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Visualizing Tissues and Gene Products

Sorting embryos with epifluorescent microscope. Photo by Robert Lisak.

In situ Hybridization - methods and easy checklists for performing in situ hybridization to detect mRNA

Ab Staining - protocols for immunohistochemistry

TUNEL Staining - protocol to detect apoptotic cells in whole mount

Cartilage Staining - uses alcian blue and alizarin red dyes to visualize the cartilage and bone

Sectioning Embryos - protocols for vibratome sections of embryos

H&E Staining of Sections - protocols for H&E staining as well as light counterstains

Topicalis in situ Library - pictures of some stains using various probes on the Harland Website.