induced apoptosisCell surface Fas expressionDeath receptor FasInhibition of endocytosisSurface Fas expressionPlasma membrane tensionCancer cell apoptosisEndocytosis dynamicsApoptotic signalingReceptor FasGlioblastoma cell growthFas expressionPlasma membraneCell growthEndocytosisXenograft mouse modelSoluble FasLCell apoptosisFasApoptosisRho-kinase inhibitorCancer cellsMembrane tensionNonmalignant cellsInduce tumor regression
2023
Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors
Ren Y, Yang J, Fujita B, Jin H, Zhang Y, Berro J. Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors. Science Advances 2023, 9: eadi1535. PMID: 37831774, PMCID: PMC10575576, DOI: 10.1126/sciadv.adi1535.Peer-Reviewed Original ResearchConceptsActin cytoskeletonPlasma membraneHuntingtin Interacting Protein 1Clathrin-mediated endocytosisCountless cellular processesEndocytic machineryCellular processesClathrin latticesProtein condensationCytoskeletonEnd4pProtein 1Membrane deformationPiconewton forcesEndocytosisVivo force measurementsMembranePiconewtonsClathrinMachineryProteinCoatMolecular scaleMAD2-Dependent Insulin Receptor Endocytosis Regulates Metabolic Homeostasis.
Park J, Hall C, Hubbard B, LaMoia T, Gaspar R, Nasiri A, Li F, Zhang H, Kim J, Haeusler R, Accili D, Shulman G, Yu H, Choi E. MAD2-Dependent Insulin Receptor Endocytosis Regulates Metabolic Homeostasis. Diabetes 2023, 72: 1781-1794. PMID: 37725942, PMCID: PMC10658066, DOI: 10.2337/db23-0314.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsEndocytosisGlucoseHomeostasisInsulinInsulin ResistanceLiverMad2 ProteinsMaleMiceReceptor, InsulinConceptsIR endocytosisInsulin receptor endocytosisCell division regulatorsInsulin receptorProlongs insulin actionReceptor endocytosisTranscriptomic profilesInsulin stimulationEndocytosisMetabolic homeostasisCell surfaceGenetic ablationMetabolic functionsInsulin actionP31cometMad2BubR1DisruptionSignalingRegulatorHomeostasisAdipose tissueInteractionHepatic fat accumulationMetabolismα-Synuclein colocalizes with AP180 and affects the size of clathrin lattices
Vargas K, Colosi P, Girardi E, Park J, Harmon L, Chandra S. α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices. Journal Of Biological Chemistry 2023, 299: 105091. PMID: 37516240, PMCID: PMC10470054, DOI: 10.1016/j.jbc.2023.105091.Peer-Reviewed Original ResearchConceptsClathrin punctaClathrin assemblyEndocytic accessory proteinsΑ-synucleinPresynaptic membraneSynaptic vesicle cyclingImmuno-electron microscopyClathrin structuresAccessory proteinsClathrin latticesMembrane curvatureVesicle cyclingCell membranePresynaptic proteinsLipid monolayer systemProteinΓ-synucleinMembranePunctaAssemblyRelocalizesClathrinColocalizesVesicle sizeDeletionInsights of Endocytosis Signaling in Health and Disease
Pathak C, Vaidya F, Waghela B, Jaiswara P, Gupta V, Kumar A, Rajendran B, Ranjan K. Insights of Endocytosis Signaling in Health and Disease. International Journal Of Molecular Sciences 2023, 24: 2971. PMID: 36769293, PMCID: PMC9918140, DOI: 10.3390/ijms24032971.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportCaveolaeCell MembraneEndocytosisHumansMammalsSignal TransductionConceptsEndocytic machineryPlasma membraneCellular defenseFundamental cellular machinerySignal transduction modulesDifferent cellular compartmentsVital physiological processesProcess of endocytosisEndocytic proteinsIndependent endocytosisEndocytosis machineryTransduction modulesEukaryotic cellsCaveolar pathwayCellular machineryMammalian cellsEndocytic pathwayCell divisionCellular compartmentsComplex proteinsFunctional characterizationPhysiological processesMachinery resultsHuman diseasesEndocytosisUse of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis
