LSR II TAC 5-UV ( TAC S533 )
Contact: diane.trotta@yale.edu or call 203-785-7958
- 355 nm (UV): BUV737, BUV395, DAPI
- 405 nm (Violet): CFP, Alexa Fluor 430, Pacific Blue, Marina Blue, Alexa Fluor 405
- 488 nm (Blue): FITC, PE, PerCP, Pe-Cy5 (aka CyChrome), PI, PerCp-Cy5.5, PE-Cy7, GFP, PE-Texas Red
- 633 nm (Red): APC, APC-Cy7, Alexa Fluor 647, Alexa 680
- Availability of UV gives a capability to perform Ca 2+ flux experiments.
UV Laser - 355nm 20 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 740/35 | 690LP | BUV737 |
| B
|
440/40 | 410LP | DAPI, Alexa Fluor 350
|
| C | 379/28 | BUV395 |
**DAPI and BUV395 Should not be used together.
Additional filters are necessary for Calcium Flux and Side Population detection, please see the staff about filters swaps.
Violet Laser - 405nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 710/50 | 690LP | BV711 |
| B | 610/20 | 595LP | BV605, Alexa Fluor 605, QDot605 |
| C | 525/50 | 505LP | Alexa Fluor 430, CFP, Am Cyan |
| D | 440/40 | Cascade Blue, Pacific Blue, DAPI, Hoechst, Alexa Fluor 405, BFP,
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Marina Blue, BV421
|
Blue Laser - 488nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 735LP | PE-Cy7, Alexa 750 PE |
| B | 670/14 | 635LP | PI (with PE), PerCP, PerCP-Cy5.5, PE-Cy5, EMA |
| C | 610/20 | 600LP | PE-Texas Red, PI (with PE and PE-Cy5.5) |
| D | 576/26 | 550LP | PE, PI (with PerCP), Pyronin |
| E | 530/30 | 505LP | FITC, GFP, CFSE, YFP, Alexa Fluor 488 |
| F | 488/10 | SSC |
Red Laser - 640nm 40 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 735LP | APC-Cy7, APC-Alexa Fluor 750 |
| B | 710/25 | 685LP | Alexa Fluor 680, Alexa Fluor 700 |
| C | 660/20 | APC, Alexa Fluor 647 |
*Please consult FACS Facility Staff or https://www.bdbiosciences.com/en-us/applications/research-applications/multicolor-flow-cytometry to determine which fluorochromes cannot be used together due to high spectral overlaps.