Wnt Signaling Research

Wnt Signaling

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Wnt signaling is essential for axis formation. By overexpression of wnt8 or b-catenin, we can induce secondary axes in frogs and create tadpoles with two body axes.
Canonical Wnt signaling requires that b-catenin enter the nucleus in order to activate wnt target genes. By tagging b-catenin with GFP, we can monitor the localization of b-catenin at the cell cortex or in the nucleus.
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The image at left depicts nuclear localization of beta-catenin in stage 10 xenopus embryo. J. Griffin.