Obtaining Embryos

hCG Injection - Injecting trops with hCG to induce ovulation

Natural Matings - How to setup matings between males and females to harvest eggs

Isolating Testis - for in vitro fertilization (IVF) of eggs

Squeezing Females - to get eggs for IVF

High Salt Protocol - prolong the viability of eggs before IVF

IVF - how to perform in vitro fertilization and maximize your fertilization rate

De-Jelly - protocol for removing the jelly coats of embryos

Sperm Freezing and Storage - X. tropicalis sperm can be frozen for long term storage of lines and then later thawed to recover the line.

Developmental Timetables - allows the experimentalist to track the developmental stage of embryos over time for particular ambient temperatures.

NOTE: Whenever handling X. tropicalis, we recommend wearing gloves (powder-free). X. tropicalis have been shown to harbor mycobacterial infections. While we have had no instances of laboratory transmission of mycobacterial disease from frogs to humans, we recommend wearing gloves until the mycobacterial infection is fully characterized. Currently, we believe the risk of human infection of mycobacteria from frogs is quite low and the symptoms would be mild (cutaneous ulcer that can be treated with excision). Nevertheless, avoiding infection remains the goal so we recommend gloves as protection. For the protection of the frogs, it is important that the gloves be powder-free.