2024
Tetrameric PilZ protein stabilizes stator ring in complex flagellar motor and is required for motility in Campylobacter jejuni
Chen Y, Tachiyama S, Li Y, Feng X, Zhao H, Wu Y, Guo Y, Lara-Tejero M, Hua C, Liu J, Gao B. Tetrameric PilZ protein stabilizes stator ring in complex flagellar motor and is required for motility in Campylobacter jejuni. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 122: e2412594121. PMID: 39793078, PMCID: PMC11725899, DOI: 10.1073/pnas.2412594121.Peer-Reviewed Original ResearchConceptsFlagellar motorPilZ domain-containing proteinsBound cyclic di-GMPCyclic di-GMPC-di-GMPDomain-containing proteinsStator unitsDi-GMPFamily proteinsSuperfamily proteinsBacterial flagellaRing assemblyCellular pathwaysCampylobacter jejuniCryoelectron tomographyCampylobacter jejuni</i>.Subtomogram averagingPilZProteinFlagellaPhylumAncestorMotilityJejuniStructural componentsOverlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles
Park D, Fujise K, Wu Y, Luján R, Del Olmo-Cabrera S, Wesseling J, De Camilli P. Overlapping role of synaptophysin and synaptogyrin family proteins in determining the small size of synaptic vesicles. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2409605121. PMID: 38985768, PMCID: PMC11260120, DOI: 10.1073/pnas.2409605121.Peer-Reviewed Original ResearchConceptsSynaptic vesiclesFamily proteinsBiogenesis of synaptic vesiclesClusters of small vesiclesSize of synaptic vesiclesSynaptogyrin familySynaptogyrin-1Vesicle proteinsSynaptogyrinTransmembrane domainOrganismal levelSmall vesiclesProteinMild defectsVesiclesFamily membersBiogenesisSmall sizeFamilyMiceSynapsinCoexpressionAbundanceSynaptoporinMembersDistinct functional constraints driving conservation of the cofilin N-terminal regulatory tail
Sexton J, Potchernikov T, Bibeau J, Casanova-Sepúlveda G, Cao W, Lou H, Boggon T, De La Cruz E, Turk B. Distinct functional constraints driving conservation of the cofilin N-terminal regulatory tail. Nature Communications 2024, 15: 1426. PMID: 38365893, PMCID: PMC10873347, DOI: 10.1038/s41467-024-45878-9.Peer-Reviewed Original ResearchConceptsN-terminal regionActin bindingSequence requirementsLIM kinaseAnalysis of individual variantsInactivates cofilinS. cerevisiaeRegulatory tailFamily proteinsActin depolymerizationPhosphorylation sitesKinase recognitionSequence variantsInhibitory phosphorylationCofilinN-terminusIndividual variantsFunctional constraintsActinDisordered sequencesPhosphorylationSequenceBiochemical analysisSequence constraintsKinase
2023
Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure
Barger S, Penfield L, Bahmanyar S. Nuclear envelope assembly relies on CHMP-7 in the absence of BAF–LEM-mediated hole closure. Journal Of Cell Science 2023, 136: jcs261385. PMID: 37795681, PMCID: PMC10668030, DOI: 10.1242/jcs.261385.Peer-Reviewed Original ResearchConceptsNuclear envelope assemblySpindle microtubulesNE assemblyEnvelope assemblyC. elegans oocytesLEM-2C. elegansHelix domainBAF-1Family proteinsNucleoplasmic poolNE formationDistinct rolesMicrotubulesAdditional roleNE stabilityPermeability barrierRedundant mechanismsBAFProteinEmbryo survivalBindingAssemblyElegansAutointegrationKDM5 Lysine Demethylases in Pathogenesis, from Basic Science Discovery to the Clinic
Zhang S, Cao J, Yan Q. KDM5 Lysine Demethylases in Pathogenesis, from Basic Science Discovery to the Clinic. Advances In Experimental Medicine And Biology 2023, 1433: 113-137. PMID: 37751138, DOI: 10.1007/978-3-031-38176-8_6.ChaptersConceptsPlant homeodomainFamily proteinsKey epigenetic markCell fate determinationHistone methylation marksCancer type-dependent mannerKetoglutarate-dependent dioxygenasesSelective KDM5 inhibitorsTumor suppressive functionType-dependent mannerEpigenetic marksTumor suppressive roleFate determinationJumonji CLysine 4Active chromatinMethylation marksHistone H3Lysine demethylasesCatalytic coreKDM5 inhibitorsDrug targetsKDM5Cancer metastasisSuppressive roleInjury prevents Ras mutant cell expansion in mosaic skin
