2025
PMA1-containing extracellular vesicles of Candida albicans triggers immune responses and colitis progression
Xu Z, Qiao S, Wang Z, Peng C, Hou Y, Liu B, Cao G, Wang T. PMA1-containing extracellular vesicles of Candida albicans triggers immune responses and colitis progression. Gut Microbes 2025, 17: 2455508. PMID: 39886799, PMCID: PMC11792855, DOI: 10.1080/19490976.2025.2455508.Peer-Reviewed Original ResearchConceptsPMA1 expressionExtracellular vesiclesCell differentiationAdaptor protein CARD9Th17 cell differentiationCARD9 deficiencyActivity of GAPDHVirulence factorsDecreased enzyme activityPma1Immune responseProteomic analysisCARD9Decreased glycolysisIL-17A productionMucosal immune responsesInduce Th17 cell differentiationMesenteric lymph nodesEditing systemAggravated colitisIL-17ALymph nodesColitis progressionAberrant changesGAPDH
2024
Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties.
Shin J, Park J, Lim J, Jeong J, Dinesh R, Maher S, Kim J, Park S, Hong J, Wysolmerski J, Choi J, Bothwell A. Metastasis of colon cancer requires Dickkopf-2 to generate cancer cells with Paneth cell properties. ELife 2024, 13 PMID: 39535280, PMCID: PMC11560131, DOI: 10.7554/elife.97279.Peer-Reviewed Original ResearchConceptsCancer cellsDickkopf-2Analysis of transcriptomeGeneration of cancer cellsPositive cancer cellsStem cell niche factorsColon cancer cellsPaneth cell differentiationHepatocyte nuclear factor 4 alphaLysozyme positive cellsChromatin accessibilityHNF4A proteinSingle-cell RNA sequencing analysisCell propertiesPaneth cell markersSequence analysisChromatin immunoprecipitationPromoter regionTranscription factorsTranscriptome analysisColon cancerColon cancer metastasisReduction of liver metastasisDownstream targetsCell differentiationNeuropeptide signalling orchestrates T cell differentiation
Hou Y, Sun L, LaFleur M, Huang L, Lambden C, Thakore P, Geiger-Schuller K, Kimura K, Yan L, Zang Y, Tang R, Shi J, Barilla R, Deng L, Subramanian A, Wallrapp A, Choi H, Kye Y, Ashenberg O, Schiebinger G, Doench J, Chiu I, Regev A, Sharpe A, Kuchroo V. Neuropeptide signalling orchestrates T cell differentiation. Nature 2024, 635: 444-452. PMID: 39415015, DOI: 10.1038/s41586-024-08049-w.Peer-Reviewed Original ResearchMeSH KeywordsActivating Transcription Factor 3AnimalsCalcitonin Gene-Related PeptideCalcitonin Receptor-Like ProteinCell DifferentiationCyclic AMP Response Element-Binding ProteinFemaleMaleMiceMice, Inbred C57BLReceptor Activity-Modifying Protein 3Signal TransductionSTAT1 Transcription FactorTh1 CellsTh2 CellsConceptsT helper type 1Acute viral infectionActivating transcription factor 3Th1 cell differentiationCAMP response element-binding proteinViral infectionCell differentiationNeuropeptide CGRPFate determinationT cellsCD8+ T cell responsesDifferentiation of Th2 cellsIn vitro polarizationT cell fate determinationT cell responsesTh1 cell responsesCell fate determinationIn vivo CRISPR screeningDownstream cAMP response element-binding proteinT cell differentiationT helper cell differentiationIn vivo differentiationResponse element-binding proteinElement-binding proteinNeuroimmune circuits6421 Imaging Phenotype May Predict Genotype In Human Pheochromocytoma: Relationships Between Genetic Mutation Clusters, Metabolite Transporter Expression, And Radiotracer Uptake
