2005
Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites
Kocinsky HS, Girardi AC, Biemesderfer D, Nguyen T, Mentone S, Orlowski J, Aronson PS. Use of phospho-specific antibodies to determine the phosphorylation of endogenous Na+/H+ exchanger NHE3 at PKA consensus sites. American Journal Of Physiology. Renal Physiology 2005, 289: f249-f258. PMID: 15687252, DOI: 10.1152/ajprenal.00082.2004.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAntibodiesAntibodies, MonoclonalAntibody SpecificityCell LineChlorocebus aethiopsCOS CellsCyclic AMP-Dependent Protein KinasesDopamineElectrophoresis, Polyacrylamide GelImmunohistochemistryKidneyMaleMiceMice, Inbred BALB CMicroscopy, FluorescenceMicrovilliOpossumsPhosphorylationRatsRats, Sprague-DawleySodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersSubcellular FractionsTransfectionConceptsSerine 552Total NHE3Brush border membranePKA consensus sitesEffects of dopamineProximal tubule cellsWestern blot assaysOpossum kidney cellsBaseline phosphorylationPeptide antibodiesInhibition of NHE3Tubule cellsBlot assaysAntibodiesPhosphorylation of NHE3Phosphospecific antibodiesExchanger NHE3NHE3DopamineEndogenous NHE3Kidney cellsVivoPhospho-specific antibodiesTransfection studiesVitro
2004
Immunolocalization of NHE8 in rat kidney
Goyal S, Mentone S, Aronson PS. Immunolocalization of NHE8 in rat kidney. American Journal Of Physiology. Renal Physiology 2004, 288: f530-f538. PMID: 15522984, DOI: 10.1152/ajprenal.00229.2004.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsAntibodies, MonoclonalAntibody SpecificityBiotinChlorocebus aethiopsCOS CellsElectrophoresis, Polyacrylamide GelFluorescent Antibody Technique, DirectHybridomasImmunohistochemistryKidneyKidney Tubules, ProximalMembranesMiceMice, KnockoutMicrovilliMolecular Sequence DataRatsRats, Sprague-DawleySodium-Hydrogen Exchangers
2001
Association of Na+-H+ Exchanger Isoform NHE3 and Dipeptidyl Peptidase IV in the Renal Proximal Tubule*
Girardi A, Degray B, Nagy T, Biemesderfer D, Aronson P. Association of Na+-H+ Exchanger Isoform NHE3 and Dipeptidyl Peptidase IV in the Renal Proximal Tubule*. Journal Of Biological Chemistry 2001, 276: 46671-46677. PMID: 11590171, DOI: 10.1074/jbc.m106897200.Peer-Reviewed Original ResearchConceptsApical membraneExchanger Isoform NHE3Dipeptidyl peptidase IVN-terminal sequencingProtein complexesDPPIV enzyme activityRenal microvillus membrane vesiclesMicrovillar fractionMembrane populationsMembrane vesiclesPeptidase IVWestern blot analysisMicrovillus membrane vesiclesNHE3 surface expressionBrush borderImmunoelectron microscopyRabbit renal brush borderBlot analysisProteinEnzyme activitySurface expressionMicrovillus membraneNHE3Different poolsImmunoaffinity chromatography
1999
Specific Association of Megalin and the Na+/H+ Exchanger Isoform NHE3 in the Proximal Tubule*
Biemesderfer D, Nagy T, DeGray B, Aronson P. Specific Association of Megalin and the Na+/H+ Exchanger Isoform NHE3 in the Proximal Tubule*. Journal Of Biological Chemistry 1999, 274: 17518-17524. PMID: 10364184, DOI: 10.1074/jbc.274.25.17518.Peer-Reviewed Original Research
1998
Immunochemical characterization of Na+/H+exchanger isoform NHE4