Gallini S, Annusver K, Rahman N, Gonzalez D, Yun S, Matte-Martone C, Xin T, Lathrop E, Suozzi K, Kasper M, Greco V. Injury prevents Ras mutant cell expansion in mosaic skin. Nature 2023, 619: 167-175. PMID: 37344586, PMCID: PMC10322723, DOI: 10.1038/s41586-023-06198-y.Peer-Reviewed Original ResearchConceptsWild-type cellsRas family proteinsCell cycle inhibitor p21Family proteinsOncogenic RasGenetic approachesMosaic tissuesInhibition of EGFRInhibitor p21EGFR ligandsEGFR pathwayCell expansionAberrant growthConstitutive lossDifferential activationParacrine secretionAbsence of injuryCellsCompetitive balanceInjury repairHealthy skinInjurySkinProteinPathwayCorrection: Trio family proteins as regulators of cell migration and morphogenesis in development and disease – mechanisms and cellular contexts
Bircher J, Koleske A. Correction: Trio family proteins as regulators of cell migration and morphogenesis in development and disease – mechanisms and cellular contexts. Journal Of Cell Science 2023, 136 PMID: 36763488, PMCID: PMC11034500, DOI: 10.1242/jcs.260984.Peer-Reviewed Original Research
2022
Novel pathways of intracellular membrane lipid transport and neurodegenerative diseases
De Camilli P. Novel pathways of intracellular membrane lipid transport and neurodegenerative diseases. The FASEB Journal 2022, 36 DOI: 10.1096/fasebj.2022.36.s1.0i152.ChaptersFamily proteinsLipid transportMembrane lipid transportMembrane contact sitesLipid binding modulesLipid transport proteinsLipid trafficRod-like proteinsBinding modulesHydrophobic grooveTransport proteinsContact sitesMembrane lipidsFunction mutationsLipid transferNovel pathwayNeurodevelopmental diseasesProteinVesicular carriersFamily resultsNeurodegenerative diseasesChorea-AcanthocytosisPotential roleNew mechanismMembraneChromatin profiling in human neurons reveals aberrant roles for histone acetylation and BET family proteins in schizophrenia
Farrelly L, Zheng S, Schrode N, Topol A, Bhanu N, Bastle R, Ramakrishnan A, Chan J, Cetin B, Flaherty E, Shen L, Gleason K, Tamminga C, Garcia B, Li H, Brennand K, Maze I. Chromatin profiling in human neurons reveals aberrant roles for histone acetylation and BET family proteins in schizophrenia. Nature Communications 2022, 13: 2195. PMID: 35459277, PMCID: PMC9033776, DOI: 10.1038/s41467-022-29922-0.Peer-Reviewed Original ResearchConceptsHistone posttranslational modificationsPosttranslational modificationsUnbiased proteomic approachPluripotent stem cellsPatient-derived neuronsH2A.Z acetylationChromatin profilingHyperacetylated histonesFamily proteinsProteomic approachProtein interactionsHistone acetylationTranscriptional abnormalitiesEpigenetic factorsExtraterminal (BET) proteinsSZ casesRisk variantsHuman neuronsStem cellsAberrant roleProtein inhibitionBona fideTreatment of schizophreniaPostmortem human brainCritical role
2021
Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1
Alim S, Cheppali S, Laitaoja M, Talluri T, Jänis J, Swamy M. Purification, molecular characterization and ligand binding properties of the major donkey seminal plasma protein DSP-1. International Journal Of Biological Macromolecules 2021, 194: 213-222. PMID: 34863837, DOI: 10.1016/j.ijbiomac.2021.11.177.Peer-Reviewed Original ResearchConceptsSperm plasma membraneSeminal plasma proteinsFibronectin type IIAmino acid sequenceIntrinsic fluorescence titrationBind to phosphorylcholineHSP-1/2PDC-109Family proteinsAcid sequenceCholine phospholipidsLigand binding propertiesEquus hemionusMultiple acetylationsSeminal plasmaPlasma membraneMolecular characterizationMammalian speciesMembrane perturbationProteinHigh-resolution LC-MS analysisProtein 1Head group moietyBinding propertiesCell membraneTrio family proteins as regulators of cell migration and morphogenesis in development and disease – mechanisms and cellular contexts
