Mass Spectrometry
FT-ICR
Uses
- Exact/Accurate mass of small molecules, peptides, oligos (RNA/DNA), lipids, intact proteins, drugs, etc. for determining elemental composition
- Structural Elucidation of small molecule; Use of multi-fragmentation techniques of FT-ICR for small molecules to determine structural information.
- Protein Post Translational Modification
- Protein Identification & Peptide sequencing
- Comparative protein/peptide profiling
Important Links
Figure 1: FT-ICR Small Molecule Exact Mass
Advantages of FT-ICR
- Ultra High Resolution for separation of molecular masses less than 0.002 Da
- High Mass Accuracy (<3ppm with Ext. Calibration) for elemental assignment
- Multi-fragmentation capabilities for structural elucidation and protein PTM analysis
- Protein Identification & Peptide sequencing
- Comparative protein/peptide profiling.