Protein/Peptide Fractionation

Avidin Chromatography

Peptides that are biotinylated (e.g. ICAT tagged peptides) can be fractionated using Avidin Chromatography on the AB Vision work station or using a cartridge system. The labeled peptides are eluted in 0.4% TFA, 30% acetonitrile, which is dried prior to LC-MS/MS analysis. Typical sample amounts are 50 to 400μg of complex protein mixtures.