Sakuma I, Fujimoto M, Vatner D, Yokote K, Tanaka T. 6421 Imaging Phenotype May Predict Genotype In Human Pheochromocytoma: Relationships Between Genetic Mutation Clusters, Metabolite Transporter Expression, And Radiotracer Uptake. Journal Of The Endocrine Society 2024, 8: bvae163.210. PMCID: PMC11455308, DOI: 10.1210/jendso/bvae163.210.Peer-Reviewed Original ResearchGATA binding protein 3Expression of norepinephrine transporterGlucose transporter 1Chromaffin cell differentiationNorepinephrine transporter expressionNorepinephrine transporterFDG-PETGlucocorticoid receptorCluster 1 mutationsRadiotracer uptakeLevels of norepinephrine transportersGlucose transporter 1 expressionLocalization of pheochromocytomaChromaffin cell phenotypeCluster 2 tumorsExpression of glucose transporter 1Binding protein-3Expression levelsTumor gene expressionNorepinephrine transporter geneLevels of glucose transporter 1Cell differentiationTranscription factor Phox2aPositron emission tomographyFDG avidityIncreased autoreactivity and maturity of EBI2+ antibody-secreting cells from nasal polyps
Bai J, Kato A, Hulse K, Wechsler J, Gujar V, Poposki J, Harmon R, Iwasaki N, Wang B, Huang J, Stevens W, Conley D, Welch K, Kern R, Peters A, Eisenbarth S, Schleimer R, Tan B. Increased autoreactivity and maturity of EBI2+ antibody-secreting cells from nasal polyps. JCI Insight 2024, 9: e177729. PMID: 39253973, PMCID: PMC11385095, DOI: 10.1172/jci.insight.177729.Peer-Reviewed Original ResearchConceptsAntibody-secreting cellsGene Ontology biological processesSingle-cell RNA-seq analysisApoptosis pathwayNumbers of antibody-secreting cellsAnti-dsDNA IgGPlasma cell differentiationBiological processesCell differentiationNasal polypsGenesMolecular characteristicsNF-kBProliferative cellsMolecular featuresCellsAnti-double-stranded DNAIgG antibody-secreting cellsEBI2B cellsXBP1Recurrent NPElevated numbersDNAIncreased autoreactivityFunctional Roles of H3K4 Methylation in Transcriptional Regulation
Yu H, Lesch B. Functional Roles of H3K4 Methylation in Transcriptional Regulation. Molecular And Cellular Biology 2024, 44: 505-515. PMID: 39155435, PMCID: PMC11529435, DOI: 10.1080/10985549.2024.2388254.Peer-Reviewed Original ResearchTranscriptional regulationAssociated with active transcriptionHistone 3 lysine 4 methylationFunctional roleTranscribed lociOpen chromatinActivate transcriptionChromatin modificationsH3K4 methylationRegulatory elementsHistone methyltransferaseEpigenetic editingTranscriptional activityResidue mutationsMammalian systemsCell differentiationHistoneH3K4me1H3K4meH3K4me3ChromatinRegulationH3K4YeastLociA humanized mouse that mounts mature class-switched, hypermutated and neutralizing antibody responses
Chupp D, Rivera C, Zhou Y, Xu Y, Ramsey P, Xu Z, Zan H, Casali P. A humanized mouse that mounts mature class-switched, hypermutated and neutralizing antibody responses. Nature Immunology 2024, 25: 1489-1506. PMID: 38918608, PMCID: PMC11291283, DOI: 10.1038/s41590-024-01880-3.Peer-Reviewed Original ResearchConceptsB cellsImmune cell differentiationAntibody responseHumanized micePfizer-BioNTech coronavirus disease 2019T cell antigen receptor repertoireFollicular helper T cellsGerminal center B cellsHuman cord blood CD34Human thymic epithelial cellsT cell-independent antibody responsesImmune systemMarginal zone B cellsMaturation of antibody responsesMemory B cell differentiationCD34+ cellsCord blood CD34Helper T cellsT cell-dependentThymic epithelial cellsNeutralizing antibody responsesB cell differentiationHuman B cellsAntigen receptor repertoireCell differentiationGut microbiota from B-cell-specific TLR9-deficient NOD mice promote IL-10+ Breg cells and protect against T1D
