Template FAQ
How much plasmid DNA do you need for a standard premixed tube reaction?
- 1,000-1,500 ng ds plasmid DNA template
- 2 µl 4 µM primer
- x µl sterile water
- 18 µl total volume
How much plasmid DNA do you need for a high volume premixed 96-well reaction?
- 1,000-1,500 ng ds plasmid DNA template
- 2 µl 4 µM primer
- x µl sterile water
- 18 µl total volume
How much PCR product do you need for a standard premixed tube reaction?
- 100-300bp:40-50ng
- 300- 500bp:40-160ng
- 500bp- 1kb:500ng
- 2 µl 4 µM primer
- x µl sterile water
- 18 µl total volume
How much PCR product do you need for a high volume premixed 96-well reaction?
- 100-300bp:40-50ng
- 300- 500bp:40-160ng
- 500bp- 1kb:500ng
- 2 µl 4 µM primer
- x µl sterile water
- 18 µl total volume
Should I resuspend template in TE or H
2O?
How long is the average read?
Template quality will affect length of read. Average read length is 500bp, however, lengths of 650-750bp are routinely achieved. We often see lengths of 800bp-1100bp.