2013
Increasing dietary protein selectively upregulates the DMT1 isoform that contains an iron responsive element
Thomas C, Yao G, O'Brien K, Kerstetter J, Insogna K. Increasing dietary protein selectively upregulates the DMT1 isoform that contains an iron responsive element. The FASEB Journal 2013, 27: lb284-lb284. DOI: 10.1096/fasebj.27.1_supplement.lb284.Peer-Reviewed Original ResearchIntestinal iron absorptionIron absorptionDietary proteinProtein dietMRNA expressionWhole-body iron homeostasisDuodenum of ratsDMT1 mRNA expressionBody iron homeostasisGenetic absenceExperimental animalsLevel of expressionResponsive elementBrush border membraneRatsIron-responsive elementRecent dataDietCritical regulatorIron homeostasisBorder membraneSignificant changesExpressionIron importerAnimalsThe effect of selective amino acid supplementation on calcium absorption during a low protein diet
Bihuniak J, Sullivan R, Simpson C, Caseria D, O'Brien K, Kerstetter J, Insogna K. The effect of selective amino acid supplementation on calcium absorption during a low protein diet. The FASEB Journal 2013, 27: lb283-lb283. DOI: 10.1096/fasebj.27.1_supplement.lb283.Peer-Reviewed Original ResearchLow-protein dietIntestinal Ca absorptionCa absorptionProtein dietProtein intakeDietary protein intakeHigh-protein dietAmino acid supplementationCalcium absorptionAcid supplementationDay 5Dibasic amino acidsAA supplementationYoung womenControl dietRandom orderPhysiologic rangeDietary proteinIntakeDietSupplementationAmino acidsAromatic amino acidsCa economyLys supplementation
2011
Dietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression
Cucchi C, Gaffney‐Stomberg E, Sun B, O'Brien K, Kerstetter J, Insogna K. Dietary protein upregulates fractional intestinal iron absorption and directly increases DMT1 transcript and protein expression. The FASEB Journal 2011, 25: lb193-lb193. DOI: 10.1096/fasebj.25.1_supplement.lb193.Peer-Reviewed Original ResearchProtein dietDietary proteinProtein expressionCaco-2 BBe cellsIntestinal Fe absorptionFe absorption studiesIntestinal iron absorptionTranscript expressionAmino acid-free mediumDMT1 protein levelsAmino acid treatmentDMT1 protein expressionProtein doseIron absorptionBBE cellsIron deficiencyAcid-free mediumRatsProtein levelsDietDMT1 transcriptsAmino acidsFe absorptionWestern analysisKey regulatorDietary protein increases intestinal calcium absorption in part by increasing tight junction expression of Claudin‐2
Gaffney‐Stomberg E, Cucchi C, Sun B, Bataille A, Renfro L, Kerstetter J, Insogna K. Dietary protein increases intestinal calcium absorption in part by increasing tight junction expression of Claudin‐2. The FASEB Journal 2011, 25: 983.30-983.30. DOI: 10.1096/fasebj.25.1_supplement.983.30.Peer-Reviewed Original ResearchHigh-protein dietCaco-2 BBe cellsClaudin-2 expressionCa absorptionCalcium absorptionProtein dietClaudin-2BBE cellsFemale Sprague-Dawley ratsIntestinal calcium absorptionDietary proteinSprague-Dawley ratsTight junction expressionCa transportTight junction proteinsTranscellular Ca transportDietary protein effectsCa-transporting proteinsDuodenal mucosaAcute effectsDay 7MRNA expressionJunction expressionJunction proteinsAmino acids
2010
Six Weeks of a Low Protein Diet Impairs Calcium Homeostasis
Sullivan R, Rosewater I, Caseria D, O'Brien K, Kerstetter J, Insogna K. Six Weeks of a Low Protein Diet Impairs Calcium Homeostasis. The FASEB Journal 2010, 24: lb356-lb356. DOI: 10.1096/fasebj.24.1_supplement.lb356.Peer-Reviewed Original ResearchLow-protein dietProtein dietCalcium absorptionDual-stable calcium isotopesImpairs calcium homeostasisIntestinal calcium absorptionNegative bone balanceWeek 6.5Diet intervention trialLong-term ingestionUrinary calciumMetabolic kitchenBone lossVitamin DIntervention trialsStable calcium isotopesCalcium metabolismBone resorptionBone balanceIntestinal absorptionWeek 1Skeletal turnoverCalcium homeostasisMineral homeostasisYoung womenA High Protein Diet Induces Intestinal Iron Transporter Expression and Improves Iron Absorption in Rats
Cucchi C, Gaffney‐Stomberg E, Sun B, Kerstetter J, Insogna K. A High Protein Diet Induces Intestinal Iron Transporter Expression and Improves Iron Absorption in Rats. The FASEB Journal 2010, 24: 229.1-229.1. DOI: 10.1096/fasebj.24.1_supplement.229.1.Peer-Reviewed Original ResearchDietary proteinIron absorptionTransporter expressionHigh casein dietExpression of DMT1Iron transporter expressionFe absorptionVs. 5Kruskal-Wallis testMicroarray screenCasein dietMineral homeostasisLevel of expressionProtein dietRatsLittle dataDMT1 expressionQ-PCRDietBalance studiesExpressionHomeostasisGroupDMT1CYBRD1The Effect of Dietary Protein on Intestinal Calcium Absorption in Rats