Yang X, Huang J, Peng J, Wang P, Wong F, Wang R, Wang D, Wen L. Gut microbiota from B-cell-specific TLR9-deficient NOD mice promote IL-10+ Breg cells and protect against T1D. Frontiers In Immunology 2024, 15: 1413177. PMID: 38903498, PMCID: PMC11187306, DOI: 10.3389/fimmu.2024.1413177.Peer-Reviewed Original ResearchGut microbiotaGerm-free miceToll-like receptor 9Increased gut permeabilityIntestinal microbiotaGut permeabilityT1D developmentGut microbiota compositionFecal samplesTransferred to germ-free miceGut barrier integrityBreg cell differentiationMicrobiota influenceMucin degradationMicrobiota compositionBreg cellsAltered microbiotaMicrobiota impactMicrobiotaGene expressionImmune regulationDevelopment of T1DCell differentiationGutNOD miceCentral neurocytoma exhibits radial glial cell signatures with FGFR3 hypomethylation and overexpression
Lee Y, Chowdhury T, Kim S, Yu H, Kim K, Kang H, Kim M, Kim J, Kim Y, Ji S, Hwang K, Han J, Hwang J, Yoo S, Lee K, Choe G, Won J, Park S, Lee Y, Shin J, Park C, Kim C, Kim J. Central neurocytoma exhibits radial glial cell signatures with FGFR3 hypomethylation and overexpression. Experimental & Molecular Medicine 2024, 56: 975-986. PMID: 38609519, PMCID: PMC11059271, DOI: 10.1038/s12276-024-01204-3.Peer-Reviewed Original ResearchConceptsWhole-exome sequencingNeuronal development pathwaysDrivers of tumorigenesisGlial cell differentiationMethylation sequencingGenomic eventsPI3K-Akt activationDownstream eventsGene markersMultiomics approachCell differentiationRadial glial cellsHypomethylationOverexpressionSequenceTumorigenesisFGFR3Cell signaturesGlial cellsPotential roleCellsTumor cellsCentral nervous systemMultiomicsOntogeny
2023
Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasis
Reyes E, Castillo-Azofeifa D, Rispal J, Wald T, Zwick R, Palikuqi B, Mujukian A, Rabizadeh S, Gupta A, Gardner J, Boffelli D, Gartner Z, Klein O. Epithelial TNF controls cell differentiation and CFTR activity to maintain intestinal mucin homeostasis. Journal Of Clinical Investigation 2023, 133: e163591. PMID: 37643009, PMCID: PMC10575728, DOI: 10.1172/jci163591.Peer-Reviewed Original ResearchConceptsCystic fibrosis transmembrane conductance regulatorTumor necrosis factorInflammatory bowel diseaseCell differentiationRegulate mucin productionCystic fibrosis transmembrane conductance regulator inhibitionCystic fibrosis transmembrane conductance regulator activityTumor necrosis factor treatmentInflammatory cytokine tumor necrosis factorAnti-TNF therapyAbsence of tumor necrosis factorTransmembrane conductance regulatorCytokine tumor necrosis factorMucus-producing goblet cellsGenetic mouse modelsUpstream regulatorIncreased mucus accumulationAdult intestineConductance regulatorEpithelial signalsSignaling axisProgenitor cellsMouse modelNecrosis factorGut transit timeMammalian SWI/SNF chromatin remodeling complexes promote tyrosine kinase inhibitor resistance in EGFR-mutant lung cancer
de Miguel F, Gentile C, Feng W, Silva S, Sankar A, Exposito F, Cai W, Melnick M, Robles-Oteiza C, Hinkley M, Tsai J, Hartley A, Wei J, Wurtz A, Li F, Toki M, Rimm D, Homer R, Wilen C, Xiao A, Qi J, Yan Q, Nguyen D, Jänne P, Kadoch C, Politi K. Mammalian SWI/SNF chromatin remodeling complexes promote tyrosine kinase inhibitor resistance in EGFR-mutant lung cancer. Cancer Cell 2023, 41: 1516-1534.e9. PMID: 37541244, PMCID: PMC10957226, DOI: 10.1016/j.ccell.2023.07.005.Peer-Reviewed Original ResearchConceptsMammalian SWI/SNF chromatinSWI/SNF chromatinMSWI/SNF complexesGenome-wide localizationGene regulatory signaturesNon-genetic mechanismsEpithelial cell differentiationEGFR-mutant cellsChromatin accessibilitySNF complexCellular programsRegulatory signaturesTKI-resistant lung cancerGene targetsKinase inhibitor resistanceCell differentiationMesenchymal transitionTKI resistancePharmacologic disruptionTyrosine kinase inhibitor resistanceCell proliferationChromatinInhibitor resistanceEGFR-mutant lungKinase inhibitorsRole of homeobox d10 gene targeted signaling pathways in cancers
