Total RNA for Gene Expression Profiling
- Total RNA of very high quality (28S:18S = 2:1, OD 260/280 > 1.8, OD 260/230 > 1.8) in DEPC-H2O is the best way to begin.
- Samples should be cleaned with Qiagen Rneasy columns, including Trizol-isolated RNA. Samples for micro RNA expression should be isolated and purified with Qiagen miRNeasy Mini kit (cat. no. 217004).
- Dnase-treatment is highly recommended as RNeasy columns alone may not completely remove genomic DNA.
- Total RNA isolated from whole blood needs to be globin-reduced. We recommend Ambion GLOBINclear kit (cat. no. AM1980, AM1981).
- Samples that need to be concentrated at our facility will be assessed an additional fee.