The Cooley Lab works to provide a strong and supportive environment for training at all levels.
- Dissertation Research: We welcome graduate students interested in doing a rotation in the lab.
- Postdoctoral Training: Postdocs interested in developing an independent research program using Drosophila oogenesis are welcome to apply. Must be willing to apply for fellowship support.
- Undergraduate Research: The lab hosts Yale College students for research during the academic year and summer months.
- Internships: Opportunities for summer research for high school students or undergraduates are available.
Here are a few ideas for rotation projects that have emerged from recent work in the lab:
·Live imaging of cytokinesis during Drosophila sperm development using fluorescent proteins and swept-field microscopy to study previously unknown intermediates of ring canal formation (see figure).
·Analyze Focused Ion Beam Scanning Electron Microscopy (FIB SEM) data of wild type and mutant testes to extract high-resolution 3-D information for the transformation to ring canals.
·Localized biotinylation to identify proteins at the RC/plasma membrane interface. Use APEX-tagged, membrane proximal proteins to biotinylate neighboring proteins.
·Generate and analyze dual fluorescent protein reporters to directly visualize tissue-specific stop codon readthrough in vivo.
·Determine the function of carboxy terminal extensions by analyzing CRISPR-mediated deletions of ORF2 in Drosophila genes.
·Investigate the mechanism of stop codon readthrough with a candidate gene shRNA screen.
Please contact Lynn Cooley for more information, or to make an appointment to talk about an ongoing or new project.