2016
Calcium-Sensing Receptor in Breast Physiology and Cancer
Kim W, Wysolmerski JJ. Calcium-Sensing Receptor in Breast Physiology and Cancer. Frontiers In Physiology 2016, 7: 440. PMID: 27746743, PMCID: PMC5043011, DOI: 10.3389/fphys.2016.00440.BooksBreast cancer cellsPTHrP productionCancer cellsInhibits cell deathMammary epithelial cellsEpithelial cellsG-protein usageBreast cancer cell proliferationElevated extracellular calcium levelsOsteolytic bone metastasesNormal breast epithelial cellsNuclear pathwaysCalcium sensing receptorCalcium-sensing receptorBreast epithelial cellsBreast cancer progressionCancer cell proliferationExtracellular calcium levelsNormal breast cellsProtein secretionCell deathCancer progressionBone metastasesPTHrP secretionCellular proliferationCalcium-Sensing Receptor Promotes Breast Cancer by Stimulating Intracrine Actions of Parathyroid Hormone–Related Protein
Kim W, Takyar FM, Swan K, Jeong J, VanHouten J, Sullivan C, Dann P, Yu H, Fiaschi-Taesch N, Chang W, Wysolmerski J. Calcium-Sensing Receptor Promotes Breast Cancer by Stimulating Intracrine Actions of Parathyroid Hormone–Related Protein. Cancer Research 2016, 76: 5348-5360. PMID: 27450451, PMCID: PMC5026591, DOI: 10.1158/0008-5472.can-15-2614.Peer-Reviewed Original ResearchConceptsMMTV-PyMT miceBreast cancer cellsCaSR activationBone metastasesBreast cancerInhibited tumor cell proliferationOsteolytic bone metastasesCancer cellsHuman breast cancer cell linesCalcium-sensing receptorHuman breast cancer cellsHormone-related proteinTransgenic mouse modelBreast cancer cell linesMMTV-PyMT transgenic mouse modelBreast cancer progressionTumor cell proliferationTumor cell growthCancer cell linesPTHrP levelsTissue-specific disruptionHigh extracellular concentrationsPTHrP productionCASR genePTHrP expression
2013
Mammary-Specific Ablation of the Calcium-Sensing Receptor During Lactation Alters Maternal Calcium Metabolism, Milk Calcium Transport, and Neonatal Calcium Accrual
Mamillapalli R, VanHouten J, Dann P, Bikle D, Chang W, Brown E, Wysolmerski J. Mammary-Specific Ablation of the Calcium-Sensing Receptor During Lactation Alters Maternal Calcium Metabolism, Milk Calcium Transport, and Neonatal Calcium Accrual. Endocrinology 2013, 154: 3031-3042. PMID: 23782944, PMCID: PMC3749485, DOI: 10.1210/en.2012-2195.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAnimals, NewbornBiological TransportBone DevelopmentBone ResorptionCalciumCrosses, GeneticFemaleGene Expression RegulationLactationLactoglobulinsMammary Glands, AnimalMiceMice, KnockoutMice, TransgenicMilkParathyroid HormoneParathyroid Hormone-Related ProteinReceptors, Calcium-SensingRNA, MessengerConceptsRenal calcium excretionCalcium excretionCalcium metabolismBone resorptionCalcium transportMilk calciumMammary glandAccelerated bone resorptionMammary-specific ablationMaternal calcium metabolismDietary calcium intakeCalcium sensing receptorCalcium-sensing receptorSecretion of PTHrPSkeletal calcium storesMilk PTHrPCalcium intakePTH secretionPTHrP secretionMaternal boneBone turnoverBone metabolismOnset of lactationMaternal circulationSystemic calciumThe calcium-sensing receptor in the breast
VanHouten JN, Wysolmerski JJ. The calcium-sensing receptor in the breast. Best Practice & Research Clinical Endocrinology & Metabolism 2013, 27: 403-414. PMID: 23856268, PMCID: PMC3713417, DOI: 10.1016/j.beem.2013.02.011.BooksConceptsG-protein usageBreast cancer cellsCalcium-sensing receptorCalcium metabolismCancer cellsParathyroid hormone-related protein levelsBreast cellsMaternal calcium metabolismOsteolytic skeletal metastasesSystemic calcium metabolismEpithelial cellsNormal breast epithelial cellsFeed-forward cycleMalignant breast cellsNormal breast cellsBreast epithelial cellsSkeletal metastasesPTHrP productionPTHrP expressionMammary epithelial cellsCaSRCalcium transportProtein levelsTransport of calciumSupply of calcium
2009