Gaffney-Stomberg E, Sun BH, Cucchi CE, Simpson CA, Gundberg C, Kerstetter JE, Insogna KL. The Effect of Dietary Protein on Intestinal Calcium Absorption in Rats. Endocrinology 2010, 151: 1071-1078. PMID: 20147526, PMCID: PMC2840679, DOI: 10.1210/en.2009-0744.Peer-Reviewed Original ResearchConceptsIntestinal Ca absorptionCa absorptionHigh-protein dietUrinary calciumBrush border membrane vesiclesProtein intakeFemale Sprague-Dawley ratsProtein dietProtein intake resultsIntestinal calcium absorptionDietary protein intakeHigh protein intakeSprague-Dawley ratsLow-protein groupHigh protein groupDietary protein effectsCalcium absorptionAcute increaseDawley ratsDuodenal mucosaIntake resultsRatsCa balanceTranscellular CaDietary protein
2009
A Rodent Model to Evaluate the Effect of Dietary Protein on Intestinal Calcium Absorption
Gaffney‐Stomberg E, Cucchi C, Sun B, Simpson C, Kerstetter J, Insogna K. A Rodent Model to Evaluate the Effect of Dietary Protein on Intestinal Calcium Absorption. The FASEB Journal 2009, 23: 726.1-726.1. DOI: 10.1096/fasebj.23.1_supplement.726.1.Peer-Reviewed Original ResearchHigh-protein dietLow-protein groupCa absorptionProtein dietBone turnoverBrush border membrane vesiclesIntestinal Ca absorptionIntestinal calcium absorptionDietary proteinVitamin D metabolitesLow protein intakeCa transportLow-protein dietSerum PTHSerum osteocalcinHr urineCalcium absorptionD metabolitesSerum CaDuodenal mucosaUrine CaRodent modelsAnimal modelsProtein intakeCa metabolism
2007
Dietary protein increases intestinal calcium absorption and improves bone balance: An hypothesis
Kerstetter J, Gaffney E, O'Brien K, Caseria D, Insogna K. Dietary protein increases intestinal calcium absorption and improves bone balance: An hypothesis. International Congress Series 2007, 1297: 204-216. DOI: 10.1016/j.ics.2006.08.027.Peer-Reviewed Original ResearchBone mineral densityIntestinal calcium absorptionHigh-protein dietUrinary calciumBone lossCalcium absorptionProtein dietAccelerated bone lossMost cross-sectional studiesDietary proteinCross-sectional studyLow-protein dietBone turnoverBone resorptionMineral densityBone balanceCalcium studiesCalcium retentionSalutary effectsBone originPrincipal outcomeDietSignificant reductionCalciumResorption
2004
High Protein Diets, Calcium Economy, and Bone Health
Kerstetter J, O'Brien K, Insogna K. High Protein Diets, Calcium Economy, and Bone Health. Topics In Clinical Nutrition 2004, 19: 57-70. DOI: 10.1097/00008486-200401000-00010.Peer-Reviewed Original ResearchHigh-protein dietProtein dietDecrease bone densityUrinary calcium excretionDietary proteinHigh dietary proteinCalcium excretionUrinary calciumBone healthSkeletal healthCalcium economyBone resorptionBone densityLong-term consequencesDietBoneSignificant scientific dataPhysiological mechanismsHealthHumansExcretionResorptionProtein
2003
Low Protein Intake: The Impact on Calcium and Bone Homeostasis in Humans
Kerstetter JE, O'Brien KO, Insogna KL. Low Protein Intake: The Impact on Calcium and Bone Homeostasis in Humans. Journal Of Nutrition 2003, 133: 855s-861s. PMID: 12612169, DOI: 10.1093/jn/133.3.855s.BooksConceptsIntestinal calcium absorptionLow-protein dietCalcium absorptionHigh-protein dietProtein dietSecondary hyperparathyroidismUrinary calciumBone resorptionDietary proteinAdditional urinary calciumDietary protein resultsSerum parathyroid hormoneNutrition intervention trialCalcium balance studiesRecent epidemiological studiesLow protein intakeNutrition intervention studiesParathyroid hormoneBone lossSkeletal healthIntervention trialsCalcium metabolismSkeletal fracturesBone densityLong-term consequences
1999
Changes in Bone Turnover in Young Women Consuming Different Levels of Dietary Protein1
Kerstetter J, Mitnick M, Gundberg C, Caseria D, Ellison A, Carpenter T, Insogna K. Changes in Bone Turnover in Young Women Consuming Different Levels of Dietary Protein1. The Journal Of Clinical Endocrinology & Metabolism 1999, 84: 1052-1055. PMID: 10084594, DOI: 10.1210/jcem.84.3.5552.Peer-Reviewed Original ResearchUrinary N-telopeptide excretionBone-specific alkaline phosphataseN-telopeptide excretionUrinary calcium excretionBone turnoverLow-protein dietCalcium excretionUrinary calciumProtein dietBone resorptionDietary proteinYoung womenHealthy young womenNegative calcium balanceLow protein intakeHigh-protein dietAlkaline phosphataseSecondary hyperparathyroidismSerum PTHSerum osteocalcinDihydroxyvitamin DCalcium balanceProtein intakeCompensatory increaseDay 4
1992
Cross-cultural association between dietary animal protein and hip fracture: A hypothesis
Abelow B, Holford T, Insogna K. Cross-cultural association between dietary animal protein and hip fracture: A hypothesis. Calcified Tissue International 1992, 50: 14-18. PMID: 1739864, DOI: 10.1007/bf00297291.Peer-Reviewed Original ResearchConceptsHip fracture incidenceFracture incidenceDietary animal proteinHigh animal protein dietTotal caloric intakeAnimal protein dietHip fractureDietary calciumFracture ratesCaloric intakeBone bufferingFracture associationMetabolic acid productionBone dissolutionProtein dietFurther studiesPositive associationProtein consumptionAnimal proteinIncidenceAssociationAnimal protein consumptionRecent studies