Surendran H, Palaniyandi T, Natarajan S, Hari R, Viwanathan S, Baskar G, Abdul Wahab M, Ravi M, Rajendran B. Role of homeobox d10 gene targeted signaling pathways in cancers. Pathology - Research And Practice 2023, 248: 154643. PMID: 37406379, DOI: 10.1016/j.prp.2023.154643.Peer-Reviewed Original ResearchConceptsTranscription factorsHomeobox gene familyHomeotic transcription factorsGene expression changesTumor suppressor geneD10 geneGene familyHomeobox genesCancer signalingOrgan developmentTissue homeostasisGene expressionExpression changesSignaling pathwaysCell differentiationSuppressor geneFunctional interactionGenesHOXD10 geneCancer treatment targetHOXD10Pathway dysregulationTherapeutic resistancePathwayMolecule actionSOX9 Governs Gastric Mucous Neck Cell Identity and Is Required for Injury-Induced Metaplasia
Willet S, Thanintorn N, McNeill H, Huh S, Ornitz D, Huh W, Hoft S, DiPaolo R, Mills J. SOX9 Governs Gastric Mucous Neck Cell Identity and Is Required for Injury-Induced Metaplasia. Cellular And Molecular Gastroenterology And Hepatology 2023, 16: 325-339. PMID: 37270061, PMCID: PMC10444955, DOI: 10.1016/j.jcmgh.2023.05.009.Peer-Reviewed Original ResearchConceptsSRY-box transcription factor 9Cell identityAdult homeostasisGastric progenitorsMucous neck cellsZymogenic chief cellsGastric developmentNeck cellsPotential genesMaster regulatorExpression patternsGene expressionSPEM cellsCell differentiationCorpus unitsSOX9 expressionSOX9Factor 9Specific expansionHomeostasisMisexpressionSox9 deletionReprogrammingChief cellsGenetic deletionSerendipitous Discovery of T Cell–Produced KLK1b22 as a Regulator of Systemic Metabolism
Arwood M, Sun I, Patel C, Sun I, Oh M, Bettencourt I, Claiborne M, Chan-Li Y, Zhao L, Waickman A, Mavrothalassitis O, Wen J, Aja S, Powell J. Serendipitous Discovery of T Cell–Produced KLK1b22 as a Regulator of Systemic Metabolism. ImmunoHorizons 2023, 7: 493-507. PMID: 37358498, PMCID: PMC10580127, DOI: 10.4049/immunohorizons.2300016.Peer-Reviewed Original ResearchConceptsGlucose toleranceT cellsSystemic metabolismGenome ProjectWild-type T cellsMicroarray analysisCell differentiationNovel roleRhebMammalian targetInsulin receptorT cell differentiationReduced glucose toleranceMarked increaseStrains of miceBeige fatExpressionInsulin sensitivityOverexpressionSystemic overexpressionMetabolismCellsMiceToleranceFurther studiesCentralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding
Davidson K, Nakamura M, Verboon J, Parkhurst S. Centralspindlin proteins Pavarotti and Tumbleweed along with WASH regulate nuclear envelope budding. Journal Of Cell Biology 2023, 222: e202211074. PMID: 37163553, PMCID: PMC10174194, DOI: 10.1083/jcb.202211074.Peer-Reviewed Original ResearchConceptsNuclear envelope buddingActin nucleation activityNuclear export pathwayNE buddingExport pathwayWiskott-Aldrich syndrome proteinProtein quality controlActin-bundling activityNew molecular componentsCargo recruitmentSyndrome proteinRegulatory complexMitochondrial integritySynapse developmentCell differentiationStructural roleMolecular componentsNuclear washesLarge cargoBuddingPavarottiBudsMachineryTumbleweedNew entry pointAdrenergic receptors regulate T cell differentiation in viral infection and cancer
Globig A, Zhao S, Roginsky J, Avina-Ochoa N, Heeg M, Chaudhary O, Hoffmann F, Chen D, O’Connor C, Emu B, Kaech S. Adrenergic receptors regulate T cell differentiation in viral infection and cancer. The Journal Of Immunology 2023, 210: 59.13-59.13. DOI: 10.4049/jimmunol.210.supp.59.13.Peer-Reviewed Original ResearchImmune checkpoint blockadeT cell differentiationChronic viral infectionsT cell functionalityT cellsViral infectionAdrenergic receptorsChronic antigen exposureLCMV clone 13Novel immune checkpointT cell proliferationMurine cancer modelsT cell receptor signalingCell differentiationAbstract CD8Terminal-CD8Exhausted CD8Checkpoint blockadeAntigen exposureImmune checkpointsNoradrenaline levelsCell receptor signalingTumor sizeCancer patientsNA receptorsIntrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells
