2024
Levels of Cytokines in Leptospirosis Patients with Different Serovars and rfb Locus
Senavirathna I, Jayasundara D, Warnasekara J, Kappagoda C, Agampodi S. Levels of Cytokines in Leptospirosis Patients with Different Serovars and rfb Locus. Journal Of Interferon & Cytokine Research 2024, 44: 80-93. PMID: 38377491, PMCID: PMC10880283, DOI: 10.1089/jir.2023.0091.Peer-Reviewed Original ResearchConceptsRfb locusGenome sequencePacBio RS II systemBacterial cell wallCulture-positive samplesLipopolysaccharide synthesisRapid AnnotationLeptospira borgpetersenii</i>,Genomic dataMicroscopic agglutination testSubsystem TechnologyLociCell wallSerovarsIsolated culturesSevere clinical manifestationsCytokine responsesLeptospira interrogans serovar autumnalisClinical manifestationsGenomeSamples of cultureSequenceRFBLevels of cytokinesSerum cytokine concentrations
2022
Demographic, exposure, clinical, biochemical and diagnostic data of febrile patients recruited for the largest field study on leptospirosis in Sri Lanka
Agampodi S, Warnasekara J, Siribaddana S, Kularatna S, Gamage C, Jayasundara D, Senevirathna I, Srimantha S, Kappagoda C, Weerawansa P, Pilapitiya S, Lokunarangoda N, Sarathchandra C, Senanayake H, Matthias M, Vinetz J. Demographic, exposure, clinical, biochemical and diagnostic data of febrile patients recruited for the largest field study on leptospirosis in Sri Lanka. Data In Brief 2022, 43: 108378. PMID: 35770027, PMCID: PMC9234077, DOI: 10.1016/j.dib.2022.108378.Peer-Reviewed Original ResearchDiagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting
Warnasekara J, Srimantha S, Kappagoda C, Jayasundara D, Senevirathna I, Matthias M, Agampodi S, Vinetz J. Diagnostic method-based underestimation of leptospirosis in clinical and research settings; an experience from a large prospective study in a high endemic setting. PLOS Neglected Tropical Diseases 2022, 16: e0010331. PMID: 35377883, PMCID: PMC9009773, DOI: 10.1371/journal.pntd.0010331.Peer-Reviewed Original ResearchConceptsMicroscopic agglutination testProspective hospital-based studyLeptospirosis casesQuantitative PCRHigh endemic settingHospital-based studyClinical case definitionLarge prospective studiesDisease burden estimatesPublic health burdenStandard diagnostic testMonths of observationUndifferentiated feverAcute leptospirosisProspective studyCase definitionEndemic settingsHealth burdenTimely diagnosisBurden estimatesDiagnostic valueSignificant human mortalityAgglutination testStudy settingDiagnostic tests
2021
Improving the leptospirosis disease burden assessment by including ambulatory patients from outpatient departments: a cross-sectional study
Warnasekara J, Aberathna P, Nanayakkara G, Vinetz J, Agampodi S. Improving the leptospirosis disease burden assessment by including ambulatory patients from outpatient departments: a cross-sectional study. F1000Research 2021, 9: 1129. PMID: 34123371, PMCID: PMC8145224, DOI: 10.12688/f1000research.26202.2.Peer-Reviewed Original ResearchConceptsOutpatient departmentOPD patientsAmbulatory patientsDisease burdenPrimary care centersProvincial General HospitalCases of leptospirosisCross-sectional studyDisease burden assessmentMicroscopic agglutination testPublic health implicationsQuantitative polymerase chain reactionOPD settingRegional Public HospitalOPD visitsLeptospirosis patientsFever patientsPatient numbersHospital inpatientsPolymerase chain reactionCare centerGeneral HospitalBurden assessmentPatientsSame period
2020
Improving the leptospirosis disease burden assessment by including ambulatory patients from outpatient departments: a cross-sectional study
Warnasekara J, Aberathna P, Nanayakkara G, Vinetz J, Agampodi S. Improving the leptospirosis disease burden assessment by including ambulatory patients from outpatient departments: a cross-sectional study. F1000Research 2020, 9: 1129. DOI: 10.12688/f1000research.26202.1.Peer-Reviewed Original ResearchOutpatient departmentDisease burden assessmentOPD patientsAmbulatory patientsBurden assessmentPrimary care centersProvincial General HospitalCases of leptospirosisCross-sectional studyPrevalence of leptospirosisMicroscopic agglutination testPublic health implicationsDisease burden estimationQuantitative polymerase chain reactionOPD settingRegional Public HospitalOPD visitsTreatable diagnosisLeptospirosis patientsFever patientsPatient numbersHospital inpatientsDisease burdenPolymerase chain reactionCare centerCytokine response in human leptospirosis with different clinical outcomes: a systematic review