Huet-Calderwood C, Rivera-Molina F, Toomre D, Calderwood D. Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis. Methods In Molecular Biology 2023, 2608: 17-38. PMID: 36653699, PMCID: PMC9999384, DOI: 10.1007/978-1-0716-2887-4_2.ChaptersConceptsΒ1 integrinTotal internal reflection fluorescence microscopyNormal cell adhesionIntegrin adhesion receptorsReflection fluorescence microscopyAdhesion receptorsCell adhesionEndocytosisFluorescence microscopyExocytosisIntegrinsCellsHaloTagPHluorinIntracellular labelingEctoPhotobleachingTagsReceptorsChaseFluorescentAdhesionLabelingMigration
2022
Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9
Yang S, Park D, Manning L, Hill SE, Cao M, Xuan Z, Gonzalez I, Dong Y, Clark B, Shao L, Okeke I, Almoril-Porras A, Bai J, De Camilli P, Colón-Ramos DA. Presynaptic autophagy is coupled to the synaptic vesicle cycle via ATG-9. Neuron 2022, 110: 824-840.e10. PMID: 35065714, PMCID: PMC9017068, DOI: 10.1016/j.neuron.2021.12.031.Peer-Reviewed Original ResearchConceptsSynaptic vesicle cycleVesicle cyclePresynaptic autophagyAutophagosome biogenesisATG-9Only transmembrane proteinTrans-Golgi networkCellular degradation pathwayPresynaptic sitesActivity-dependent mannerTransmembrane proteinSynaptojanin 1Synaptic fociBiogenesisAutophagyNeuronal healthDegradation pathwayTraffickingPathwayParkinson's diseaseSynaptic activityNeuronal activityElegansSitesEndocytosisExtracellular Vesicles Mediate the Intercellular Exchange of Nanoparticles
Wu X, Tang T, Wei Y, Cummins K, Wood D, Pang H. Extracellular Vesicles Mediate the Intercellular Exchange of Nanoparticles. Advanced Science 2022, 9: 2102441. PMID: 35243822, PMCID: PMC8895114, DOI: 10.1002/advs.202102441.Peer-Reviewed Original ResearchConceptsIntercellular exchangeExtracellular vesiclesComplex tissuesImportance of EVsCell-cell contactTranscellular transport routeDirect cell-cell contactSubcellular transportNP endocytosisExocytosisAccumulative evidenceVesiclesSame receptorCrucial rolePotential mechanismsCellsTransport routesEndocytosisTissueSignificant fractionTransportVitroAssaysVivoReceptors
2021
MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells
Jeong J, Shin JH, Li W, Hong JY, Lim J, Hwang JY, Chung JJ, Yan Q, Liu Y, Choi J, Wysolmerski J. MAL2 mediates the formation of stable HER2 signaling complexes within lipid raft-rich membrane protrusions in breast cancer cells. Cell Reports 2021, 37: 110160. PMID: 34965434, PMCID: PMC8762588, DOI: 10.1016/j.celrep.2021.110160.Peer-Reviewed Original ResearchMeSH KeywordsAntineoplastic Agents, ImmunologicalBreast NeoplasmsCell ProliferationCytoskeletal ProteinsDrug Resistance, NeoplasmEndocytosisFemaleHumansMembrane MicrodomainsMyelin and Lymphocyte-Associated Proteolipid ProteinsPhosphoproteinsPlasma Membrane Calcium-Transporting ATPasesReceptor, ErbB-2Sodium-Hydrogen ExchangersTrastuzumabTumor Cells, CulturedConceptsLipid raft formationBreast cancer cellsLipid raftsLipid raft resident proteinsCancer cellsRaft formationRaft-resident proteinsProximity ligation assayProtein complexesMembrane protrusionsProtein interactionsPlasma membraneLigation assayMAL2Membrane stabilityStructural organizationPotential therapeutic targetPhysical interactionMembrane retentionProteinRaftsTherapeutic targetCellsIntracellular calcium concentrationLow intracellular calcium concentrationMicroglia regulate brain Progranulin levels through the endocytosis-lysosomal pathway
