TAC Aria-B(S633)
Room: TAC S633
Contact:
Zhao.Zhao@yale.edu (203) 737-4715, (203) 785-7958 (office)
Technical Specifications
- High speed cell sorter
- Multilaser multiparameter analysis cytometer
- Three lasers, 10 colors
- Digital data collection using FACS DIVA software
- Automatic compensation (compensation controls are still needed)
- Capable of sorting human samples (equipped with Aerosol Evacuation System)
- Single cell sorting (all sizes of plates or microscope slides)
- Sterile sorting
- 4-way sorting
Violet Laser - 407nm
|
PMT |
Bandpass |
Long Pass |
Fluorochrome |
|
A |
530/30 |
502LP |
Alexa Fluor 430, CFP |
|
B |
450/40 |
Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP, Marina Blue |
Blue Laser - 488nm
|
PMT |
Bandpass |
Long Pass |
Fluorochrome |
|
A |
780/60 |
735LP |
PE-Cy7, PE-Alexa Fluor 750 |
|
B |
695/40 |
655LP |
PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA |
|
C |
610/20 |
595LP |
PE-Texas Red, PI (with PE and PE-Cy5.5) |
|
D |
576/26 |
556LP |
PE, PI (with PerCP) |
|
E |
530/30 |
502LP |
FITC, GFP, CFSE, YFP, Alexa Fluor 488, SYBR-14 |
|
F |
488/10 |
SSC |
Red Laser - 633nm
|
PMT |
Bandpass |
Long Pass |
Fluorochrome |
|
A |
780/60 |
735LP |
APC-Cy7, APC-Alexa 750 |
|
B |
710/25 |
685LP |
Alexa Fluor 680, Alexa Fluor 700 |
|
C |
660/20 |
APC, Alexa Fluor 647 |
*Please consult FACS Facility Staff or http://www.bdbiosciences.com/spectra/ to determine which fluorochromes cannot be used together due to high spectral overlaps.