Daniel Greif

Bio Profile

Daniel Greif, MD

Associate Professor Term

Research Interests

Aorta; Aortic Stenosis, Subvalvular; Atherosclerosis; Cardiology; Cerebral Hemorrhage; Developmental Biology; Hypertension, Pulmonary; Myofibroblasts; Pulmonary Fibrosis; Vascular Diseases

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Greif Lab

My laboratory investigates blood vessel morphogenesis, the maintenance of the adult blood vessel and diseases of the vasculature. To this end, we utilize fundamental biochemical, genetic and developmental biological approaches, including lineage and clonal analyses. We initially investigated the origins of the smooth muscle and adventitial cells of the pulmonary artery and have delineated with cellular resolution the patterns of cell differentiation, proliferation, recruitment and migration in the developing pulmonary arterial wall. Furthermore, we have initiated a genetic dissection of the molecular processes and signals that underlie blood vessel formation and have demonstrated a role of the platelet derived growth factor pathway in this process.


Chakraborty R, Saddouk FZ, Carrao AC, Krause DS, Greif DM, Martin KA: Promoters to Study Vascular Smooth Muscle. Arterioscler Thromb Vasc Biol. 2019 Apr. PMID: 30727757

Ntokou A, Greif DM: Vagal Nerve Stimulation for Pulmonary Hypertension: Some Promise, Some Skepticism. JACC Basic Transl Sci. 2018 Oct; 2018 Nov 12. PMID: 30456338

Dave JM, Mirabella T, Weatherbee SD, Greif DM: Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain. Dev Cell. 2018 Nov 5. PMID: 30399337

Dubrac A, Künzel SE, Künzel SH, Li J, Chandran RR, Martin K, Greif DM, Adams RH, Eichmann A: NCK-dependent pericyte migration promotes pathological neovascularization in ischemic retinopathy. Nat Commun. 2018 Aug 27; 2018 Aug 27. PMID: 30150707

Misra A, Feng Z, Chandran RR, Kabir I, Rotllan N, Aryal B, Sheikh AQ, Ding L, Qin L, Fernández-Hernando C, Tellides G, Greif DM: Integrin beta3 regulates clonality and fate of smooth muscle-derived atherosclerotic plaque cells. Nat Commun. 2018 May 25; 2018 May 25. PMID: 29802249

Sheikh AQ, Saddouk FZ, Ntokou A, Mazurek R, Greif DM: Cell Autonomous and Non-cell Autonomous Regulation of SMC Progenitors in Pulmonary Hypertension. Cell Rep. 2018 Apr 24. PMID: 29694892

Dave JM, Mirabella T, Weatherbee SD, Greif DM: Pericyte ALK5/TIMP3 Axis Contributes to Endothelial Morphogenesis in the Developing Brain. Dev Cell. 2018 Mar 26; 2018 Feb 15. PMID: 29456135

Misra A, Feng Z, Zhang J, Lou ZY, Greif DM: Using In Vivo and Tissue and Cell Explant Approaches to Study the Morphogenesis and Pathogenesis of the Embryonic and Perinatal Aorta. J Vis Exp. 2017 Sep 12; 2017 Sep 12. PMID: 28930997

Jiao Y, Li G, Li Q, Ali R, Qin L, Li W, Qyang Y, Greif DM, Geirsson A, Humphrey JD, Tellides G: mTOR (Mechanistic Target of Rapamycin) Inhibition Decreases Mechanosignaling, Collagen Accumulation, and Stiffening of the Thoracic Aorta in Elastin-Deficient Mice. Arterioscler Thromb Vasc Biol. 2017 Sep; 2017 Jul 27. PMID: 28751568

Greif DM, Eichmann A: Basic research: Where do new endothelial cells come from in the injured heart? Nat Rev Cardiol. 2017 Sep; 2017 Aug 3. PMID: 28770866

Mazurek R, Dave JM, Chandran RR, Misra A, Sheikh AQ, Greif DM: Vascular Cells in Blood Vessel Wall Development and Disease. Adv Pharmacol. 2017; 2016 Oct 14. PMID: 28212800

Misra A, Sheikh AQ, Kumar A, Luo J, Zhang J, Hinton RB, Smoot L, Kaplan P, Urban Z, Qyang Y, Tellides G, Greif DM: Integrin β3 inhibition is a therapeutic strategy for supravalvular aortic stenosis. J Exp Med. 2016 Mar 7; 2016 Feb 8. PMID: 26858344

Sheikh AQ, Misra A, Rosas IO, Adams RH, Greif DM: Smooth muscle cell progenitors are primed to muscularize in pulmonary hypertension. Sci Transl Med. 2015 Oct 7. PMID: 26446956

Seidelmann SB, Lighthouse JK, Greif DM: Development and pathologies of the arterial wall. Cell Mol Life Sci. 2014 Jun; 2013 Sep 27. PMID: 24071897

Greif DM, Eichmann A: Vascular biology: Brain vessels squeezed to death. Nature. 2014 Apr 3; 2014 Mar 26. PMID: 24670635

Sheikh AQ, Lighthouse JK, Greif DM: Recapitulation of developing artery muscularization in pulmonary hypertension. Cell Rep. 2014 Mar 13; 2014 Feb 27. PMID: 24582963

Kim J, Kang Y, Kojima Y, Lighthouse JK, Hu X, Aldred MA, McLean DL, Park H, Comhair SA, Greif DM, Erzurum SC, Chun HJ: An endothelial apelin-FGF link mediated by miR-424 and miR-503 is disrupted in pulmonary arterial hypertension. Nat Med. 2013 Jan; 2012 Dec 23. PMID: 23263626

Greif DM, Kumar M, Lighthouse JK, Hum J, An A, Ding L, Red-Horse K, Espinoza FH, Olson L, Offermanns S, Krasnow MA: Radial construction of an arterial wall. Dev Cell. 2012 Sep 11. PMID: 22975322

London NR, Zhu W, Bozza FA, Smith MC, Greif DM, Sorensen LK, Chen L, Kaminoh Y, Chan AC, Passi SF, Day CW, Barnard DL, Zimmerman GA, Krasnow MA, Li DY: Targeting Robo4-dependent Slit signaling to survive the cytokine storm in sepsis and influenza. Sci Transl Med. 2010 Mar 17. PMID: 20375003

SenBanerjee S, Lin Z, Atkins GB, Greif DM, Rao RM, Kumar A, Feinberg MW, Chen Z, Simon DI, Luscinskas FW, Michel TM, Gimbrone MA Jr, García-Cardeña G, Jain MK: KLF2 Is a novel transcriptional regulator of endothelial proinflammatory activation. J Exp Med. 2004 May 17; 2004 May 10. PMID: 15136591

Greif DM, Sacks DB, Michel T: Calmodulin phosphorylation and modulation of endothelial nitric oxide synthase catalysis. Proc Natl Acad Sci U S A. 2004 Feb 3; 2004 Jan 21. PMID: 14736917

Goetz DJ, Greif DM, Shen J, Luscinskas FW: Cell-cell adhesive interactions in an in vitro flow chamber. Methods Mol Biol. 1999. PMID: 10098131

Goetz DJ, Greif DM, Ding H, Camphausen RT, Howes S, Comess KM, Snapp KR, Kansas GS, Luscinskas FW: Isolated P-selectin glycoprotein ligand-1 dynamic adhesion to P- and E-selectin. J Cell Biol. 1997 Apr 21. PMID: 9128259