2016
Precise Genome Modification Using Triplex Forming Oligonucleotides and Peptide Nucleic Acids
Bahal R, Gupta A, Glazer P. Precise Genome Modification Using Triplex Forming Oligonucleotides and Peptide Nucleic Acids. Advances In Experimental Medicine And Biology 2016, 93-110. DOI: 10.1007/978-1-4939-3509-3_6.Peer-Reviewed Original ResearchHomologous recombinationSite-specific genome editingPrecise genome modificationGenomic DNA sequencesSpecific genome editingTriplex technologySingle base pair mutationTriplex forming oligonucleotideBase pair mutationGenetic disordersGenome modificationNucleic acidsDefective protein synthesisGenome editingDNA sequencesDifferent genesPair mutationsProtein synthesisMutation sitesGenesSpecific sitesGene replacement therapyExpressionOligonucleotideMutagenesis
2006
Peptide Nucleic Acids as Agents to Modify Target Gene Expression and Function
Wang G, Glazer P. Peptide Nucleic Acids as Agents to Modify Target Gene Expression and Function. Medical Intelligence Unit 2006, 223-235. DOI: 10.1007/0-387-32956-0_14.Peer-Reviewed Original ResearchTarget gene expressionGene expressionMolecular mechanismsHuman diseasesGene expression regulationExpression regulationTranscription initiationDNA repairImportant genesMolecular basisTranscriptionGenesNucleic acidsExpressionAbility of PNAMutagenesisAttractive strategyPowerful methodBetter understandingRegulationMechanismPeptide nucleic acidBroad range
1999
Chromosomal mutations induced by triplex-forming oligonucleotides in mammalian cells
Vasquez K, Wang G, Havre P, Glazer P. Chromosomal mutations induced by triplex-forming oligonucleotides in mammalian cells. Nucleic Acids Research 1999, 27: 1176-1181. PMID: 9927753, PMCID: PMC148300, DOI: 10.1093/nar/27.4.1176.Peer-Reviewed Original ResearchConceptsTriplex-forming oligonucleotidesMutation reporter geneMultiple chromosomal copiesMutation frequencyMammalian chromosomesTriplex binding siteMammalian cellsChromosomal copyFibroblast cell lineChromosomal lociGenetic manipulationMouse fibroblast cell lineSequencing dataChromosomal mutationsDuplex DNAUntreated control cellsBinding sitesCell linesControl cellsSpecific recognitionMutagenesisMutationsT transversionSpecific sitesCellsGenome Modification by Triplex-Forming Oligonucleotides
Vasquez K, Glazer P. Genome Modification by Triplex-Forming Oligonucleotides. Perspectives In Antisense Science 1999, 2: 167-179. DOI: 10.1007/978-1-4615-5177-5_13.Peer-Reviewed Original ResearchSite-specific genome modificationGenome modificationSpecific DNA sequencesExpression of genesLevel of DNAMammalian cellsHomologous recombinationDNA sequencesGene replacementGene expressionLiving cellsTriplex technologyTriplex formingSpecific sitesDNARecombinationExpressionInitial stepCellsMutagenesisGenesMutationsModificationSitesSequence
1997
Elevated levels of mutation in multiple tissues of mice deficient in the DNA mismatch repair gene Pms2
Narayanan L, Fritzell J, Baker S, Liskay R, Glazer P. Elevated levels of mutation in multiple tissues of mice deficient in the DNA mismatch repair gene Pms2. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 3122-3127. PMID: 9096356, PMCID: PMC20332, DOI: 10.1073/pnas.94.7.3122.Peer-Reviewed Original ResearchConceptsDNA mismatch repair gene PMS2Multiple tissuesMutation reporter geneMismatch repair gene PMS2Role of mutagenesisMammalian homologGenomic integrityReporter geneRepeat sequencesPMS2 locusMononucleotide repeat sequencesGenetic instabilityLimited tissue distributionDNA mismatch repair genesRepair genesHereditary colon cancerNormal developmentSlippage errorsGenesMutagenic treatmentEssential roleMismatch repair genesMutagenesisMutation frequencyHybrid transgenic mice
1996
Mutagenesis in Mammalian Cells Induced by Triple Helix Formation and Transcription-Coupled Repair
Wang G, Seidman M, Glazer P. Mutagenesis in Mammalian Cells Induced by Triple Helix Formation and Transcription-Coupled Repair. Science 1996, 271: 802-805. PMID: 8628995, DOI: 10.1126/science.271.5250.802.Peer-Reviewed Original ResearchConceptsMammalian cellsSufficient binding affinitiesTranscription-coupled repairHuman cell extractsInhibition of transcriptionSimian virus 40 vectorXeroderma pigmentosum groupExcision repairGenetic instabilityTriple helix formationCell extractsTriplex-forming oligonucleotidesGroup B cellsDNA repair synthesisTranscriptionMutagenesisRepair synthesisTriplex DNAHelix formationTriplex formationTriple helixCellsBinding affinitiesTherapeutic applicationsB cellsTriplex‐Mediated, in vitro Targeting of Psoralen Photoadducts within the Genome of a Transgenic Mouse
Gunther E, Havre P, Gasparro F, Glazer P. Triplex‐Mediated, in vitro Targeting of Psoralen Photoadducts within the Genome of a Transgenic Mouse. Photochemistry And Photobiology 1996, 63: 207-212. PMID: 8657733, DOI: 10.1111/j.1751-1097.1996.tb03015.x.Peer-Reviewed Original ResearchConceptsPsoralen modificationMouse DNAGenomic mouse DNAPsoralen photoadductsSequence-specific bindingSequence-specific modificationNucleic acid secondary structureTarget site modificationMammalian genomesAcid secondary structureChromatin structureTriplex binding siteDNA repairTransgenic miceGenomeSequence specificitySecondary structureViral genomeSupF geneDNABinding sitesMutagenesisSite modificationSpecific sitesTriple helix
1995
Mutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links.