Dong T, Tejwani L, Jung Y, Kokubu H, Luttik K, Driessen TM, Lim J. Microglia regulate brain Progranulin levels through the endocytosis-lysosomal pathway. JCI Insight 2021, 6: e136147. PMID: 34618685, PMCID: PMC8663778, DOI: 10.1172/jci.insight.136147.Peer-Reviewed Original ResearchConceptsPGRN levelsNovel potential therapeutic targetFrontotemporal lobar degenerationPotential therapeutic targetNeuronal ceroid lipofuscinosisPGRN deficiencyPGRN expressionLysosomal pathwayProgranulin levelsPathological changesHaploinsufficient miceTherapeutic targetMicrogliaNeuropathological phenotypeAlzheimer's diseaseProgranulinCeroid lipofuscinosisGlycoprotein progranulinNeurodegenerative diseasesDiseaseMiceGenetic alterationsNemo-like kinaseGenetic interaction studiesGenetic variantsInhibition of endocytosis of porcine reproductive and respiratory syndrome virus by rottlerin and its potential prophylactic administration in piglets
Kang Y, Oh C, Ahn S, Choi J, Choi H, Lee S, Choi I, Song C, Lee J, Park S. Inhibition of endocytosis of porcine reproductive and respiratory syndrome virus by rottlerin and its potential prophylactic administration in piglets. Antiviral Research 2021, 195: 105191. PMID: 34678331, DOI: 10.1016/j.antiviral.2021.105191.Peer-Reviewed Original ResearchConceptsAnti-PRRSV effectPRRSV infectionBlood viral loadRespiratory syndrome virusInterstitial pneumoniaProphylactic administrationClinical scoresViral loadProphylactic treatmentAntiviral effectAntiviral agentsPRRSV internalizationViral replicationUntreated pigsProtein kinase CTreatmentΔ phosphorylationEntry pathwayInfectionInhibition of endocytosisRottlerinKinase CLimited studiesVivoPigsEndocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β‑Hairpin Antimicrobial Peptides
Buri M, Sperandio L, de Souza K, Antunes F, Rezende M, Melo C, Pinhal M, Barros C, Fernig D, Yates E, Ide J, Smaili S, Riske K, Nader H, dos Santos Tersariol I, Lima M, Judice W, Miranda A, Paredes-Gamero E. Endocytosis and the Participation of Glycosaminoglycans Are Important to the Mechanism of Cell Death Induced by β‑Hairpin Antimicrobial Peptides. ACS Applied Bio Materials 2021, 4: 6488-6501. PMID: 35006908, DOI: 10.1021/acsabm.1c00390.Peer-Reviewed Original ResearchConceptsCell deathAntimicrobial peptidesSecondary structure homologyControl cell deathΒ-hairpin antimicrobial peptidesCommon mechanismCytotoxicity of AMPsStructure homologyMembrane permeabilizationGene expressionMitochondrial potentialCytotoxic modeEndocytosisCell membraneCell tumor modelΒ-hairpin peptidesAMP stimulationPeptidesDynaminHomologyPermeabilizationGlycosaminoglycansMechanismProteinCytotoxicityA kinetic view of clathrin assembly and endocytic cargo sorting
Wu M, Wu X. A kinetic view of clathrin assembly and endocytic cargo sorting. Current Opinion In Cell Biology 2021, 71: 130-138. PMID: 33865229, DOI: 10.1016/j.ceb.2021.02.010.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsAltered endocytosis in cellular senescence
Shin EY, Soung NK, Schwartz MA, Kim EG. Altered endocytosis in cellular senescence. Ageing Research Reviews 2021, 68: 101332. PMID: 33753287, PMCID: PMC8131247, DOI: 10.1016/j.arr.2021.101332.Peer-Reviewed Original ResearchPresynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles
Wu XS, Subramanian S, Zhang Y, Shi B, Xia J, Li T, Guo X, El-Hassar L, Szigeti-Buck K, Henao-Mejia J, Flavell RA, Horvath TL, Jonas EA, Kaczmarek LK, Wu LG. Presynaptic Kv3 channels are required for fast and slow endocytosis of synaptic vesicles. Neuron 2021, 109: 938-946.e5. PMID: 33508244, PMCID: PMC7979485, DOI: 10.1016/j.neuron.2021.01.006.Peer-Reviewed Original ResearchConceptsSlow endocytosisVesicle mobilizationF-actin cytoskeletonChannel mutationsPotassium channelsKv3.3 proteinsInhibits endocytosisRapid endocytosisNovel functionF-actinEndocytosisCrucial functionSynaptic vesiclesFamily channelsSynaptic transmissionDiscovery decadesMembrane potentialNeurotransmitter releaseDiverse neurological disordersIon conductanceMutationsReleasable poolMouse nerve terminalsPotassium channel mutationsPathological effectsCooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells
