2024
Endothelial-adipocyte Cx43 mediated gap junctions can regulate adiposity
Luse M, Dunaway L, Nyshadham S, Carvalho A, Sedovy M, Ruddiman C, Tessema R, Hirschi K, Johnstone S, Isakson B. Endothelial-adipocyte Cx43 mediated gap junctions can regulate adiposity. Function 2024, zqae029. PMID: 38984993, DOI: 10.1093/function/zqae029.Peer-Reviewed Original ResearchHigh-fat dietHigh-fat diet miceGap junctionsIncreased risk of cardiovascular diseaseUpregulation of fatty acid-binding protein 4Co-culture transwell systemPhosphorylation of Cx43Adipose endothelial cellsRisk of cardiovascular diseaseImproved lipid profileFatty acid binding protein 4Endothelial Cx43Adipocytes in vitroAdipose tissue functionBinding protein 4Post-translational modificationsEpididymal fat padsHeterocellular contactsSingle-cell RNA sequencingEndothelial dysfunctionSerine 368Cx43Heterocellular communicationLipid profileGenetic deletion
2023
Obesogenic diet disrupts tissue-specific mitochondrial gene signatures in the artery and capillary endothelium
Dunaway L, Luse M, Nyshadham S, Bulut G, Alencar G, Chavkin N, Cortese-Krott M, Hirschi K, Isakson B. Obesogenic diet disrupts tissue-specific mitochondrial gene signatures in the artery and capillary endothelium. Physiological Genomics 2023, 56: 113-127. PMID: 37982169, PMCID: PMC11281809, DOI: 10.1152/physiolgenomics.00109.2023.Peer-Reviewed Original ResearchConceptsSingle-cell RNA sequencingMitochondrial gene expressionGene expressionRNA sequencingOxidative phosphorylation gene expressionHigh-fat dietTranscription factor analysisSingle-nucleus RNAEndothelial cellsCapillary endothelial cellsAdipose endothelial cellsMitochondrial genesPhenotypic convergenceMesenteric endothelial cellsTranscriptional phenotypeBulk RNALipid homeostasisPeroxisome proliferator-activated receptorDistinct rolesPhenotypic shiftNovel mechanismMouse AdiposeProliferator-activated receptorResident tissuesDownregulation of PPAR