The calcium-sensing receptor couples to Gαs and regulates PTHrP and ACTH secretion in pituitary cells
Mamillapalli R, Wysolmerski J. The calcium-sensing receptor couples to Gαs and regulates PTHrP and ACTH secretion in pituitary cells. Journal Of Endocrinology 2009, 204: 287-297. PMID: 20032198, PMCID: PMC3777408, DOI: 10.1677/joe-09-0183.Peer-Reviewed Original ResearchConceptsG protein-coupled receptorsACTH secretionCAMP productionProtein-coupled receptorsDifferent G proteinsCaR couplesAtT-20 cellsHormone secretionExtracellular calciumSystemic calcium homeostasisBiological functionsG proteinsBreast cancer cellsCell typesCalcium-sensing receptorCell growthReceptors coupleMalignant breast cellsCancer cellsKidney cellsBreast cellsCAR activationPituitary corticotrophsCalcium homeostasisAnterior pituitary
2008
Switching of G-protein Usage by the Calcium-sensing Receptor Reverses Its Effect on Parathyroid Hormone-related Protein Secretion in Normal Versus Malignant Breast Cells*
Mamillapalli R, VanHouten J, Zawalich W, Wysolmerski J. Switching of G-protein Usage by the Calcium-sensing Receptor Reverses Its Effect on Parathyroid Hormone-related Protein Secretion in Normal Versus Malignant Breast Cells*. Journal Of Biological Chemistry 2008, 283: 24435-24447. PMID: 18621740, PMCID: PMC2528989, DOI: 10.1074/jbc.m801738200.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBone NeoplasmsBreast NeoplasmsCell Line, TumorCyclic AMPCyclic AMP-Dependent Protein KinasesFemaleGTP-Binding Protein alpha SubunitsHumansHypercalcemiaLactationMammary Glands, AnimalMammary Neoplasms, AnimalMAP Kinase Signaling SystemMiceNeoplasm MetastasisNeoplasm ProteinsParathyroid Hormone-Related ProteinPregnancyReceptors, Calcium-SensingType C PhospholipasesConceptsG-protein usagePTHrP productionMammary epithelial cellsBreast cancer cellsBreast cellsMCF-7 cellsParathyroid hormone-related protein secretionParathyroid hormone-related proteinParathyroid hormone secretionCancer cellsMCF-7 human breast cancer cellsCalcium-sensing receptorHuman breast cancer cellsHormone-related proteinNormal mammary epithelial cellsRegulation of calciumMalignant breast cellsNormal breast cellsG protein-coupled receptorsG protein couplingBone metastasesPTHrP secretionHormone secretionBone metabolismBreast cancer
2007
The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk
VanHouten JN, Neville MC, Wysolmerski JJ. The Calcium-Sensing Receptor Regulates Plasma Membrane Calcium Adenosine Triphosphatase Isoform 2 Activity in Mammary Epithelial Cells: A Mechanism for Calcium-Regulated Calcium Transport into Milk. Endocrinology 2007, 148: 5943-5954. PMID: 17823248, PMCID: PMC7108505, DOI: 10.1210/en.2007-0850.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBiological TransportBlotting, WesternCalciumCells, CulturedEpithelial CellsFemaleFluorescent Antibody TechniqueGadoliniumIsoenzymesLactationMammary Glands, AnimalMiceMice, Inbred BALB CMicroscopy, ImmunoelectronMilkMilk ProteinsMutationOligonucleotide Array Sequence AnalysisPlasma Membrane Calcium-Transporting ATPasesReceptors, Calcium-SensingReverse Transcriptase Polymerase Chain ReactionConceptsPlasma membrane calcium ATPase isoform 2Mammary epithelial cellsMouse mammary epithelial cellsEpH4 mouse mammary epithelial cellsEpithelial cellsGenome-wide screening strategyApical plasma membraneATPase activityPotential downstream targetsATPase isoform 2Mouse mammary glandPlasma membrane preparationsDeafwaddler miceTransepithelial calcium transportPlasma membraneDownstream targetsGene knockdownPMCA2 activityCalcium transportIsoform 2Calcium-dependent ATPase activityCell membraneCultured primaryCalcium-sensing receptorPMCA2 expressionConversations between breast and bone: Physiological bone loss during lactation as evolutionary template for osteolysis in breast cancer and pathological bone loss after menopause
