Request Frogs

We have a number of frogs available for distribution. For more information, please send your requests to Maura Lane (maura.lane@yale.edu).

Inbred Lines
Name	Available As	Description
Nigerian	Mating Pair	These are inbred Nigerian X. tropicalis kindly provided to the Harland Lab by Rob Grainger and bred in the lab. These frogs have further been inbred in the Khokha Lab (F20 as of 01/22).
Nigerian "Superman"	Males	These are inbred animals are derived from a Nigerian male who showed very early nuptial pads and had very good fertilization rates. We have inbred a line arising from "Superman", and selected for early nuptial pads/maturation/good fert rates.
Nigerian	Females	These females are also derived from Superman. They have been bred to lay large amounts of good quality eggs for microinjection and breeding purposes.
Ivory Coast B (ICB) "blue" or "Axanthic"	Males/Females	ICB strain frogs appear to be richly polymorphic compared to Nigerian. In addition, they have a pigmentation defect, making them easy to distinguish.

-Tim Grammer, Mustafa Khokha, Maura Lane

last updated by ML, 04/22

Pigment Mutants
Name	Available As	Description
EDN	Adult Female and Male	K/O of endodermin3. Adult animals are pigment-less, but have pigmented eyes.
Tyrosinase	Adult Female and Male	These frogs derive from tryosinase KO embryos created using CRISPR/Cas9.

-Tim Grammer, Mustafa Khokha, Maura Lane

last updated by ML, 04/22

Tissue Specific Mutants
Name	Available As	Description
T2XIG	Adult Female and Male	Ubiquitous GFP
xt_Tubb2b:GCamp6S	Adult Female and Male	Founders were obtained from NXR at MBL. Created by
Tubb:gCAMP6s/Tyrosinase	Adult Female and Male	Tubb:gCAMP6s line crossed with Tyrosinase K/O. Tubb:gCAMP6s. Founders were obtained from NXR at MBL. Created by
Triple	Adult Female and Male	Our founders provided by the Grainger lab. Triple transgenic (Grainger stock 399) Pax6GFP/Cardiac Actin GFP/gamma crystallin RFP. Transgenes can be seen at St. 45.
Triple/Tyrosinase	Adult Female and Male
pFlk	Adult Female and Male	This line has tissue specific Flk expression (GFP in heart and vasculature), created using Tol2
pFLK/Tyrosinse	Adult Female and Male	pFlk line crossed with Tyrosinase K/O
Tubb	Adult Female and Male
Tubb/Tyrosinase	Adult Female and Male	Tubb line crossed with Tyrosinase K/O
Otx2	Adult Female and Male
Otx2/Tyrosinase	Adult Female and Male	Otx2 line crossed with Tyrosinase K/O
pMLC1v	Adult Female and Male
pMLC1v/Tyrosinase	Adult Female and Male	pMLC1v line crossed with Tyrosinase K/O
Tubb/Tyrosinase	Adult Female and Male
Xtr.Tg(pbin7LEF-GFP)	Adult Female and Male	Wnt reporter, GFP in brain. Homozygous and herterozygous available.
Xtr.Tg(pbin7LEF-GFP)/Tyrosinase	Adult Female and Male	Tyrosinase K/O crossed with 1Lef line. Wnt reporter, GFP in brain. Homozygous and herterozygous available
Brachyury/Cre	Adult Female and Male	GFP eye marker
Cre-CFP	Adult Female and Male	Cre Reporter. GFP eyes and ubiquitous CFP

-Tim Grammer, Mustafa Khokha, Maura Lane

last updated by ML, 04/22