Daniel Greif, MD

Associate Professor Term

Research Interests

Aorta; Aortic Stenosis, Subvalvular; Cardiology; Cerebral Hemorrhage; Hypertension, Pulmonary; Pulmonary Fibrosis; Vascular Diseases; Developmental Biology; Atherosclerosis

Research Organizations

Internal Medicine: Cardiovascular Medicine

Animal Organogenesis

Faculty Research

Vascular Biology and Therapeutics Program

Office of Cooperative Research

Research Summary

Cardiovascular disease is the number one cause of death globally. My laboratory utilizes multi-disciplinary approaches to investigate how blood vessels initially form, are maintained and go awry in disease. Our research spans from cultured cells to mouse models to human samples. We aim to gain critical insights into the pathogenesis of diverse cardiovascular pathologies and leverage these insights into novel therapeutics for human disease.

Specialized Terms: Vascular biology; Vascular smooth muscle; Vessel wall; Developmental biology; Clonal analysis; Lineage analysis; Pulmonary artery hypertension; Aorta; Intracranial hemorrhage; Atherosclerosis.

Extensive Research Description

My laboratory investigates blood vessel morphogenesis, the maintenance of the adult blood vessel and diseases of the vasculature. To this end, we utilize fundamental biochemical, genetic and developmental biological approaches. We initially investigated the origins of the smooth muscle and adventitial cells of the pulmonary artery and have delineated with cellular resolution the patterns of cell differentiation, proliferation, recruitment and migration in the developing pulmonary arterial wall. Furthermore, we have initiated a genetic dissection of the molecular processes and signals that underlie blood vessel formation and have demonstrated a role of the platelet derived growth factor pathway in this process.

Our ongoing and planned studies of vessel development, maintenance and disease use similar fundamental approaches. For instance, we are studying the morphogenesis of the walls of other vessels, such as the aorta and cerebral vasculature, and comparing and contrasting their morphogenesis with that of the pulmonary artery. In addition, little is known about the maintenance of blood vessels, and we are embarking on a study to evaluate the patterns of cell turnover, proliferation and migration as well as the underlying mechanisms in the adult vessel wall. Moreover, diseases of the vasculature are thought to largely involve a recapitulation of developmental programs, and we are applying our approaches to study animal models of vascular diseases that involve ectopic and aberrant smooth muscle cells, such as atherosclerosis, supravalvular aortic stenosis, restenosis, intracranial hemorrhage and pulmonary hypertension. We have recently extended our studies to lung fibrosis which is an important cause of hypoxia and hence pulmonary hypertension. Finally, we are studying clinical samples obtained from patients with vascular and lung diseases and relating them to our findings in animal models and cultured cells.

Current Research Projects:

  • Excess smooth muscle in pulmonary hypertension: cell origins and recruitment.
  • Aortic wall development and disease (atherosclerosis, supravalvular aortic stenosis): progenitor cell specification, migration and differentiation.
  • Mural cell signaling and blood brain barrier formation: implications for intracerebral hemorrhage.
  • Alveolar myofibroblasts in development and disease

Selected Publications

Full List of PubMed Publications

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Contact Info

Daniel Greif, MD
Patient Care Locations
Yale CardiologyYale New Haven Shoreline Medical Center
111 Goose Lane, Ste Suite 2400

Guilford, CT 06437
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Yale CardiologyYale Physicians Building
800 Howard Avenue, Ste 4th Floor

New Haven, CT 06519
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Yale CardiologyLaboratory for Medicine and Pediatrics (LMP)
15 York Street

New Haven, CT 06510
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Yale Heart and Vascular Outpatient Services
84 North Main Street, Fl 2

Branford, CT 06405
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Lab Location
7th Floor300 George Street
New Haven, CT 06511
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Mailing Address
Cardiovascular MedicinePO Box 208017
New Haven, CT 06520-8017

Greif Lab