2025
Genome-scale CRISPR/Cas9 screening reveals the role of PSMD4 in colibactin-mediated cell cycle arrest
Dougherty M, Hoffmann R, Hernandez M, Airan Y, Gharaibeh R, Herzon S, Yang Y, Jobin C. Genome-scale CRISPR/Cas9 screening reveals the role of PSMD4 in colibactin-mediated cell cycle arrest. MSphere 2025, 10: e00692-24. PMID: 39918307, PMCID: PMC11934320, DOI: 10.1128/msphere.00692-24.Peer-Reviewed Original ResearchConceptsCell cycle arrestCycle arrestCRISPR/Cas9 screenDNA damageGenome-scale CRISPR/Cas9 screensGenome-wide CRISPR/Cas9 knockout screenRegulating cell cycle arrestRNA polymerase IIIRNA processing factorsCRISPR/Cas9 knockout screenG2-M cell cycle arrestGenotoxic secondary metabolitesHT-29 cellsHost response pathwaysPolymerase IIIKnockout screenHost pathwaysCell fateProteasome subunitsColony sizeColibactin-producing bacteriaColibactinRNA sequencingColony formation rateGene expression
2023
DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner
Strine M, Cai W, Wei J, Alfajaro M, Filler R, Biering S, Sarnik S, Chow R, Patil A, Cervantes K, Collings C, DeWeirdt P, Hanna R, Schofield K, Hulme C, Konermann S, Doench J, Hsu P, Kadoch C, Yan Q, Wilen C. DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner. PLOS Biology 2023, 21: e3002097. PMID: 37310920, PMCID: PMC10263356, DOI: 10.1371/journal.pbio.3002097.Peer-Reviewed Original ResearchConceptsGenome-wide CRISPR/Cas9 screenCRISPR/Cas9 screenPathogenic human coronavirusesKinase-independent mannerRegulated kinase 1AProviral host factorNovel drug targetsMultiple cell typesDNA accessibilityHost factorsKinase functionHuman coronavirusesHost genesDistal enhancerNovel regulatorCas9 screenKinase 1AGene expressionNeuronal developmentDYRK1ADrug targetsDiverse coronavirusesProviral activityCell typesSevere acute respiratory syndrome coronavirus 2
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