Imaging
In our approach, we analyze genes from patients with birth defects in order to understand how they affect development. Then, in order to understand how the gene affects embryonic development, we need to see developmental events in action. In particular, we need to be able to watch the behavior of cells or subcellular organelles during development. Live imaging can be challenging but incredibly informative. In addition, we can label different parts of the cell or monitor dynamic cellular components (actin, calcium, etc) with new fluorophores in order to understand the processes that shape an embryo. In addition, microscopes continue to become more sensitive with greater resolution, allowing the visualization of things we could never see before. In addition to confocal microscopy, we are interested in lightsheet and super-resolution imaging methods to look into the embryo and see embryonic development in action.
Nikon A1R confocal
Neurotubulin-GFP transtenic, phalloidin, DAPI
2ᵒ antibody: Alexa Fluor 488 (GFP) 1:500
Phalloidin 568 F-actin (RFP) 1:500
By Cindy Kha , Xenopus Course CSHL, on Nikon A1R confocal.
Cilia IFT
membrane-RFP and IFT80-GFP, Xenopus epidermal cilia.
Epidermal Cilia
Xenopus epidermal cilia labelled with CLAMP-GFP and memRFP at CSHL Xenopus Course with Quantitative Imaging.
Bruker swept field confocal.
OTC Imaging
Ciliary IFT in WT embryo
Protrusions-60_480px
mem-RFP, H2B-GFP
Imaged on Nikon A1R - Robert Huebner
Cold Spring Harbor Lab - Xenopus Course
Cell Division
Imaged on Nikon A1R - Robert Huebner
Cold Spring Harbor Lab - Xenopus Course