2024
A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes
Zhang L, Wang D, Shi P, Li J, Niu J, Chen J, Wang G, Wu L, Chen L, Yang Z, Li S, Meng J, Ruan F, He Y, Zhao H, Ren Z, Wang Y, Liu Y, Shi X, Wang Y, Liu Q, Li J, Wang P, Wang J, Zhu Y, Cheng G. A naturally isolated symbiotic bacterium suppresses flavivirus transmission by Aedes mosquitoes. Science 2024, 384: eadn9524. PMID: 38669573, DOI: 10.1126/science.adn9524.Peer-Reviewed Original ResearchConceptsColony forming unitsFlavivirus envelope proteinCommensal microbiotaIrreversible conformational changesGlucose dehydrogenaseMosquito gutConformational changesGut lumenPrevent viral infectionGutBacteriumFlavivirus transmissionEnvelope proteinsVector competencePrevent viral entryViral entryRefractory to infectionDengue virusForming unitsField mosquitoesTransmission of dengue virusFlavivirusesMosquitoesSemifield conditionsAdult mosquitoesA mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission
Wang Z, Nie K, Liang Y, Niu J, Yu X, Zhang O, Liu L, Shi X, Wang Y, Feng X, Zhu Y, Wang P, Cheng G. A mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission. The EMBO Journal 2024, 43: 1690-1721. PMID: 38378891, PMCID: PMC11066113, DOI: 10.1038/s44318-024-00056-x.Peer-Reviewed Original ResearchMyeloid cellsExpression of neutrophil chemoattractantsViral transmissionInflux of myeloid cellsMosquito bite siteSalivary componentsInflux of neutrophilsSkin-resident macrophagesPotential prophylactic targetsFlavivirus transmissionNF-kB signalingMechanism of actionProphylactic targetsSystemic disseminationRecruits proteinsSalivary factorsLocal infectionNeutrophil chemoattractantMammalian hostsEfficient viral transmissionPhytochemical resveratrolDengue virusBite siteTransmission of ZikaProtein
2022
Neighboring mutation‐mediated enhancement of dengue virus infectivity and spread
Chen L, Zhang X, Guo X, Peng W, Zhu Y, Wang Z, Yu X, Shi H, Li Y, Zhang L, Wang L, Wang P, Cheng G. Neighboring mutation‐mediated enhancement of dengue virus infectivity and spread. EMBO Reports 2022, 23: e55671. PMID: 36197120, PMCID: PMC9638853, DOI: 10.15252/embr.202255671.Peer-Reviewed Original ResearchLipases secreted by a gut bacterium inhibit arbovirus transmission in mosquitoes
Yu X, Tong L, Zhang L, Yang Y, Xiao X, Zhu Y, Wang P, Cheng G. Lipases secreted by a gut bacterium inhibit arbovirus transmission in mosquitoes. PLOS Pathogens 2022, 18: e1010552. PMID: 35679229, PMCID: PMC9182268, DOI: 10.1371/journal.ppat.1010552.Peer-Reviewed Original ResearchConceptsJapanese encephalitis virusYellow fever virusZika virusDengue virusSindbis virusGlobal public healthBroad-spectrum virucidal activityEffective prophylacticLipase activityViral envelope structureViral infectionSevere human diseasesEncephalitis virusAedes aegypti midgutVirucidal activityEtiological agentTremendous burdenFever virusVirusMost arbovirusesPublic healthVector-borne diseasesAegypti midgutDiseaseVirucidal ability
2019
Host serum iron modulates dengue virus acquisition by mosquitoes
Zhu Y, Tong L, Nie K, Wiwatanaratanabutr I, Sun P, Li Q, Yu X, Wu P, Wu T, Yu C, Liu Q, Bian Z, Wang P, Cheng G. Host serum iron modulates dengue virus acquisition by mosquitoes. Nature Microbiology 2019, 4: 2405-2415. PMID: 31527795, DOI: 10.1038/s41564-019-0555-x.Peer-Reviewed Original ResearchConceptsSerum ironDengue virusVirus acquisitionDengue virus infectionIron-deficient miceDengue virus prevalenceIron metabolism pathwaysViral loadIron supplementationA. aegypti mosquitoesHigh prevalenceVirus infectionArbovirus infectionHuman donorsIron deficiencyInfectionBlood componentsGut epitheliumReactive oxygen speciesSusceptibility of mosquitoesVirus prevalenceAegypti mosquitoesBlood mealHuman bloodVirus
2016
Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes
