Daniela Tirziu

Bio Profile

Daniela Tirziu, PhD

Research Associate 2, HSS

Research Interests

Cell Communication; Cell Enlargement; Endothelium, Vascular; Endothelium-Dependent Relaxing Factors; Gene Regulatory Networks; Heart Diseases; Heart Failure; MicroRNAs

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Tirziu Lab

My laboratory is interested in bi-directional crosstalk between vascular endothelium and cardiomyocytes that regulates cardiac size and function. As an alternative to myocyte-driven hypertrophy in response to hemodynamic stress we recently reported a cross-talk loop induction of myocardial hypertrophy by expanding vascular endothelium in the absence of traditional hypertrophy stimuli. This reveals a new and unexplored role played by the endothelium in regulation of adult organ growth and size that would be of great interest in formulating new therapeutic angiogenic approaches to the treatment of heart disease.

Our hypothesis is that an increase in vascular endothelium in the adult heart results in increased nitric oxide (NO) production that in turn drives the growth of cardiomyocytes by sustained ubiquitination of the negative regulator of G protein signaling subtype 4 (RGS4) and derepression of the hypertrophic program via heterotrimeric G protein signaling. To investigate this crosstalk we use cell culture and conditional mouse models.


Pfau D, Thorn SL, Zhang J, Mikush N, Renaud JM, Klein R, deKemp RA, Wu X, Hu X, Sinusas AJ, Young LH, Tirziu D: Angiotensin Receptor Neprilysin Inhibitor Attenuates Myocardial Remodeling and Improves Infarct Perfusion in Experimental Heart Failure. Sci Rep. 2019 Apr 8; 2019 Apr 8. PMID: 30962467

Memi F, Tirziu D, Papangeli I: Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization. J Vis Exp. 2018 Sep 15; 2018 Sep 15. PMID: 30272664

Li N, Hwangbo C, Jaba IM, Zhang J, Papangeli I, Han J, Mikush N, Larrivée B, Eichmann A, Chun HJ, Young LH, Tirziu D: miR-182 Modulates Myocardial Hypertrophic Response Induced by Angiogenesis in Heart. Sci Rep. 2016 Feb 18; 2016 Feb 18. PMID: 26888314

Sakurai T, Lanahan A, Woolls MJ, Li N, Tirziu D, Murakami M: Live cell imaging of primary rat neonatal cardiomyocytes following adenoviral and lentiviral transduction using confocal spinning disk microscopy. J Vis Exp. 2014 Jun 24; 2014 Jun 24. PMID: 24998400

Stacy MR, Yu DY, Maxfield MW, Jaba IM, Jozwik BP, Zhuang ZW, Lin BA, Hawley CL, Caracciolo CM, Pal P, Tirziu D, Sampath S, Sinusas AJ: Multimodality imaging approach for serial assessment of regional changes in lower extremity arteriogenesis and tissue perfusion in a porcine model of peripheral arterial disease. Circ Cardiovasc Imaging. 2014 Jan; 2013 Oct 29. PMID: 24170237

Jaba IM, Zhuang ZW, Li N, Jiang Y, Martin KA, Sinusas AJ, Papademetris X, Simons M, Sessa WC, Young LH, Tirziu D: NO triggers RGS4 degradation to coordinate angiogenesis and cardiomyocyte growth. J Clin Invest. 2013 Apr. PMID: 23454748

Tirziu D, Jaba IM, Yu P, Larrivée B, Coon BG, Cristofaro B, Zhuang ZW, Lanahan AA, Schwartz MA, Eichmann A, Simons M: Endothelial nuclear factor-κB-dependent regulation of arteriogenesis and branching. Circulation. 2012 Nov 27; 2012 Oct 22. PMID: 23091063

Stan C, Tîrziu D, Lupaşcu S: [A new risk factor in glaucoma?]. Oftalmologia. 2011. PMID: 21774391

Tirziu D, Giordano FJ, Simons M: Cell communications in the heart. Circulation. 2010 Aug 31. PMID: 20805439

Tirziu D, Simons M: Endothelium as master regulator of organ development and growth. Vascul Pharmacol. 2009 Jan-Feb; 2008 Sep 5. PMID: 18804188

Anbanandam A, Albarado DC, Tirziu DC, Simons M, Veeraraghavan S: Molecular basis for proline- and arginine-rich peptide inhibition of proteasome. J Mol Biol. 2008 Dec 5; 2008 Sep 16. PMID: 18823992

Tirziu D, Simons M: Endothelium-driven myocardial growth or nitric oxide at the crossroads. Trends Cardiovasc Med. 2008 Nov. PMID: 19345317

Tirziu D, Chorianopoulos E, Moodie KL, Palac RT, Zhuang ZW, Tjwa M, Roncal C, Eriksson U, Fu Q, Elfenbein A, Hall AE, Carmeliet P, Moons L, Simons M: Myocardial hypertrophy in the absence of external stimuli is induced by angiogenesis in mice. J Clin Invest. 2007 Nov. PMID: 17975666

Muinck ED, Nagy N, Tirziu D, Murakami M, Gurusamy N, Goswami SK, Ghatpande S, Engelman RM, Simons M, Das DK: Protection against myocardial ischemia-reperfusion injury by the angiogenic Masterswitch protein PR 39 gene therapy: the roles of HIF1alpha stabilization and FGFR1 signaling. Antioxid Redox Signal. 2007 Apr. PMID: 17280485

Tkachenko E, Elfenbein A, Tirziu D, Simons M: Syndecan-4 clustering induces cell migration in a PDZ-dependent manner. Circ Res. 2006 Jun 9; 2006 May 4. PMID: 16675718

Post MJ, Sato K, Murakami M, Bao J, Tirziu D, Pearlman JD, Simons M: Adenoviral PR39 improves blood flow and myocardial function in a pig model of chronic myocardial ischemia by enhancing collateral formation. Am J Physiol Regul Integr Comp Physiol. 2006 Mar; 2005 Oct 27. PMID: 16254127

Tirziu D, Moodie KL, Zhuang ZW, Singer K, Helisch A, Dunn JF, Li W, Singh J, Simons M: Delayed arteriogenesis in hypercholesterolemic mice. Circulation. 2005 Oct 18. PMID: 16230502

Tirziu D, Simons M: Angiogenesis in the human heart: gene and cell therapy. Angiogenesis. 2005; 2005 Nov 25. PMID: 16308736

Tîrziu D, Dobrian A, Tasca C, Simionescu M, Simionescu N: Intimal thickenings of human aorta contain modified reassembled lipoproteins. Atherosclerosis. 1995 Jan 6. PMID: 7772061

Simionescu N, Sima A, Dobrian A, Tirziu D, Simionescu M: Pathobiochemical changes of the arterial wall at the inception of atherosclerosis. Curr Top Pathol. 1993. PMID: 8125021