Wysolmerski J. Conversations between breast and bone: Physiological bone loss during lactation as evolutionary template for osteolysis in breast cancer and pathological bone loss after menopause. BoneKEy Reports 2007, 4: 209-225. DOI: 10.1138/20070268.Peer-Reviewed Original ResearchBone lossBone resorptionBreast cancerParathyroid hormone-related proteinLow estrogen levelsPathological bone resorptionPost-menopausal womenPathological bone lossCalcium-sensing receptorPhysiological bone lossHormone-related proteinSkeletal calcium storesHypothalamic hypogonadismPTHrP levelsEstrogen levelsPTHrP productionSkeletal demineralizationMineral metabolismSkeletal responseBone diseaseNew therapiesCalcium storesBone recoveryBreastCalcium transport
2005
The calcium-sensing receptor regulates PTHrP production and calcium transport in the lactating mammary gland
Ardeshirpour L, Dann P, Pollak M, Wysolmerski J, VanHouten J. The calcium-sensing receptor regulates PTHrP production and calcium transport in the lactating mammary gland. Bone 2005, 38: 787-793. PMID: 16377269, DOI: 10.1016/j.bone.2005.11.009.Peer-Reviewed Original ResearchConceptsCalcium-sensing receptorPTHrP productionMammary epithelial cellsMammary glandCalcium transportEpithelial cellsCalcium homeostasisParathyroid hormone-related proteinParathyroid hormone secretionProduction of PTHrPHormone-related proteinSystemic calcium homeostasisExtracellular calcium ionsTransepithelial calcium transportG protein-coupled receptorsHormone secretionParathyroid glandsBone massCalcium handlingRenal tubulesTransient lossGlandGenetic confirmationReceptorsSecretion
2004
The calcium-sensing receptor regulates mammary gland parathyroid hormone–related protein production and calcium transport
VanHouten J, Dann P, McGeoch G, Brown EM, Krapcho K, Neville M, Wysolmerski JJ. The calcium-sensing receptor regulates mammary gland parathyroid hormone–related protein production and calcium transport. Journal Of Clinical Investigation 2004, 113: 598-608. PMID: 14966569, PMCID: PMC338258, DOI: 10.1172/jci18776.Peer-Reviewed Original ResearchConceptsParathyroid hormone-related protein productionCalcium-sensing receptorMammary epithelial cellsEpithelial cellsCalcium transportMilk calcium contentExtracellular calcium concentrationSecretion of calciumTranscellular calcium transportPTHrP secretionPTHrP productionExtracellular calciumTransfer of calciumCalcium contentMammary glandCalcium concentrationProtein concentrationAvailability of calciumHomeostatic systemConcentration of calciumMilk osmolalitySecretionCalciumReceptorsThree-dimensional culture system
2003
A Syndrome of Hypocalciuric Hypercalcemia Caused by Autoantibodies Directed at the Calcium-Sensing Receptor
Kifor O, Moore FD, Delaney M, Garber J, Hendy GN, Butters R, Gao P, Cantor TL, Kifor I, Brown EM, Wysolmerski J. A Syndrome of Hypocalciuric Hypercalcemia Caused by Autoantibodies Directed at the Calcium-Sensing Receptor. The Journal Of Clinical Endocrinology & Metabolism 2003, 88: 60-72. PMID: 12519831, DOI: 10.1210/jc.2002-020249.Peer-Reviewed Original ResearchMeSH KeywordsAdultAutoantibodiesBlood Physiological PhenomenaBlotting, WesternCalciumCell LineEnzyme ActivationExtracellular SpaceFemaleHumansHypercalcemiaImmunoglobulinsInositol PhosphatesMiddle AgedMitogen-Activated Protein KinasesParathyroid GlandsParathyroid HormonePedigreePeptide FragmentsReceptors, Calcium-SensingReceptors, Cell SurfaceSyndromeConceptsPTH-dependent hypercalcemiaFamilial hypocalciuric hypercalcemiaHeterozygous inactivating mutationsHypocalciuric hypercalcemiaPTH releaseParathyroid cellsCaR's extracellular domainPatient seraExtracellular amino terminusAnti-CaR antiserumNormocalcemic control subjectsHuman parathyroid cellsBovine parathyroid cellsCalcium-sensing receptorAnti-CAR antibodyCaR-transfected HEK293 cellsInactivating mutationEffect of serumExtracellular domainAntithyroid antibodiesAutoimmune manifestationsEndocrine dysfunctionControl subjectsBlood calciumCaR gene