Liu J, Liu Y, Nie K, Du S, Qiu J, Pang X, Wang P, Cheng G. Flavivirus NS1 protein in infected host sera enhances viral acquisition by mosquitoes. Nature Microbiology 2016, 1: 16087. PMID: 27562253, PMCID: PMC5003325, DOI: 10.1038/nmicrobiol.2016.87.Peer-Reviewed Original ResearchConceptsNonstructural protein 1Japanese encephalitis virus nonstructural protein 1Dengue virusDENV nonstructural protein 1Flavivirus nonstructural protein 1Lethal DENV challengeNS1 proteinReceptor-deficient miceVirus nonstructural protein 1Flavivirus NS1 proteinAcquisition of virusDENV challengeActive immunizationFlaviviral diseasesImmune barrierHost serumViral transferInfected mammalian hostsViral acquisitionMosquito midgutMiceProtein 1Virus acquisitionImmunizationMosquito vectorsGlycosphingolipid GM3 is Indispensable for Dengue Virus Genome Replication
Wang K, Wang J, Sun T, Bian G, Pan W, Feng T, Wang P, Li Y, Dai J. Glycosphingolipid GM3 is Indispensable for Dengue Virus Genome Replication. International Journal Of Biological Sciences 2016, 12: 872-883. PMID: 27313500, PMCID: PMC4910605, DOI: 10.7150/ijbs.15641.Peer-Reviewed Original ResearchConceptsDENV infectionDengue virusB16 cellsDENV attachmentSynthetase inhibitorGM95 cellsPrevalent arthropodVirus infectionMouse melanoma B16 cellsMortality rateViral-host interactionsMelanoma B16 cellsMouse brainViral replicationInfectionVirus genome replicationViral diseasesGenome replicationViral genome replicationEndoplasmic reticulumGM3CellsViral replication complexInhibitorsGlycosphingolipids
2015
A Neuron-Specific Antiviral Mechanism Prevents Lethal Flaviviral Infection of Mosquitoes
Xiao X, Zhang R, Pang X, Liang G, Wang P, Cheng G. A Neuron-Specific Antiviral Mechanism Prevents Lethal Flaviviral Infection of Mosquitoes. PLOS Pathogens 2015, 11: e1004848. PMID: 25915054, PMCID: PMC4411065, DOI: 10.1371/journal.ppat.1004848.Peer-Reviewed Original ResearchMeSH KeywordsAedesAnimalsAntiviral AgentsApoptosisBrainCell LineCell MembraneDengue VirusDrosophila melanogasterDrosophila ProteinsEncephalitis Virus, JapaneseFemaleHost-Pathogen InteractionsHumansInsect ProteinsNerve Tissue ProteinsNeuronsPhylogenyProtein Interaction Domains and MotifsRecombinant ProteinsViral Envelope ProteinsVirus InternalizationConceptsMosquito nervous systemCentral nervous systemJapanese encephalitis virusNervous systemFlaviviral infectionsDengue virusSindbis virusAntiviral mechanismMembrane targetingDramatic pathologyPlasma membraneAnti-flavivirus activityMosquito cellsSurface envelope proteinMosquito lifespanNatural vectorCulex pipiensHuman viral diseasesNeural cellsFlavivirus infectionEnvelope proteinFunctional blockadeNeural apoptosisMosquito behaviorViral infection
2013
Mosquito Saliva Serine Protease Enhances Dissemination of Dengue Virus into the Mammalian Host
Conway MJ, Watson AM, Colpitts TM, Dragovic SM, Li Z, Wang P, Feitosa F, Shepherd DT, Ryman KD, Klimstra WB, Anderson JF, Fikrig E. Mosquito Saliva Serine Protease Enhances Dissemination of Dengue Virus into the Mammalian Host. Journal Of Virology 2013, 88: 164-175. PMID: 24131723, PMCID: PMC3911723, DOI: 10.1128/jvi.02235-13.Peer-Reviewed Original ResearchConceptsDengue virusDENV infectivityAedes aegypti salivaMurine lymph nodesSalivary gland extractsAegypti salivaPrevention of diseaseLymph nodesMosquito salivaDENV loadVivo modelSerine protease activityNovel targetViral attachmentViral infectivityVirus infectivitySerine protease inhibitorHeparan sulfate proteoglycanExtracellular matrix proteinsRNA knockdownProtease inhibitorsInfectivitySulfate proteoglycanCell migrationMammalian hosts
2011
Dengue Virus Capsid Protein Binds Core Histones and Inhibits Nucleosome Formation in Human Liver Cells
Colpitts TM, Barthel S, Wang P, Fikrig E. Dengue Virus Capsid Protein Binds Core Histones and Inhibits Nucleosome Formation in Human Liver Cells. PLOS ONE 2011, 6: e24365. PMID: 21909430, PMCID: PMC3164731, DOI: 10.1371/journal.pone.0024365.Peer-Reviewed Original ResearchConceptsDENV infectionDENV CHuman liver cellsDengue virusLiver cellsDengue virus infectionTime-dependent mannerSpecific antiviralsVirus infectionInfectionAlters levelsSerious human diseasesInteresting new roleViral RNANuclear presenceMature virus particlesVirus particlesHuman diseasesFlaviviral replicationCellsCellular responsesCapsid proteinStructural proteinsVaccine