Correlation between the amount of protein analyzed and the accuracy of MW determination using DAWN® DSP and OPTILAB DSP detectors.

Look at the actual data in the "micro analyses" graph

Protein	Amount loaded [micrograms]	number of runs	Pred. MW [kDa] a	Average experimental MW [kDa]	Range of experimental accuracy [%]b	Standard deviation [kDa]
Ovalbumin	150	4	42.8	42.4	0.2 to 1.6	0.3
	100	7	42.8	42.3	0.2 to 2.4	0.8
	45-50	4	42.8	41.6	0.5 to 5.8	1
	6-10	5	42.8	42.9	1.4 to 4.5	1.5
Transferrin	100	3	75.2	76.5	0.7 to 3.2	1
	8	5	75.2	76.3	0.3 to 5.2	2

(column: TSK GEL G3000_SWXL [TosoHaas], buffer: 20 mM phosphate, 150 mM NaCl, pH=7.5).

^a predicated MW was calculated based on protein sequence as retrieved from the ExPASy molecular biology WWW server of the Swiss Institute of Bioinformatics (SIB). http://expasy.hcuge.ch/

^b Experimental accuracy (%) is calculated as an absolute value of {100*[(Average Experimental MW-predicted MW)/predicted MW]}