Bircher JE, Koleske AJ. Trio family proteins as regulators of cell migration and morphogenesis in development and disease – mechanisms and cellular contexts. Journal Of Cell Science 2021, 134: jcs248393. PMID: 33568469, PMCID: PMC7888718, DOI: 10.1242/jcs.248393.Peer-Reviewed Original ResearchConceptsFamily proteinsCellular contextProtein-protein interaction domainsHuman diseasesProtein trafficking pathwaysLarge multidomain proteinCell surface receptorsTrio proteinsUNC-73Cell morphogenesisProtein traffickingTrafficking pathwaysMultidomain proteinsInteraction domainInteraction partnersKey regulatorBiological contextTissue organizationCell migrationSurface receptorsProteinTrio familiesRecent discoveryMorphogenesisRegulator
2020
Role of VPS13, a protein with similarity to ATG2, in physiology and disease
Ugur B, Hancock-Cerutti W, Leonzino M, De Camilli P. Role of VPS13, a protein with similarity to ATG2, in physiology and disease. Current Opinion In Genetics & Development 2020, 65: 61-68. PMID: 32563856, PMCID: PMC7746581, DOI: 10.1016/j.gde.2020.05.027.Peer-Reviewed Original ResearchConceptsAutophagy protein ATG2N-terminal halfVPS13 proteinsMolecular functionsCellular processesFamily proteinsVps13Contact sitesAtg2Intracellular organellesFunctional studiesNovel mechanismProteinSimilar roleHydrophobic channelStructural studiesNeurodegenerative disordersPhysiologyDirect transferOrganellesSimilarityMutationsRoleLipidsBilayersSingle-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens
Vigneron A, O'Neill MB, Weiss BL, Savage AF, Campbell OC, Kamhawi S, Valenzuela JG, Aksoy S. Single-cell RNA sequencing of Trypanosoma brucei from tsetse salivary glands unveils metacyclogenesis and identifies potential transmission blocking antigens. Proceedings Of The National Academy Of Sciences Of The United States Of America 2020, 117: 2613-2621. PMID: 31964820, PMCID: PMC7007551, DOI: 10.1073/pnas.1914423117.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingRNA sequencingInfectious metacyclic formsMetacyclic parasitesMammalian host environmentFly salivary glandsMajor cell clustersClustering of cellsTsetse salivary glandsFamily proteinsDevelopmental programMammalian hostsMetacyclic cellsProtein transcriptsTrypanosoma bruceiDevelopmental processesGene expressionAfrican trypanosomesExpression profilesMolecular mechanismsSalivary glandsNew hostSurface localizationTrypanosome transmissionMetacyclogenesis
2019
Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1
Kumar A, Singh A, Bobde R, Vasudevan D. Structural Characterization of Arabidopsis thaliana NAP1-Related Protein 2 (AtNRP2) and Comparison with Its Homolog AtNRP1. Molecules 2019, 24: 2258. PMID: 31213016, PMCID: PMC6630525, DOI: 10.3390/molecules24122258.Peer-Reviewed Original ResearchConceptsNucleosome assembly proteinHistone chaperonesFamily proteinsNAP familyIsothermal titration calorimetry experimentsTitration calorimetry experimentsAssembly proteinOligomerization statusPlant tissuesSequence identityDimerization helixSimilar foldSimilar proteinsBiophysical studiesSpecific functionsStructural superpositionProtein 2ProteinPlantsChaperonesElectrophoretic mobilityStructural studiesCrystal structureThermal melting experimentsStructural architecture
2017
A Presynaptic Function of Shank Protein in Drosophila
Wu S, Gan G, Zhang Z, Sun J, Wang Q, Gao Z, Li M, Jin S, Huang J, Thomas U, Jiang YH, Li Y, Tian R, Zhang YQ. A Presynaptic Function of Shank Protein in Drosophila. Journal Of Neuroscience 2017, 37: 11592-11604. PMID: 29074576, PMCID: PMC6705749, DOI: 10.1523/jneurosci.0893-17.2017.Peer-Reviewed Original ResearchConceptsSynapse developmentMushroom bodiesPresynaptic functionHuman genetic studiesPostsynaptic densityPSD scaffold proteinsPeripheral neuromuscular junctionsNull mutantsMushroom body calyxScaffold proteinFamily genesFamily proteinsExpression analysisDevelopmental defectsShank proteinsGenetic studiesShank familyCalyx structureFunction mutationsOnly memberIdiopathic autism spectrum disorderSynaptic roleNovel insightsProteinDrosophilaExamination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations
