Skip to Main Content

INFORMATION FOR

Request Frogs

Inbred Strains

We have a number of frogs available for distribution. For more information, please send your requests to Maura Lane (maura.lane@yale.edu).

Inbred Lines
Name Available As Description
Nigerian Mating Pair These are inbred Nigerian X. tropicalis kindly provided to the Harland Lab by Rob Grainger and bred in the lab. These frogs have further been inbred in the Khokha Lab (F20 as of 01/22).

Nigerian "Superman" Males These are inbred animals are derived from a Nigerian male who showed very early nuptial pads and had very good fertilization rates. We have inbred a line arising from "Superman", and selected for early nuptial pads/maturation/good fert rates.
Nigerian Females These females are also derived from Superman. They have been bred to lay large amounts of good quality eggs for microinjection and breeding purposes.
Ivory Coast B (ICB) "blue" or "Axanthic" Males/Females ICB strain frogs appear to be richly polymorphic compared to Nigerian. In addition, they have a pigmentation defect, making them easy to distinguish.




-Tim Grammer, Mustafa Khokha, Maura Lane

last updated by ML, 04/22

Pigment Mutants

Pigment Mutants
Name Available As Description
EDN Adult Female and Male K/O of endodermin3. Adult animals are pigment-less, but have pigmented eyes.
Tyrosinase Adult Female and Male These frogs derive from tryosinase KO embryos created using CRISPR/Cas9.



-Tim Grammer, Mustafa Khokha, Maura Lane

last updated by ML, 04/22

Tissue Specific Transgenics

Tissue Specific Mutants
Name Available As Description
T2XIG Adult Female and Male Ubiquitous GFP
xt_Tubb2b:GCamp6S Adult Female and Male Founders were obtained from NXR at MBL. Created by
Tubb:gCAMP6s/Tyrosinase Adult Female and Male Tubb:gCAMP6s line crossed with Tyrosinase K/O. Tubb:gCAMP6s. Founders were obtained from NXR at MBL. Created by
Triple Adult Female and Male Our founders provided by the Grainger lab. Triple transgenic (Grainger stock 399) Pax6GFP/Cardiac Actin GFP/gamma crystallin RFP. Transgenes can be seen at St. 45.
Triple/Tyrosinase Adult Female and Male
pFlk Adult Female and Male This line has tissue specific Flk expression (GFP in heart and vasculature), created using Tol2
pFLK/Tyrosinse Adult Female and Male pFlk line crossed with Tyrosinase K/O
Tubb Adult Female and Male
Tubb/Tyrosinase Adult Female and Male Tubb line crossed with Tyrosinase K/O
Otx2 Adult Female and Male
Otx2/Tyrosinase Adult Female and Male Otx2 line crossed with Tyrosinase K/O
pMLC1v Adult Female and Male
pMLC1v/Tyrosinase Adult Female and Male pMLC1v line crossed with Tyrosinase K/O
Tubb/Tyrosinase Adult Female and Male
Xtr.Tg(pbin7LEF-GFP) Adult Female and Male Wnt reporter, GFP in brain. Homozygous and herterozygous available.
Xtr.Tg(pbin7LEF-GFP)/Tyrosinase Adult Female and Male Tyrosinase K/O crossed with 1Lef line. Wnt reporter, GFP in brain. Homozygous and herterozygous available
Brachyury/Cre Adult Female and Male GFP eye marker
Cre-CFP Adult Female and Male Cre Reporter. GFP eyes and ubiquitous CFP

-Tim Grammer, Mustafa Khokha, Maura Lane

last updated by ML, 04/22