Distinct polyfunctional CD4+ T cell responses to BCG, ESAT-6 and CFP-10 in tuberculous pleurisy
Li L, Qiao D, Li Q, Zhang X, Lao S, Wu C. Distinct polyfunctional CD4+ T cell responses to BCG, ESAT-6 and CFP-10 in tuberculous pleurisy. Tuberculosis 2011, 92: 63-71. PMID: 22154006, DOI: 10.1016/j.tube.2011.11.004.Peer-Reviewed Original ResearchConceptsPleural fluid cellsESAT-6T cellsMedian fluorescence intensityTuberculous pleurisyCFP-10IL-2Effector/effector memory phenotypeAntigen-specific T cellsFrequent extrapulmonary manifestationMtb-specific antigensEffector memory phenotypeT cell responsesCFP-10 peptidesExtrapulmonary manifestationsPolyfunctional CD4Th1 cytokinesTuberculosis infectionMemory phenotypeInflammatory cellsTh1 cellsSpecific antigenLocal controlCell responsesFollowing StimulationHuman natural killer cells expressing the memory‐associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO− natural killer cells following stimulation with interleukin‐12
Fu X, Liu Y, Li L, Li Q, Qiao D, Wang H, Lao S, Fan Y, Wu C. Human natural killer cells expressing the memory‐associated marker CD45RO from tuberculous pleurisy respond more strongly and rapidly than CD45RO− natural killer cells following stimulation with interleukin‐12. Immunology 2011, 134: 41-49. PMID: 21711347, PMCID: PMC3173693, DOI: 10.1111/j.1365-2567.2011.03464.x.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAntigens, CDAntigens, Differentiation, T-LymphocyteCD56 AntigenCell CountCytotoxicity, ImmunologicFemaleGPI-Linked ProteinsGranzymesHumansInterferon-gammaInterleukin-12Interleukin-2 Receptor alpha SubunitK562 CellsKiller Cells, NaturalLectins, C-TypeLeukocyte Common AntigensLeukocytes, MononuclearLymphocyte ActivationLymphocyte SubsetsMaleMiddle AgedNK Cell Lectin-Like Receptor Subfamily CNK Cell Lectin-Like Receptor Subfamily KPleural EffusionReceptors, IgGReceptors, Interleukin-12Tuberculosis, PleuralYoung AdultConceptsPleural fluid cellsNatural killer cellsNK cellsKiller cellsInterleukin-12Human natural killer cellsAdaptive immune featuresCD45RO- NK cellsInnate immune lymphocytesHuman NK cellsIL-12 receptor β1Immune lymphocytesIL-12Tuberculous patientsTuberculous pleurisyImmune featuresGranzyme BMore interferonReceptor β1Mycobacterium tuberculosisCD45ROFluid cellsPresent studyCellsCD25Pleural fluid from tuberculous pleurisy inhibits the functions of T cells and the differentiation of Th1 cells via immunosuppressive factors
Li Q, Li L, Liu Y, Fu X, Qiao D, Wang H, Lao S, Huang F, Wu C. Pleural fluid from tuberculous pleurisy inhibits the functions of T cells and the differentiation of Th1 cells via immunosuppressive factors. Cellular & Molecular Immunology 2011, 8: 172-180. PMID: 21258363, PMCID: PMC4003141, DOI: 10.1038/cmi.2010.80.Peer-Reviewed Original ResearchConceptsT cell functionT cellsIL-10Tuberculosis pleurisyTh1 cellsLocal immune statusCourse of diseaseTumor necrosis factorTh1 cell differentiationGrowth factor-β1Inhibitor of indoleamineImmunosuppressive mediatorsEffect of PFImmunosuppressive factorsCytokine productionImmune statusTuberculous pleurisyPleural fluidImmunopathological factorsFactor-β1Cell activationPleurisyGrowth factorCell cycle progressionCycle progression[Analysis of ESAT-6-specific multifunctional CD4+; T cells in pleural fluid from patients with tuberculous pleurisy].
Li L, Qiao D, Li Q, Zhang X, Lao S, Wu C. [Analysis of ESAT-6-specific multifunctional CD4+; T cells in pleural fluid from patients with tuberculous pleurisy]. Chinese Journal Of Cellular And Molecular Immunology 2011, 27: 26-8. PMID: 21208559.Peer-Reviewed Original ResearchConceptsRecombinant ESAT-6 proteinT cellsESAT-6 proteinCytokine productionMean fluorescence intensityPeptide poolsTB infectionIL-2Positive cellsTh1 cytokine productionSingle-positive cellsTriple-positive cellsTuberculous pleurisyESAT-6Cell levelSingle-cell levelFlow cytometrySimilar frequencyStimulationCytokinesIFNTNFInfectionBCGCells