The lab is looking to hire a lab manager to help us with daily operations in order to keep things running smoothly. An excellent candidate should have both a love for science and the type of organized mind necessary for maintaining strains, lab supplies, and the happiness of colleagues. A detailed description of the position follows below. For additional questions about the position, please reach out via email to email@example.com
- Have excellent interpersonal and management skills with a professional and customer service oriented demeanor. Excellent verbal and written communication skills, which may include business and technical writing, editing and proofreading.
- Manage the ordering process and common lab purchases of the Colón-Ramos Lab. We define common lab purchases as those which are used by most/all members of the lab and are not specific to any particular project. Handle the purchase of needed common lab reagents in the medical stockroom and through Workday. Anticipate the lab use of reagents to ensure the consistent availability of common reagents; including but not limited to microcentrifuge tubes, pipette tips, serological pipettes, miniprep and gel cleanup kits, culture tubes, commonly used enzymes, etc. Order printer ink.
- Freeze bacterial and nematode strains generated by the members of the Colón-Ramos Lab. The process consists in obtaining the strain and associated information from the lab member that generated the strain(s), freezing each strain in aliquots for our -80C and liquid N2 stock boxes, adding the relevant information in the corresponding database and performing test thaws in the case of the nematode strains to confirm viability of the frozen stock. Develop standardized notation methods for both databases. Link plasmid database to plasmid maps. Maintain and curate the two databases. Sending lab strains to other labs. Experience working with laboratory data/information management systems or laboratory databases would be beneficial.
- Prepare common reagents used in the Colón-Ramos Lab for the smooth operation of the lab. This includes preparing solutions such as 50X and 1X TAE, M9 and S Buffer, 1M KPO4 pH6, 1M CaCl2, 1M MgSO4; prepare aliquots for antibiotics (Kanamycin, Ampicillin, Chloramphenicol), nematode anaesthetics (Levamisole & Muscimol) and commonly used enzymes (2X GoTaq, LR and BP Clonases [Gateway]). Make plates for bacterial selection (Kanamycin or Ampicillin). Autoclave and refill pipette tip boxes. Make unseeded plates.
- Keep the lab clean and the equipment in working condition, ensuring the lab runs smoothly. The person would be in charge of keeping the common areas clean and in working condition -- with special emphasis in the chemical reagents/gel room. Maintain the incubators and bench mite-free by coordinating periodical cleaning and baking days. Cleaning the -80C freezers. Take care of the compound, dissecting and injection scopes in the lab, and all the other equipment (centrifuges, PCR machines, etc) and contact the corresponding vendor for service when problems arise. Making sure all equipment is functional, including replacing microscope bulbs and parts when malfunction, cleaning objectives, and replacing biohazard containers once filled.
- Be the research liaison for the Colón-Ramos Lab. Interface with the sales reps. When other labs request strains or other reagents from our lab, prepare the transfer of said materials and ship them using eShipGlobal or similar structures Yale provides. Be the EHS contact person and our lab safety officer, which entails overseeing safety and compliance requirements for chemicals and hazardous materials use. Interface with contractors and facilities when needed.
- Train new lab members in basic nematode husbandry and molecular biology techniques. Basic nematode husbandry includes seeding plates with OP50, making your own pick, picking worms, distinguishing L4s, males and adults, learning how to cross, injecting and freezing. Basic molecular biology techniques can include PCR for genotyping worms, Gateway cloning, bacterial transformation, etc. Train new lab members in using the ordering system for their project-specific purchases. Be the knowledge source of know-how for the lab. Ideally, after a week of mentorship, the trainee should be able to perform most common techniques in the Colón-Ramos Lab.
- In coordination with the IT specialists from our departments, keep the lab computers updated and protected. Develop strategies to ensure robust data storage. Maintain lab website.
- Generate nematode strains for broad lab use. When a transgenic strain is identified to be broadly useful for the lab, carry out the integration of the source extrachromosomal array line and generate at least 3 independent integrated lines, mapping the chromosome in which it got integrated. If the broadly useful strain will be the product of a CRISPR edit, design the necessary reagents (crRNAs and repair template), generate and validate the lines.
- Provide assistance with specific lab projects at the level of experimental design or composing research articles, when determined by Daniel Colón-Ramos.