2023
Efficient reconstruction of cell lineage trees for cell ancestry and cancer
Jang Y, Fasching L, Bae T, Tomasini L, Schreiner J, Szekely A, Fernandez T, Leckman J, Vaccarino F, Abyzov A. Efficient reconstruction of cell lineage trees for cell ancestry and cancer. Nucleic Acids Research 2023, 51: e57-e57. PMID: 37026484, PMCID: PMC10250207, DOI: 10.1093/nar/gkad254.Peer-Reviewed Original ResearchConceptsLineage treesCell ancestryCell lineage treesFirst cell divisionStem cell linesPluripotent stem cell lineLineage reconstructionInduced pluripotent stem cell lineCell divisionCancer progressionLineage representationCell linesMosaic mutationsHuman skin fibroblastsTreesMutationsAncestrySkin fibroblastsMultiple cellsGenomeLineagesZygotesLinesFibroblastsCells
2017
One thousand somatic SNVs per skin fibroblast cell set baseline of mosaic mutational load with patterns that suggest proliferative origin
Abyzov A, Tomasini L, Zhou B, Vasmatzis N, Coppola G, Amenduni M, Pattni R, Wilson M, Gerstein M, Weissman S, Urban AE, Vaccarino FM. One thousand somatic SNVs per skin fibroblast cell set baseline of mosaic mutational load with patterns that suggest proliferative origin. Genome Research 2017, 27: 512-523. PMID: 28235832, PMCID: PMC5378170, DOI: 10.1101/gr.215517.116.Peer-Reviewed Original ResearchConceptsSomatic mosaicismFibroblast cellsSingle-cell whole-genome amplificationAllele frequenciesNumber of SNVsNormal cell proliferationCell proliferationWhole genome amplificationStem cell linesPluripotent stem cell lineHealthy human tissuesDe novo variantsCancer mutationsHigh-resolution analysisMutational loadPCR experimentsSkin fibroblast cellsMutational signaturesHiPSC linesDe novoGenomeNovo variantsFibroblast populationsCell linesSomatic SNVs
2000
The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina
Baas D, Bumsted KM, Martinez JA, Vaccarino FM, Wikler KC, Barnstable CJ. The subcellular localization of OTX2 is cell-type specific and developmentally regulated in the mouse retina. Brain Research 2000, 78: 26-37. PMID: 10891582, DOI: 10.1016/s0169-328x(00)00060-7.Peer-Reviewed Original ResearchMeSH Keywords3T3 CellsAnimalsAntibodiesBlotting, WesternCell NucleusCytoplasmGene Expression Regulation, DevelopmentalHomeodomain ProteinsHumansMiceMice, Inbred StrainsNerve Tissue ProteinsOtx Transcription FactorsPC12 CellsPigment Epithelium of EyeRabbitsRatsRetinal Ganglion CellsRetinal Rod Photoreceptor CellsTeratocarcinomaTrans-ActivatorsTransfectionTumor Cells, CulturedConceptsSubcellular localizationTranscription factorsHomeodomain-containing proteinCell fate determinationHomeodomain transcription factorCytoplasm of rodsFate determinationCell fateOtx2 proteinSubcellular distributionOtx2Retinal pigment epithelial cellsCell typesRod photoreceptorsPigment epithelial cellsRetinal developmentCytoplasmCell linesAdult eyesEpithelial cellsCentral nervous systemImmature rodsProteinCellsDifferential distribution
1985
gamma-Aminobutyric acid- and benzodiazepine-induced modulation of [35S]- t-butylbicyclophosphorothionate binding to cerebellar granule cells
Gallo V, Wise BC, Vaccarino F, Guidotti A. gamma-Aminobutyric acid- and benzodiazepine-induced modulation of [35S]- t-butylbicyclophosphorothionate binding to cerebellar granule cells. Journal Of Neuroscience 1985, 5: 2432-2438. PMID: 4032005, PMCID: PMC6565307, DOI: 10.1523/jneurosci.05-09-02432.1985.Peer-Reviewed Original ResearchConceptsGamma-aminobutyric acidIntact cerebellar granule cellsCerebellar granule cellsGranule cellsT-butylbicyclophosphorothionateEffects of muscimolDose-dependent fashionAbsence of diazepamNeuroblastoma cell linesBenzodiazepine receptor ligandsSpecific bindingMicroM diazepamConvulsant activityCerebellar astrocytesMuscimolDiazepamLocke's solutionTotal radioactivityPrimary culturesReceptor ligandsCell linesReceptor complexCarboxylic acid methylesterInhibitionCells