Pizzonia J, Biemesderfer D, Abu-Alfa A, Wu M, Exner M, Isenring P, Igarashi P, Aronson P. Immunochemical characterization of Na+/H+exchanger isoform NHE4. American Journal Of Physiology 1998, 275: f510-f517. PMID: 9755122, DOI: 10.1152/ajprenal.1998.275.4.f510.Peer-Reviewed Original ResearchConceptsSubcellular localizationFusion proteinWestern blot analysisBlot analysisBroad tissue distributionBasolateral membraneTransport proteinsMembrane fractionProteinEpithelial cellsSkeletal muscleGradient centrifugationTissue distributionPercoll gradient centrifugationCellsGastric glandsNHE4MembraneImmunochemical characterizationMammalianLocalizationRat tissuesImmunochemical techniquesMonoclonal antibodiesPolypeptideMembrane Topology of NHE3 EPITOPES WITHIN THE CARBOXYL-TERMINAL HYDROPHILIC DOMAIN ARE EXOPLASMIC*
Biemesderfer D, DeGray B, Aronson P. Membrane Topology of NHE3 EPITOPES WITHIN THE CARBOXYL-TERMINAL HYDROPHILIC DOMAIN ARE EXOPLASMIC*. Journal Of Biological Chemistry 1998, 273: 12391-12396. PMID: 9575193, DOI: 10.1074/jbc.273.20.12391.Peer-Reviewed Original ResearchConceptsExchanger Isoform NHE3Membrane vesiclesAbsence of detergentPlasma membraneBorder membrane vesiclesCarboxyl-terminal hydrophilic domainBrush border membrane vesiclesCarboxyl-terminal domainMembrane topologyCytoskeletal effectorsCarboxyl terminusHigh-resolution immunocytochemistryExoplasmic surfaceCytoplasmic surfaceImmunoprecipitation studiesTransporter activityAmino acidsUltrathin cryosectionsImmunoblot analysisMembrane orientationHydrophilic domainsVesiclesMonoclonal antibodiesIntact microvilliGold technique
1997
Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney
Biemesderfer D, Rutherford PA, Nagy T, Pizzonia JH, Abu-Alfa AK, Aronson PS. Monoclonal antibodies for high-resolution localization of NHE3 in adult and neonatal rat kidney. American Journal Of Physiology 1997, 273: f289-f299. PMID: 9277590, DOI: 10.1152/ajprenal.1997.273.2.f289.Peer-Reviewed Original ResearchConceptsProximal tubulesRat kidneyMonoclonal antibodiesLoop of HenleHigh-resolution immunocytochemical studiesProximal tubule cellsPrevious immunochemical studiesIsoform-specific monoclonal antibodiesNeonatal rat kidneyTubule cellsImmunocytochemical studyThick limbImmunocytochemical techniquesKidneyLAP cellsRenal epitheliumNHE3Immunochemical studiesHigh-resolution immunocytochemical techniqueAdultsLater stagesNephron developmentExpression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat.
Rutherford PA, Pizzonia JH, Biemesderfer D, Abu-Alfa A, Reilly R, Aronson PS. Expression of Na(+)-H+ exchanger isoforms NHE1 and NHE3 in kidney and blood cells of rabbit and rat. Nephron 1997, 5: 490-7. PMID: 9438178.Peer-Reviewed Original ResearchConceptsNa-H exchangeNa-H exchangerBlood cellsRat kidneyNa-Li countertransportNHE1 protein expressionExpression of NHE1Diabetic nephropathyCountertransport activityIsoform-specific antibodiesMonoclonal antibodiesExchanger isoforms NHE1NHE3 expressionRenal brush border membraneProtein expressionBrush border membraneKidneyNHE isoformsRabbitsNHE3Blood cell membranesBrush border membrane fractionRatsIsoforms NHE1Antibodies
1992
Assembly of distinctive coated pit and microvillar microdomains in the renal brush border
Biemesderfer D, Dekan G, Aronson PS, Farquhar MG. Assembly of distinctive coated pit and microvillar microdomains in the renal brush border. American Journal Of Physiology 1992, 262: f55-f67. PMID: 1733297, DOI: 10.1152/ajprenal.1992.262.1.f55.Peer-Reviewed Original Research