Gunther E, Yeasky T, Gasparro F, Glazer P. Mutagenesis by 8-methoxypsoralen and 5-methylangelicin photoadducts in mouse fibroblasts: mutations at cross-linkable sites induced by offoadducts as well as cross-links. Cancer Research 1995, 55: 1283-8. PMID: 7882323.Peer-Reviewed Original ResearchConceptsLambda phage shuttle vectorMutation reporter geneMammalian cellsCross-linkable sitesFibroblast cell lineMouse fibroblast cell lineReporter geneMolecular eventsShuttle vectorSpectrum of mutationsMouse fibroblastsPremutagenic lesionsSupF geneCross-link formationMutationsC transversionCell linesMutagenesisGenesApt sitePsoralen treatmentCellsSitesDNA
1993
Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide.
Havre P, Glazer P. Targeted mutagenesis of simian virus 40 DNA mediated by a triple helix-forming oligonucleotide. Journal Of Virology 1993, 67: 7324-31. PMID: 8230456, PMCID: PMC238196, DOI: 10.1128/jvi.67.12.7324-7331.1993.Peer-Reviewed Original ResearchConceptsTriple helix-forming oligonucleotidesTarget genesTranscription factor accessViral genomeTransient expression assaysSimian virus 40 DNASequence-specific mannerDNA sequence analysisGene functionFactor accessMammalian cellsGenetic manipulationHomopurine/homopyrimidineTargeted base pairsPromoter sitesTriple-helical DNAExpression assaysGenetic engineeringGene expressionTargeted mutationsSequence analysisMutagenesisHeritable effectsSV40 DNAGenome
1989
Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice
Summers W, Glazer P, Malkevich D. Lambda phage shuttle vectors for analysis of mutations in mammalian cells in culture and in transgenic mice. Mutation Research/Fundamental And Molecular Mechanisms Of Mutagenesis 1989, 220: 263-268. PMID: 2522589, DOI: 10.1016/0165-1110(89)90030-4.Peer-Reviewed Original ResearchConceptsMammalian DNALambda phage shuttle vectorForeign DNA sequencesLambda packaging extractsAnalysis of mutationsInfectious phage particlesMammalian cellsBacteriophage genomesGene sequencesDNA sequencesChromosomal sequencesBacterial systemsMammalian mutagenesisShuttle vectorLambda phageMolecular levelPhage particlesE. coliDNATransgenic miceSequenceMutationsGenomeMutagenesisPhages
1986
Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector.
Glazer P, Sarkar S, Summers W. Detection and analysis of UV-induced mutations in mammalian cell DNA using a lambda phage shuttle vector. Proceedings Of The National Academy Of Sciences Of The United States Of America 1986, 83: 1041-1044. PMID: 2937054, PMCID: PMC323006, DOI: 10.1073/pnas.83.4.1041.Peer-Reviewed Original ResearchConceptsMammalian cellsStable mouse L cell lineLambda phage shuttle vectorMouse cell DNACell DNAMammalian cell DNASupF geneMouse L cell lineDNA sequence analysisLambda phage vectorL cell lineMutant phageMouse cellsSequence analysisShuttle vectorUV-induced mutationsMultiple copiesUV mutagenesisPhage vectorEscherichia coliMutagenesisViable phagePhagesGenesT transition
1985
Direct and inducible mutagenesis in mammalian cells.
Summers W, Sarkar S, Glazer P. Direct and inducible mutagenesis in mammalian cells. Cancer Surveys 1985, 4: 517-28. PMID: 3916654.Peer-Reviewed Original ResearchConceptsMammalian cellsAnimal cellsAnimal virusesSimple eukaryotesInducible mutagenesisMutant geneSequence analysisShuttle vectorMutagenic pathwayMutagenesisDNA damageViral genomeWeigle mutagenesisMutagenic mechanismsDirect sequence analysisProkaryotesCellsBacteriaEukaryotesGenomeSimilar sitesRecent dataGenesExtrapolation of resultsPhages