Jonas E, Mnatsakanyan N. Examination of Mitochondrial Ion Conductance by Patch Clamp in Intact Neurons and Mitochondrial Membrane Preparations. Neuromethods 2017, 123: 211-238. DOI: 10.1007/978-1-4939-6890-9_11.Peer-Reviewed Original ResearchMitochondrial calcium uniporterMitochondrial permeability transition poreInner membraneCell deathOuter membraneIon channelsBcl-2 family proteinsNumerous cellular processesMitochondrial ion channelsComplex of proteinsChannel activityTrafficking of metabolitesPro-death stimuliMitochondrial membrane preparationsPermeability transition poreIon channel activityMembrane compartmentalizationIon channel complexDeath channelATP synthaseCellular processesFamily proteinsCalcium uniporterMolecular participantsATP productionNon-apoptotic functions of BCL-2 family proteins
Gross A, Katz SG. Non-apoptotic functions of BCL-2 family proteins. Cell Death & Differentiation 2017, 24: 1348-1358. PMID: 28234359, PMCID: PMC5520452, DOI: 10.1038/cdd.2017.22.Peer-Reviewed Original ResearchConceptsNon-apoptotic rolesBcl-2 family proteinsFamily proteinsApoptotic roleNon-apoptotic functionsWhole-cell metabolismCellular survival pathwaysMitochondrial physiologyCellular survivalSurvival pathwaysMajor regulatorNuclear processesApoptosis processProteinMechanism of actionPhysiologyImportant cluesRoleAutophagyRegulatorFascinating fieldRegulationPathwayMechanismMetabolismDistinct roles for TET family proteins in regulating human erythropoiesis
Yan H, Wang Y, Qu X, Li J, Hale J, Huang Y, An C, Papoin J, Guo X, Chen L, Kang Q, Li W, Schulz VP, Gallagher PG, Hillyer CD, Mohandas N, An X. Distinct roles for TET family proteins in regulating human erythropoiesis. Blood 2017, 129: 2002-2012. PMID: 28167661, PMCID: PMC5383871, DOI: 10.1182/blood-2016-08-736587.Peer-Reviewed Original ResearchConceptsMyelodysplastic syndromeErythroid differentiationHuman erythropoiesisErythroid progenitorsHuman erythroid differentiationTET family proteinsDistinct rolesKnockdown of TET2Terminal erythroid differentiationHuman erythroid cellsTET2 gene mutationsFamily proteinsTranslocation (TET) familyTET2 knockdownKnockdown experimentsErythroid cellsBiological processesDevelopment defectsTET3TET3 expressionOrthochromatic erythroblastsImpaired differentiationHuman CD34KnockdownTET2
2016
Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes
Bargieri D, Thiberge S, Tay C, Carey A, Rantz A, Hischen F, Lorthiois A, Straschil U, Singh P, Singh S, Triglia T, Tsuboi T, Cowman A, Chitnis C, Alano P, Baum J, Pradel G, Lavazec C, Ménard R. Plasmodium Merozoite TRAP Family Protein Is Essential for Vacuole Membrane Disruption and Gamete Egress from Erythrocytes. Cell Host & Microbe 2016, 20: 618-630. PMID: 27832590, PMCID: PMC5104695, DOI: 10.1016/j.chom.2016.10.015.Peer-Reviewed Original ResearchConceptsFamily proteinsGamete egressParasite motilityCell invasionErythrocyte invasionMembrane disruptionParasitophorous vacuole membraneMalaria parasite PlasmodiumBlood stage formsApicomplexan parasitesVacuole membraneExtracellular environmentParasite PlasmodiumActomyosin motorParasite transmissionTRAP proteinProteinCell egressStage formsInvasionEgressFalciparum parasitesParasitesIntegral roleMotilityBiogenesis of the Gram-positive bacterial cell envelope
Siegel S, Liu J, Ton-That H. Biogenesis of the Gram-positive bacterial cell envelope. Current Opinion In Microbiology 2016, 34: 31-37. PMID: 27497053, PMCID: PMC5164837, DOI: 10.1016/j.mib.2016.07.015.Peer-Reviewed Original ResearchConceptsWall teichoic acidGram-positive cell envelopeCell envelopeLipoteichoic acidLytR-CpsA-Psr family proteinsPhylum of Gram-positive bacteriaSortase-assembled piliCell envelope biogenesisBacterial cell envelopeGram-negative counterpartsGram-positive bacterial cell envelopeGram-positive bacteriaEnvelope biogenesisFamily proteinsTeichoic acidGram-negative organismsSurface displaySurface proteinsFolding mechanismOxidized proteinsGram-positiveCapsular polysaccharideDivergent functionsPiliBiogenesis
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