Senavirathna I, Rathish D, Agampodi S. Cytokine response in human leptospirosis with different clinical outcomes: a systematic review. BMC Infectious Diseases 2020, 20: 268. PMID: 32264832, PMCID: PMC7137275, DOI: 10.1186/s12879-020-04986-9.Peer-Reviewed Original ResearchConceptsDifferent clinical outcomesCross-sectional studyCytokine responsesIL-10Clinical outcomesSystematic reviewDisease severityHealth Quality Assessment ToolComparative cross-sectional studyTNF-α levelsMethodsA systematic reviewMeasurement of cytokinesEnzyme-linked immunosorbent assayMicroscopic agglutination testPublic health professionalsQuality assessment toolMild leptospirosisCytokine stormElevated cytokinesObservational cohortLeptospirosis patientsProspective studyIL-1βIL-6IL-8
2019
Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study
Agampodi S, Warnasekara J, Jayasundara D, Senawirathna I, Gamage C, Kularatne S, Siribaddana S, Maththias M, Vinetz J. Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study. BMJ Open 2019, 9: e027850. PMID: 31511281, PMCID: PMC6738675, DOI: 10.1136/bmjopen-2018-027850.Peer-Reviewed Original ResearchConceptsEthics Review CommitteeMultilocus sequence typingOutpatient departmentClinico-epidemiological studyMulticentre prospective studyDay of admissionMicroscopic agglutination testPresence of diseaseHospital stayFebrile patientsMedical wardsProspective studySri Lankan isolatesRisk factorsVenous bloodDisease progressionEthical clearanceHigh incidenceAetiological aspectsPatientsAgglutination testMolecular epidemiologyPatient contextBedside inoculationLeptospirosis
2016
Redefining Gold Standard Testing for Diagnosing Leptospirosis: Further Evidence from a Well-Characterized, Flood-Related Outbreak in Sri Lanka.
Agampodi SB, Dahanayaka NJ, Nöckler K, Mayer-Scholl A, Vinetz JM. Redefining Gold Standard Testing for Diagnosing Leptospirosis: Further Evidence from a Well-Characterized, Flood-Related Outbreak in Sri Lanka. American Journal Of Tropical Medicine And Hygiene 2016, 95: 531-536. PMID: 27402521, PMCID: PMC5014254, DOI: 10.4269/ajtmh.16-0033.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayMicroscopic agglutination testIgG enzyme-linked immunosorbent assayQuantitative polymerase chain reactionImmunoglobulin G (IgG) ELISAHouse enzyme-linked immunosorbent assayDays of illnessAcute-phase seraCases of leptospirosisAvailable enzyme-linked immunosorbent assayGold standard testingMAT positive samplesDiagnostic test resultsAcute specimensAcute phasePatient populationPhase seraPolymerase chain reactionBlood samplesSerological sensitivityAgglutination testImmunosorbent assayLeptospirosisLeptospira kirschneriDominant serovars
2012
Predictors of the development of myocarditis or acute renal failure in patients with leptospirosis: An observational study
Dassanayake D, Wimalaratna H, Nandadewa D, Nugaliyadda A, Ratnatunga CN, Agampodi SB. Predictors of the development of myocarditis or acute renal failure in patients with leptospirosis: An observational study. BMC Infectious Diseases 2012, 12: 4. PMID: 22243770, PMCID: PMC3292442, DOI: 10.1186/1471-2334-12-4.Peer-Reviewed Original ResearchConceptsAcute renal failureRenal failureClinical featuresMicroscopic agglutination testOdds ratioTeaching hospitalExact testComplication of leptospirosisDevelopment of myocarditisLaboratory measuresCommon clinical featuresVaried clinical presentationsProspective descriptive studyPredictors of severityFisher's exact testMann-Whitney U testLogistic regression modelsPain 6ResultsSixty-twoLaboratory parametersClinical presentationLaboratory variablesLaboratory predictorsLeptospirosis patientsCase definition
2009
Evaluation of surveillance case definition in the diagnosis of leptospirosis, using the Microscopic Agglutination Test: a validation study
Dassanayake D, Wimalaratna H, Agampodi SB, Liyanapathirana VC, Piyarathna T, Goonapienuwala BL. Evaluation of surveillance case definition in the diagnosis of leptospirosis, using the Microscopic Agglutination Test: a validation study. BMC Infectious Diseases 2009, 9: 48. PMID: 19386085, PMCID: PMC2679753, DOI: 10.1186/1471-2334-9-48.Peer-Reviewed Original ResearchConceptsSurveillance case definitionMicroscopic agglutination testCase definitionNegative predictive valuePredictive valueClinical featuresMAT titresAgglutination testDays of feverAcute febrile illnessMethodsThe study populationTeaching Hospital KandyPositive predictive valueDiagnosis of leptospirosisPositive MAT resultsFebrile illnessMedical wardsResultsA totalHospital KandyStudy populationBlood samplesConclusionThis studyPatientsDiagnostic accuracyLeptospirosis