Park D, Wu Y, Lee SE, Kim G, Jeong S, Milovanovic D, De Camilli P, Chang S. Cooperative function of synaptophysin and synapsin in the generation of synaptic vesicle-like clusters in non-neuronal cells. Nature Communications 2021, 12: 263. PMID: 33431828, PMCID: PMC7801664, DOI: 10.1038/s41467-020-20462-z.Peer-Reviewed Original ResearchConceptsNon-neuronal cellsSV clustersSynaptic vesiclesSmall synaptic-like microvesiclesSV membrane proteinsSynaptic-like microvesiclesSV proteinsDiffuse cytosolic distributionMembrane proteinsReconstitution systemCytosolic distributionCooperative functionSuch vesiclesMechanistic insightsLiquid-like propertiesPowerful modelPhysiological formationProteinSynapsinVesiclesCellsSynaptic transmissionAssembly of structuresDefining featureLiquid condensate
2020
BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells
Tao B, Kraehling JR, Ghaffari S, Ramirez C, Lee S, Fowler JW, Lee WL, Fernandez-Hernando C, Eichmann A, Sessa WC. BMP-9 and LDL crosstalk regulates ALK-1 endocytosis and LDL transcytosis in endothelial cells. Journal Of Biological Chemistry 2020, 295: 18179-18188. PMID: 33097593, PMCID: PMC7939458, DOI: 10.1074/jbc.ra120.015680.Peer-Reviewed Original ResearchConceptsBone morphogenetic protein 9Dynamin 2Caveolin-1LDL transcytosisGene expressionActivin-like kinase 1Endothelial cellsEndothelial cell quiescenceCell surface levelsSmad1/5 phosphorylationExtensive endocytosisUnbiased screenProtein 9Cell quiescenceKinase 1ALK-1BMP-9 levelsEssential roleEndocytosisEndothelial homeostasisHeavy chainSignalingInternalizationTranscytosisCellsDynamin 1 is important for microtubule organization and stabilization in glomerular podocytes
La TM, Tachibana H, Li S, Abe T, Seiriki S, Nagaoka H, Takashima E, Takeda T, Ogawa D, Makino S, Asanuma K, Watanabe M, Tian X, Ishibe S, Sakane A, Sasaki T, Wada J, Takei K, Yamada H. Dynamin 1 is important for microtubule organization and stabilization in glomerular podocytes. The FASEB Journal 2020, 34: 16449-16463. PMID: 33070431, DOI: 10.1096/fj.202001240rr.Peer-Reviewed Original ResearchConceptsDynamin 1Microtubule organizationMouse podocytesΑ-tubulinMicrotubule bundlesGlomerular podocytesEndocytic proteinsMicrotubule bundle formationStabilization of microtubulesAcetylated α-tubulinVesicle formationNocodazole resistanceImmunoelectron microscopyMicrotubulesPhysiological significanceBundle formationRat podocytesIdentification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles.
Groener M, Wang Y, Cross E, Tian X, Ebenezer K, Baik E, Pedigo C, Schiffer M, Inoue K, Ishibe S. Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles. Kidney360 2020, 1: 480-490. PMID: 35368594, PMCID: PMC8809311, DOI: 10.34067/kid.0000212020.Peer-Reviewed Original ResearchConceptsClathrin-coated vesiclesCargo proteinsProteomic analysisPodocyte healthPrimary podocyte cultureTransferrin receptor protein 1Endocytic traffickingSignificant proteinsLiquid chromatography-mass spectrometryPodocyte culturesHigh abundanceGenetic ablationSmall vesiclesCCV fractionsProteinProtein 1Protein markersClathrinVesiclesMultiple protein markersDiphtheria toxinFundamental roleMouse kidneyImmunofluorescence stainingCargo
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