Rivera C, Zhou Y, Chupp D, Yan H, Fisher A, Simon R, Zan H, Xu Z, Casali P. Intrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells. The Journal Of Immunology 2023, 210: 60.08-60.08. DOI: 10.4049/jimmunol.210.supp.60.08.Peer-Reviewed Original ResearchClass switch DNA recombinationGeneration of memory B cellsAbsence of T cellsMemory B cellsT cell helpNeutralizing antibody responsesPlasma cell differentiationAntibody responseT cellsB cellsCell differentiationAnti-microbial responsesSomatic hypermutationT cell compartmentT cell functionAntibodies to E. coliDeclining T cell functionAffinity maturation processS. typhimuriumDNA recombinationMature antibody responseAnamnestic antibody responseLate-stage responsesSalmonella flagellinPhysical linkageRORα plays an important role in generation and maintenance of memory B cells
Xu Y, Moroney J, Zhou Y, Zan H, Casali P. RORα plays an important role in generation and maintenance of memory B cells. The Journal Of Immunology 2023, 210: 76.02-76.02. DOI: 10.4049/jimmunol.210.supp.76.02.Peer-Reviewed Original ResearchMemory B cellsMaintenance of memory B cellsB cellsNP-CGGAnti-NP antibody responseAnti-NP responsesClass-switched antibodiesAbstract Memory B cellsGerminal center formationActivated B cellsPlasma cell differentiationResponse to immunizationPlasma cellsAntigen-specificSecrete large amountsRNA-seq experimentsClass switch DNA recombinationClass switchingSomatic hypermutationMiceTranscriptional targetsCenter formationChromatin landscapeCell differentiationRNA-seqIntrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells
Rivera C, Zhou Y, Chupp D, Yan H, Fisher A, Simon R, Zan H, Xu Z, Casali P. Intrinsic B cell TLR-BCR linked coengagement induces class-switched, hypermutated, neutralizing antibody responses in absence of T cells. Science Advances 2023, 9: eade8928. PMID: 37115935, PMCID: PMC10146914, DOI: 10.1126/sciadv.ade8928.Peer-Reviewed Original ResearchConceptsClass switch DNA recombinationT cell helpPlasma cell differentiationAntibody responseCell differentiationSomatic hypermutationGeneration of memory B cellsInduce neutralizing antibody responsesAbsence of T cellsMaturation of antibody responsesMemory B cellsNeutralizing antibody responsesResponse to NPDNA recombinationAnamnestic antibody responseMicrobial pathogensFlagellinT cellsB cellsClonal expansionClass switchingProtective antibodiesCoengagementCell receptorsDifferentiationLive imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo
May D, Yun S, Gonzalez D, Park S, Chen Y, Lathrop E, Cai B, Xin T, Zhao H, Wang S, Gonzalez L, Cockburn K, Greco V. Live imaging reveals chromatin compaction transitions and dynamic transcriptional bursting during stem cell differentiation in vivo. ELife 2023, 12: e83444. PMID: 36880644, PMCID: PMC10027315, DOI: 10.7554/elife.83444.Peer-Reviewed Original ResearchConceptsStem cell differentiationCell differentiationStem cell compartmentCompaction changesChromatin compaction statesDynamic transcriptional statesCell compartmentChromatin architectureCell cycle statusChromatin rearrangementNascent RNATranscriptional burstingTranscriptional statesLive imagingTissue contextGene expressionDifferentiating cellsGlobal remodelingIndividual cellsCycle statusStem cellsDifferentiation statusDifferentiationCellsMorphological changes
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