High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation
Li Y, Tingey M, Ruba A, Yang W. High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation. Nature Protocols 2020, 16: 532-560. PMID: 33318694, PMCID: PMC9382854, DOI: 10.1038/s41596-020-00440-x.Peer-Reviewed Original ResearchConceptsSpeed microscopySuper-resolution imaging techniquesDiffraction-limited resolutionComplex optical componentsSuper-resolution imagingSuper-resolution informationOptical componentsSuper-resolution microscopyBiological channelsSpeed imagingRotational symmetryFluorescent moleculesHigh spatiotemporal resolutionMicroscopy approachSymmetric structureImaging techniquesMicroscopySpatiotemporal resolutionEpifluorescence microscopeLive cellsResolutionSubcellular structures