2023
Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection
Wei J, Patil A, Collings C, Alfajaro M, Liang Y, Cai W, Strine M, Filler R, DeWeirdt P, Hanna R, Menasche B, Ökten A, Peña-Hernández M, Klein J, McNamara A, Rosales R, McGovern B, Luis Rodriguez M, García-Sastre A, White K, Qin Y, Doench J, Yan Q, Iwasaki A, Zwaka T, Qi J, Kadoch C, Wilen C. Pharmacological disruption of mSWI/SNF complex activity restricts SARS-CoV-2 infection. Nature Genetics 2023, 55: 471-483. PMID: 36894709, PMCID: PMC10011139, DOI: 10.1038/s41588-023-01307-z.Peer-Reviewed Original ResearchConceptsMSWI/SNF complexesAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionHost-directed therapeutic targetSyndrome coronavirus 2 infectionSARS-CoV-2 infectionSWItch/Sucrose Non-Fermentable (SWI/SNF) chromatinSARS-CoV-2 susceptibilityNon-fermentable (SWI/SNF) chromatinCoronavirus 2 infectionEnzyme 2 (ACE2) expressionSARS-CoV-2 variantsHuman cell typesPrimary human cell typesAirway epithelial cellsDrug-resistant variantsNew drug targetsChromatin accessibilitySNF complexACE2 locusACE2 expressionFactor complexHost determinantsTherapeutic targetConfer resistance
2019
Immune regulation by glucocorticoids can be linked to cell type–dependent transcriptional responses
Franco L, Gadkari M, Howe K, Sun J, Kardava L, Kumar P, Kumari S, Hu Z, Fraser I, Moir S, Tsang J, Germain R. Immune regulation by glucocorticoids can be linked to cell type–dependent transcriptional responses. Journal Of Experimental Medicine 2019, 216: 384-406. PMID: 30674564, PMCID: PMC6363437, DOI: 10.1084/jem.20180595.Peer-Reviewed Original ResearchConceptsCell typesHuman cell typesPrimary human cell typesCell-specific effectsTranscriptional outputTranscriptional regulationTranscriptional responseIndividual genesB cell receptorNew mechanistic understandingTranscriptional effectsImmunomodulatory cytokine IL-10Toll-like receptor 7Cytokine IL-10Immunoglobulin lociAnti-inflammatory drugsFunctional studiesMechanistic understandingBlimp-1Immunoregulatory therapyIL-10GenesCell receptorReceptor 7Glucocorticoid action
2010
Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA
Warren L, Manos PD, Ahfeldt T, Loh YH, Li H, Lau F, Ebina W, Mandal PK, Smith ZD, Meissner A, Daley GQ, Brack AS, Collins JJ, Cowan C, Schlaeger TM, Rossi DJ. Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell 2010, 7: 618-630. PMID: 20888316, PMCID: PMC3656821, DOI: 10.1016/j.stem.2010.08.012.Peer-Reviewed Original ResearchConceptsInduced pluripotent stem cellsPluripotent stem cellsCell fateMultiple human cell typesSomatic cell reprogrammingCell typesUseful cell typesStem cellsHuman cell typesPatient-specific induced pluripotent stem cellsCell reprogrammingCellular reprogrammingInnate antiviral responseDirected DifferentiationIPSC derivationHuman cellsMyogenic cellsSynthetic mRNAAntiviral responseDisease modelingReprogrammingModified mRNARegenerative medicineFateMRNAGenomics of Long-Range Regulatory Elements
Noonan JP, McCallion AS. Genomics of Long-Range Regulatory Elements. Annual Review Of Genomics And Human Genetics 2010, 11: 1-23. PMID: 20438361, DOI: 10.1146/annurev-genom-082509-141651.Peer-Reviewed Original ResearchConceptsRegulatory elementsGene expressionLong-range regulatory elementsCis-acting elementsHuman cell typesVertebrate genomesModel organismsSequence conservationTranscriptional regulationHuman genomeGenomic distanceRegulatory sequencesTransgenic technologyRegulatory potentialBiological functionsRegulatory functionsGenomeBiological significanceCell typesLarge-scale effortsRegulatory controlExpressionCommon setGenomicsGenesDirected differentiation of hematopoietic precursors and functional osteoclasts from human ES and iPS cells
Grigoriadis AE, Kennedy M, Bozec A, Brunton F, Stenbeck G, Park IH, Wagner EF, Keller GM. Directed differentiation of hematopoietic precursors and functional osteoclasts from human ES and iPS cells. Blood 2010, 115: 2769-2776. PMID: 20065292, PMCID: PMC2854424, DOI: 10.1182/blood-2009-07-234690.Peer-Reviewed Original ResearchConceptsPluripotent stem cellsInduced pluripotent stem cellsStem cellsPrimitive streak-like populationHuman pluripotent stem cellsHuman cell typesEmbryonic bone developmentEmbryoid bodiesBone-resorbing osteoclastsMacrophage colony-stimulating factorIPS cellsHematopoietic cytokinesCell typesMolecular analysisCathepsin KHuman ESHematopoietic precursorsPrecursor populationAlphavbeta3 integrinBone developmentConfocal microscopyAbsence of RANKLNuclear factor-kappaB ligandDisease mechanismsSerum-free medium
2008
Red cell membrane: past, present, and future
Mohandas N, Gallagher PG. Red cell membrane: past, present, and future. Blood 2008, 112: 3939-3948. PMID: 18988878, PMCID: PMC2582001, DOI: 10.1182/blood-2008-07-161166.Peer-Reviewed Original ResearchConceptsRed cell membraneCell membraneCell typesDiverse cell typesHuman cell typesOnly structural componentNatural selectionTransmembrane proteinSkeletal proteinsFunctional characterizationPlasma membraneMembrane envelopeNon-nucleated red cellsMembrane functionRed cell abnormalitiesProteinMembraneDisease statesCellsStructural componentsRed cellsNew lightUnexpected revelationExciting fieldCell abnormalities
1998
HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells
Uchida N, Sutton R, Friera A, He D, Reitsma M, Chang W, Veres G, Scollay R, Weissman I. HIV, but not murine leukemia virus, vectors mediate high efficiency gene transfer into freshly isolated G0/G1 human hematopoietic stem cells. Proceedings Of The National Academy Of Sciences Of The United States Of America 1998, 95: 11939-11944. PMID: 9751769, PMCID: PMC21744, DOI: 10.1073/pnas.95.20.11939.Peer-Reviewed Original ResearchMeSH KeywordsAntigens, CD34Base SequenceColony-Forming Units AssayDNA PrimersG1 PhaseGene ExpressionGene Transfer TechniquesGenes, ReporterGenetic TherapyGenetic VectorsGreen Fluorescent ProteinsHematopoietic Stem CellsHIV-1HumansIn Vitro TechniquesLeukemia Virus, MurineLuminescent ProteinsPhenotypeResting Phase, Cell CycleThy-1 AntigensTransduction, GeneticConceptsTransgene expressionHigh-efficiency gene transferSingle-step transductionGene delivery systemsGreen fluorescent proteinHematopoietic stem cellsHuman HSC subsetsVector supernatantsEfficient transductionTransduction efficiencyStem cellsHuman hematopoietic stem cellsMurine leukemia virusHIV vectorsDelivery systemGene transferGFP expressionHuman cell typesStem cell phenotypeReplication-defective HIVBone marrow stromal cellsLeukemia virusFluorescent proteinMarrow stromal cellsG0/G1
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